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Mycobacterium avium–intracellulare contamination of mammalian cell cultures

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Abstract

Mycobacterium avium contamination has been described as a putative contaminant of nonphagocytic mammalian cells. Screening of numerous cultured nonphagocytic mammalian cell lines revealed the presence of intracellular bacteria that were identified as M. avium–intracellulare. An extensive and critical analysis of the origin of infection, of cure protocols, and of biological manifestations in M. avium-infected cells is presented. As no tremendous visible alteration of turbidity or pH of cell culture media, and no morphological change occurred in most M. avium-infected cell cultures, detection of an infection by these bacteria is rather difficult. Recommendations are given for treatment of irreplaceable cultures and prevention of mycobacterial contamination in a tissue culture facility.

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  1. EA3430 ULP/CNRS, IRCAD, Hôpitaux Universitaires de Strasbourg BP 426, 67091, Strasbourg Cedex, France

    I. H. Lelong-Rebel & G. Rebel

  2. Institut de Bactériologie de la Faculté de Médecine, Université Louis Pasteur & Hôpitaux Universitaires de Strasbourg – 3, rue Koeberle, 67000, Strasbourg, France

    Y. Piemont

  3. Institut d’Histologie de la Faculté de Médecine, Université Louis Pasteur & Hôpitaux Universitaires de Strasbourg – 3, rue Koeberle, 67000, Strasbourg, France

    M. Fabre

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  1. I. H. Lelong-Rebel
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Lelong-Rebel, I.H., Piemont, Y., Fabre, M. et al. Mycobacterium avium–intracellulare contamination of mammalian cell cultures. In Vitro Cell.Dev.Biol.-Animal 45, 75–90 (2009). https://doi.org/10.1007/s11626-008-9143-8

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