Abstract
The diversity and extent of signaling functions of nitric oxide (NO) in cell physiology as well as its presence and influence as a common component of ambient air pollution and tobacco smoke are gaining increasing research attention relative to both health and disease. While cellular NO production is typically associated with inflammatory cells and processes, the airway epithelium particularly of the paranasal sinuses, has been documented to be a rich source of excreted NO. Inasmuch as excreted NO derives from both mucosal and inflammatory cell sources, distinguishing the individual contribution of these compartments to total excreted cellular NO is potentially problematic. We simulated an inflammatory mucosal environment by stimulating human nasal epithelial cultures with interleukin-13 (IL-13), a mediator produced by eosinophils in asthma, allergic rhinitis, and sinusitis. While a consistent baseline of NO excretion in control cultures was documented, widely variable individual responses to IL-13 exposure were observed in companion cultures maintained under identical conditions and tested at the same time. These studies suggest that cellular NO excretion by the healthy epithelial mucosa is subject to considerable individual variability and may be significantly elevated among some individuals in the presence of IL-13 stimulation.
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Acknowledgements
We thank Ms. Sally Ivins for coordination of subject recruitment.
Funding
This study was supported by grant numbers CIA123015 and CIA123009 to J. L. Carson and I. Jaspers respectively from the Flight Attendant Medical Research Institute. H. Zhou was supported by the National Institute of Environmental Health Sciences of the National Institutes of Health under award number P30ES010126.
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J. L. Carson and M. J. Hazucha are partners in Cytometric Sciences LLC. The authors declare that the funding sources and business disclosures had no role in the design of the study and collection, analysis, and interpretation of data and in the composition of the manuscript.
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Editor: Tetsuji Okamoto
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Carson, J.L., Hernandez, M., Jaspers, I. et al. Interleukin-13 stimulates production of nitric oxide in cultured human nasal epithelium. In Vitro Cell.Dev.Biol.-Animal 54, 200–204 (2018). https://doi.org/10.1007/s11626-018-0233-y
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DOI: https://doi.org/10.1007/s11626-018-0233-y