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Primary culture of colonocytes in rotating bioreactor

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References

  1. Booth, C.; Evans, G. S.; Potten, C. S. Growth factor regulation of proliferation in primary cultures of small intestinal epithelium. In Vitro Cell. Dev. Biol. 31:234–243; 1995.

    Article  CAS  Google Scholar 

  2. Chang, S. T.; Yung, B. Y. M. Potentiation of sodium butyrate-induced apoptosis by vanadate in human promyelocytic leukemia cell line HL-60. Biochem. Biophys. Res. Comm. 221:594–601; 1996.

    Article  PubMed  CAS  Google Scholar 

  3. Evans, G. S.; Flint, N.; Somers, A. S., et al. The development of a method for the preparation of rat intestinal epithelial cell primary cultures. J. Cell Sci. 101:219–231; 1992.

    PubMed  Google Scholar 

  4. Freed, L. E.; Vunjack-Novakovic, G. Microgravity tissue engineering. In Vitro Cell. Dev. Biol. 33A:381–385; 1997.

    Article  Google Scholar 

  5. Frisch, S. M.; Francis, H. Disruption of epithelial cell-matrix interactions induces apoptosis. J. Cell Biol. 124:619–626; 1994.

    Article  PubMed  CAS  Google Scholar 

  6. Giannasca, K. T.; Giannasca, P. J.; Neutra, M. R. Adherence of Salmonella typhimurium to Caco-2 cells: identification of a glycoconjugate receptor. Infect. Immun. 64:135–145; 1996.

    PubMed  CAS  Google Scholar 

  7. Goodwin, T. J.; Schroeder, W. F.; Wolf, D. A., et al. Rotating-wall vessel coculture of small intestine as a prelude to tissue modeling: aspects of simulated microgravity (43525). Proc. Soc. Exp. Biol. Med. 202:181–192; 1993.

    PubMed  CAS  Google Scholar 

  8. Huyer, G.; Liu, S.; Kelly, J., et al. Mechanism of inhibition of protein-tyrosine phosphatases by vanadate and pervanadate. J. Biol. Chem. 272:843–851; 1997.

    Article  PubMed  CAS  Google Scholar 

  9. Kaeffer, B.; Bénard, C.; Blottière, H. M., et al. Treatment of rat proximal and distal colonic cells with sodium orthovanadate enhances their adhesion and survival in primary culture. Cell Biol. Int. 21:303–314; 1997.

    Article  PubMed  CAS  Google Scholar 

  10. Konishi, H.; Steinbach, G.; Hittelman, W. N., et al. Cell kinetic analysis of intact rat colonic crypts by confocal microscopy and immunofluorescence. Gastroenterology 111:1493–1500; 1996.

    Article  PubMed  CAS  Google Scholar 

  11. Meredith, J. E.; Fazelik, B.; Schwartz, M. A. The extracellular matrix as a cell survival factor. Mol. Biol. Cell 4:953–961; 1993.

    PubMed  CAS  Google Scholar 

  12. Paulus, U.; Loeffler, M.; Zeidler, J., et al. The differentiation and lineage development of goblet cells in the murine small intestinal crypt: experimental and modelling studies. J. Cell Sci. 106:473–484; 1993.

    PubMed  Google Scholar 

  13. Pérez-Tomás, R.; Culleré, X.; Díaz, C. Immunohistochemical localization of transforming growth factor a in the developing rat colon. Gastroenterology 104:789–795; 1993.

    PubMed  Google Scholar 

  14. Perreault, N.; Beaulieu, J.-F. Use of the dissociating enzyme thermolysin to generate viable human normal intestinal epithelial cell cultures. Exp. Cell Res. 224:354–364; 1996.

    Article  PubMed  CAS  Google Scholar 

  15. Quaroni, A.; Beaulieu, J.-F. Cell dynamics and differentiation of conditionally immortalized human intestinal epithelial cells. Gastroenterology 113:1198–1213; 1997.

    Article  PubMed  CAS  Google Scholar 

  16. Scharma, R.; Schumacher, U. The influence of diets and gut microflora on lectin binding patterns of intestinal mucins in rats. Lab. Invest. 73:558–564; 1995.

    Google Scholar 

  17. Sörby, M.; Östman, A. Protein-tyrosine phosphatase-mediated decrease of epidermal growth factor and platelet-derived growth factor receptor tyrosine phosphorylation in high cell density cultures. J. Biol. Chem. 271:10963–10966; 1996.

    Article  PubMed  Google Scholar 

  18. Uribe, J. M.; Barrett, K. E. Nonmitogenic actions of growth factors: an integrated view of their role in intestinal physiology and pathophysiology. Gastroenterology 112:255–268; 1997.

    PubMed  CAS  Google Scholar 

  19. Watson, A. J. M. Necrosis and apoptosis in the gastrointestinal tract. Gut 37:165–167; 1995.

    PubMed  CAS  Google Scholar 

  20. Yang, C.; Chang, J.; Gorospe, M., et al. Protein tyrosine phosphatase regulation of endothelial cell apoptosis and differentiation. Cell Growth Differ. 7:161–171; 1996.

    PubMed  CAS  Google Scholar 

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Kaeffer, B., Briollais, S. Primary culture of colonocytes in rotating bioreactor. In Vitro Cell.Dev.Biol.-Animal 34, 622–625 (1998). https://doi.org/10.1007/s11626-996-0008-8

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  • DOI: https://doi.org/10.1007/s11626-996-0008-8

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