Abstract
Cell immortalization technology based on gene transfer has been successfully used to generate cell lines from a wide variety of cell types. The inability to stably introduce and express foreign genes has hampered application of this strategy in shrimp cells. We report here the use of replication-defective pantropic retrovirus to achieve a novel immortalization vector in which simian virus 40 large T antigen (SV40T) gene is expressed from Moloney murine leukemia virus (MoMLV) promoter. Data confirmed the presence of transferred SV40T gene and its stable mRNA expression in transduced lymphoid cells of Penaeus chinensis. The transduced cells showed a higher growth rate and a longer replication life-span compared with their untransduced counterparts. These results indicate the pantropic retrovirus-based immortalization-inducing gene delivery system is a potential tool for establishing cell lines from shrimp.
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Acknowledgements
We thank Dr. X.W. Wang for providing plasmid pSV3neo, Prof. J.X. Chen and Dr. J. Li for their assistance in the experiments. We also thank Dr. M. Kusakabe of the University of Tokyo for helpful comments on the manuscript. This work was supported by grant (NSFC30400334) from the National Natural Science Foundation of China.
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Editor: J. Denry Sato
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Hu, Gb., Wang, D., Wang, Ch. et al. A novel immortalization vector for the establishment of penaeid shrimp cell lines. In Vitro Cell.Dev.Biol.-Animal 44, 51–56 (2008). https://doi.org/10.1007/s11626-007-9076-7
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DOI: https://doi.org/10.1007/s11626-007-9076-7