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Antimicrobial activity of the methanolic extract and compounds from the stem bark of Drypetes tessmanniana

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Abstract

Objective

To evaluate the antimicrobial activity of the methanol extract from the stem bark of Drypetes tessmanniana, fractions (DTB1-5) as well as compounds [friedelin (2), 3,7-dioxofriedelane (3), 3,15-dioxofriedelane (4), 3β- O-(E)-3,5-dihydroxycinnamoyl-11-oxo-olean-12-ene (6), and 3β,6α-dihydroxylup-20(29)-ene (7).

Methods

Agar disc diffusion was used to determine the sensitivity of the above samples, whilst the microdilution method was used for the determination of the minimal inhibitory concentration (MIC) and the minimal microbicidal concentrations (MMC).

Results

The diffusion test showed that the crude extract was able to prevent the growth of all tested organisms. All other samples showed selective activity. The inhibitory effect of the fraction DTB2 was noted on 63.7%, that of DTB1 and DBT3 on 54.6%, whilst DTB4 and DTB5 were active on 9.1% of the 11 tested organisms. The tested compounds prevented the growth of 81.8% of the tested microbial species for compounds 3 and 4, 36.7% for compound 6, and 18.2% for compound 7. The results of the MIC determinations indicated perceptible values for DTB and compound 4 on 81.8% of the tested organisms. For other samples, MICs were detected on 0–63.7%. The lowest MIC value (78.12 μg/mL) for the crude extract and fractions (DTB2) was observed on M. audouinii. The corresponding value for isolated compounds (156.25 μg/mL) was noted with compounds 3 on S. faecalis and 4 on M. audouinii audouinii. The results of the MMC determination suggested that the microbicidal effect of most of the tested samples on the studied microorganisms could be expected.

Conclusion

The methanol extract from the stem bark of Drypetes. tessmanniana (Euphorbiaceae) as well as some of the isolated compounds might be potential sources of new antimicrobial drugs.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon

    Victor Kuete

  2. Department of Organic Chemistry, Faculty of Science, University of Yaoundé I., P.O. Box 812, Yaoundé, Cameroon

    Marlise D. J. Dongfack & Jean Wandji

  3. Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon

    Armelle T. Mbaveng

  4. Laboratoire de Pharmacognosie de l’Université Paris Descartes, UMR/CNRS No 8638, Faculté des Sciences Biologiques et Pharmaceutiques, 4-Avenue de l’Observatoire, 75006, Paris, France

    Marlise D. J. Dongfack, Marie-Christine Lallemand, Hanh T. Van-Dufat & François Tillequin

  5. Department of Chemistry, University of Douala, Faculty of Science, P.O. Box 24157, Douala, Cameroon

    Jean-Duplex Wansi

  6. Laboratoire de Pharmacognosie, UFR de Médecine Pharmacie Rouen, 22-Bld Gambetta, 76183, Rouen Cedex 1, France

    Elisabeth Seguin

Authors
  1. Victor Kuete
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  2. Marlise D. J. Dongfack
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  3. Armelle T. Mbaveng
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  4. Marie-Christine Lallemand
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  5. Hanh T. Van-Dufat
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  6. Jean-Duplex Wansi
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  7. Elisabeth Seguin
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  8. François Tillequin
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  9. Jean Wandji
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Corresponding author

Correspondence to Victor Kuete.

Additional information

Supported by International Foundation for Science (No. IFS/2624-3F).

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Kuete, V., Dongfack, M.D.J., Mbaveng, A.T. et al. Antimicrobial activity of the methanolic extract and compounds from the stem bark of Drypetes tessmanniana . Chin. J. Integr. Med. 16, 337–343 (2010). https://doi.org/10.1007/s11655-010-0527-8

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  • DOI: https://doi.org/10.1007/s11655-010-0527-8

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