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Energy metabolism disorder and myocardial injury in chronic myocardial ischemia with Qi deficiency and blood stasis syndrome based on 2-DE proteomics

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Abstract

Objective

To inquire the characteristic proteins in chronic myocardial ischemia by testing twodimensional electrophoresis (2-DE) map to explore the possible inherent pathological mechanism and the therapeutic intervention of qi deficiency and blood stasis syndrome.

Methods

Ameroid constrictor ring was placed on the first interval of left anterior descending coronary artery to prepare chronic myocardial ischemia model on Chinese miniature swine. Animals were randomly divided into sham group and model group with 10 animals in each group, respectively. The dynamic symptoms observation of the four diagnostic information was collected from 0 to 12 weeks. Echocardiography was employed to evaluate cardiac function and the degree of myocardial ischemia, 2-DE and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) were used to carry out proteomics research on animals. Enzyme-linked immunosorbent assay was applied to identify the relevant differential proteins on chronic myocardial ischemia with qi deficiency and blood stasis syndrome.

Results

The preliminary study found that at the 12th week, chronic myocardial ischemia with qi deficiency and blood stasis syndrome model was established stably. Compared with the sham group, there were 8 different proteins down-regulated, 22 proteins up-regulated significantly. After validated by MALDITOF-MS/MS, 11 protein spots were identified. Distinct proteins were mainly associated with energy metabolism and myocardial structural injury, including isocitrate dehydrogenase 3 (NAD+) alpha, NADH dehydrogenase (NAD) Fe-S protein 1, chain A (crystal structure of aldose reductase by binding domain reveals a new Nadph), heat shock protein 27 (HSP27), oxidoreductase (NAD-binding protein), antioxidant protein isoform, cardiac troponin T (cTnT), myosin (myosin light polypeptide), cardiac alpha tropomyosin, apolipoprotein A-I and albumin.

Conclusion

Down-regulated energy metabolism disorder mediated by NADH respiratory chain and myocardial injury may be the pathogenesis of myocardial ischemia with qi deficiency and blood stasis syndrome. These proteins may be the potential diagnostic marker(s) for qi deficiency and blood stasis syndrome, finally provided new clues for new therapeutic drug target of Chinese medicine

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Authors and Affiliations

  1. Beijing University of Chinese Medicine, Beijing, 100029, China

    Yong Wang  (王 勇), Wen-jing Chuo  (啜文静), Chun Li  (李 春), Shu-zhen Guo  (郭淑贞), Jian-xin Chen  (陈建新), Jun-da Yu  (余俊达) & Wei Wang  (王 伟)

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  1. Yong Wang  (王 勇)
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  2. Wen-jing Chuo  (啜文静)
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  3. Chun Li  (李 春)
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  4. Shu-zhen Guo  (郭淑贞)
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  5. Jian-xin Chen  (陈建新)
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  6. Jun-da Yu  (余俊达)
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  7. Wei Wang  (王 伟)
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Corresponding author

Correspondence to Wei Wang  (王 伟).

Additional information

Supported by the Creation for Significant New Drugs (2012ZX09103-201-011), National Science and Technology Pillar Program (No. 2012BAI29B07), and Project of Beijing University of Chinese Medicine (No. 2011-JYBZZ-JS055)

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Wang, Y., Chuo, Wj., Li, C. et al. Energy metabolism disorder and myocardial injury in chronic myocardial ischemia with Qi deficiency and blood stasis syndrome based on 2-DE proteomics. Chin. J. Integr. Med. 19, 616–620 (2013). https://doi.org/10.1007/s11655-012-1230-8

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  • DOI: https://doi.org/10.1007/s11655-012-1230-8

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