Abstract
The aim of this work was the establishment and characterization of porcine aortic endothelial cell cultures with prolonged lifespan. Endothelial cells where isolated from porcine thoracic aorta and established in primary culture; after sub cultivation, the cells showed typical morphology of endothelial phenotype with cobblestone appearance and growth in monolayer; they were positive against anti-CD31 and anti-CD54 immunostaining and Ac-LDL-Dil uptake. The cells were able to migrate in culture and showed a normal growth curve. The phenotype of our in vitro model of endothelial cells was stable through subcultivation; so, it should be a valuable tool for diverse studies of the endothelial response against physiological and pathological stimulus, particularly it could be useful to study interactions of endothelium with pathogenic bacteria causing diseases in pigs, as they are difficult to study in vivo.
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Editor: J. Denry Sato
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The animal samples were taken out of pigs from slaughterhouse with National Quality Control Certification (TIF, Federal Inspection Type). Therefore, the animals care standards and sanitary procedures were done according to Federal Mexican Regulations. The tissue harvesting was also done according to Mexican Regulations.
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Burciaga-Nava, J.A., Reyes-Romero, M.A., Avelar-González, F.J. et al. Establishment and characterization of porcine aortic endothelial cell cultures with prolonged replicative lifespan by a non-enzymatic method. In Vitro Cell.Dev.Biol.-Animal 45, 15–18 (2009). https://doi.org/10.1007/s11626-008-9146-5
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DOI: https://doi.org/10.1007/s11626-008-9146-5