Abstract
Objective: To investigate the expression difference of protein kinase B/Akt (Akt-1) between hepatocellular carcinoma (HCC) and adjacent normal liver tissues through the use of semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Northern blot. Methods: RT-PCR of 24 pairs of specimens and Northern blot of 4 pairs of specimens were performed to investigate the expression of Akt-1. Results: Akt-1 gene was overexpressed in 15 of 24 HCC (63.3%) by RT-PCR and in all HCC (4 paired tissues) by Northern blot. Conclusion: Akt-1 activation may play a role in the pathogenesis and progression of HCC. Akt-1 gene is reported to have changed in HCC for the first time. The precise relationship between Akt-1 and HCC is a matter of further investigation.
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Foundation item: This work was support by the National “863”High-Tech Program of China (No. Z19-01-01-02).
Biography: LIU Lian-xin (1970–), male, doctor of medicine, associate professor of surgery, First Clinical College, Harbin Medical University, majors in surgical and molecular oncology.
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Liu, Lx., Liu, Zh., Jiang, Hc. et al. Overexpression of Akt-1 gene in human hepatocellular carcinoma. Chin. J. Cancer Res. 14, 161–164 (2002). https://doi.org/10.1007/s11670-002-0036-1
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DOI: https://doi.org/10.1007/s11670-002-0036-1
Key words
- Hepatocellular carcinoma (HCC)
- Protein kinase B/Akt (Akt-1)
- Reverse transcription polymerase chain reaction (RT-PCR)
- Northern blot