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Data Availability
The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.
Abbreviations
- CRISPR :
-
Clustered regularly interspaced short palindromic repeats
- crRNA :
-
CRISPR ribonucleic acid
- DGAT1 :
-
Diacylglycerol O-acyltransferase 1
- gRNA :
-
Guide ribonucleic acid
- MAC-T :
-
Primary bovine mammary alveolar cells immortalised by stable transfection with SV-40 large T-antigen
- SpCas9 :
-
(CRISPR)-associated S. pyogenes nuclease Cas9
- tracrRNA :
-
Trans-activating CRISPR ribonucleic acid
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Trevarton, A.J., Mears, E.R., Moody, J. et al. Development of SpCas9-expressing bovine mammary epithelial cell lines. In Vitro Cell.Dev.Biol.-Animal 59, 163–165 (2023). https://doi.org/10.1007/s11626-023-00756-4
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DOI: https://doi.org/10.1007/s11626-023-00756-4