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Phospholipid hydroperoxide glutathione peroxidase gene is regulated via an estrogen and estrogen receptor signaling in cultured mouse fetuses

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Abstract

Although it has been suggested that the transcription of phospholipid hydroperoxide glutathione peroxidase (PHGPx), an essential antioxidant selenoenzyme, may be affected by the estrogen state in mammals, the direct mechanism underlying the regulation of the PHGPx gene by estrogens in mammalian tissues remains to be clearly elucidated. In this study, we evaluated the expression of the PHGPx mRNA in cultured mouse fetuses (embryonic days 8.5–10.5) exposed to 17β-estradiol (E2; 0.1, 1, 10, 100, and 1,000 ng/ml); estrogen receptor (ER) agonists [propyl pyrazole triol (PPT, an ERα-selective ligand, 1 μl/ml) and diarylpropionitrile (DPN, an ERβ-selective ligand, 1 μl/ml)]; and/or ER antagonist [ICI 182,780 (ICI, 1 μl/ml)] using a whole embryo culture system. E2-alone treatment significantly stimulated the expressions of both ERα and ERβ mRNAs in all the cultured fetuses (p < 0.05), although the ERβ mRNA levels were higher than ERα mRNA. PHGPx mRNA expression was significantly increased in all the fetuses treated with E2 (1–1,000 ng/ml), PPT, and DPN (p < 0.05). Furthermore, pretreatment with ICI completely blocked the E2-induced PHGPx mRNA expression in the fetuses. In addition, the mRNA levels of cytosolic GPx, the other intracellular antioxidant selenoenzyme, did not differ significantly from the controls by an exposure to those agents. These results suggest that the PHGPx gene is regulated via an estrogen and ER signal pathway in the cultured mouse fetus.

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Acknowledgments

This work was supported by Priority Research Centers Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0029709) and Korea Research Foundation Grant funded by the Korean Government (KRF-2008-357-E00069).

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Authors and Affiliations

  1. College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju, 361-763, South Korea

    Ki Youn Jung, Jung-Min Yon, Beom Jun Lee, Young Won Yun & Sang-Yoon Nam

  2. Laboratory of Mouse Molecular Genetics, Department of Biochemistry, College of Science, Yonsei University, Seoul, 120-749, South Korea

    In-Jeoung Baek

  3. CKD Research Institute, Chong Kun Dang Pharm, Chonan P.O. Box 74, Chonan, 330-600, South Korea

    Se-Ra Lee

  4. Laboratory of Veterinary Anatomy, College of Veterinary Medicine, Chungbuk National University, Cheongju, 361-763, South Korea

    Sang-Yoon Nam

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  1. In-Jeoung Baek
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  2. Ki Youn Jung
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  3. Jung-Min Yon
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  4. Se-Ra Lee
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  6. Young Won Yun
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Correspondence to Sang-Yoon Nam.

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Editor: T. Okamoto

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Baek, IJ., Jung, K.Y., Yon, JM. et al. Phospholipid hydroperoxide glutathione peroxidase gene is regulated via an estrogen and estrogen receptor signaling in cultured mouse fetuses. In Vitro Cell.Dev.Biol.-Animal 47, 535–540 (2011). https://doi.org/10.1007/s11626-011-9438-z

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  • DOI: https://doi.org/10.1007/s11626-011-9438-z

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