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Establishment and characterization of a continuous cell line from thymus of striped snakehead, Channa striatus (Bloch 1793)

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Abstract

The establishment and characterization of a continuous cell line from the thymus of air-breathing fish Channa striatus are described. The cell line, designated C. striatus thymus (CST), has been subcultured over 71 times and shows optimal growth at 28°C in Leibovitz’s-15 (L-15) medium supplemented with 20% fetal bovine serum. The CST cells exhibited low plating efficiency which improved with increase in seeding density. The karyotype analysis revealed that CST cells have a normal diploid karyotype with 2n = 40. Partial amplification and sequencing of two mitochondrial genes, viz. 16S ribosomal RNA (rRNA) and cytochrome oxidase I, confirmed that the cell line originated from C. striatus. CST cells were successfully transfected indicating their potential application for expression of recombinant proteins. In immunocytochemical staining, CST cells showed characteristics of epithelial cells. These cells were sensitive to extracellular products of Vibrio cholerae MTCC 3904 as well as to heavy metal mercuric chloride. The CST cell line would be a useful tool in functional genomic studies such as RNA interference and gene knockout as well as for cytotoxicity studies.

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Acknowledgments

The authors are thankful to Dr. S. Ayyappan, Secretary, DARE and Director-General, ICAR and Dr. B. Meenakumari, DDG (Fy) ICAR, New Delhi, India, for their support, encouragement, and guidance.

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Authors and Affiliations

  1. National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow, 226 002, Uttar Pradesh, India

    Neeraj Sood, D. K. Chaudhary, P. K. Pradhan, D. K. Verma, B. Kushwaha, P. Punia & J. K. Jena

  2. Peninsular and Marine Fish Genetic Resources Centre, National Bureau of Fish Genetic Resources, CMFRI Campus, P.O. Number 1603, Kochi, 682 018, Kerala, India

    T. Raja Swaminathan

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  1. Neeraj Sood
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  2. D. K. Chaudhary
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Correspondence to Neeraj Sood.

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Sood, N., Chaudhary, D.K., Pradhan, P.K. et al. Establishment and characterization of a continuous cell line from thymus of striped snakehead, Channa striatus (Bloch 1793). In Vitro Cell.Dev.Biol.-Animal 51, 787–796 (2015). https://doi.org/10.1007/s11626-015-9891-1

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