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Danshen modulates Nrf2-mediated signaling pathway in cisplatin-induced renal injury

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Summary

Danshen, an efficacious agent for cardiovascular diseases, has been found to play an essential role in kidney injury. In the present study, the effect of Danshen on cisplatin-induced renal dysfunction was investigated in a mouse model. Danshen was administered to mice at a dose of 3 g/kg 4 days before and 3 days after cisplatin treatment. A single intraperitoneal injection of 20 mg/kg cisplatin was used to induce nephrotoxicity. The mice were sacrificed 72 h after cisplatin intoxication. Biochemical parameters including serum creatinine and blood urea nitrogen were analyzed. Histopathological changes of kidney tissues were detected using HE staining. Antioxidant enzymes (GSH-Px and SOD) and peroxidative product (MDA) were detected. Protein expressions of Nrf2 and its target genes including HO-1 and NQO1 were measured by Western blotting. The results showed that pretreatment with Danshen significantly reduced serum creatinine and blood urea nitrogen in the cisplatin-treated mice. Histopathological examination showed that Danshen mitigated the renal damage induced by cisplatin. Moreover, Danshen restored the activities of antioxidant enzymes (GSH-Px and SOD) and normalized the MDA contents in renal tissues. Western blotting revealed that Danshen enhanced the expressions of Nrf2 and its target genes in cisplatin-exposed mice. It was suggested that Danshen protects against the cisplatin-induced renal impairment in the mice, which is potentially associated with the upregulation of Nrf2-mediated signaling pathway.

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Authors and Affiliations

  1. Department of Pharmacy, the Second Xiangya Hospital, Central South University, Changsha, 410011, China

    Si-si Cao  (曹思思), Miao Yan  (颜 苗), Zhen-yan Hou  (侯振彦), Ying Chen  (陈 莹), Ping-fei Fang  (方平飞) & Bi-kui Zhang  (张毕奎)

  2. School of Pharmaceutical Sciences, Central South University, Changsha, 410013, China

    Si-si Cao  (曹思思) & Ying Chen  (陈 莹)

  3. Department of Nephrology, the Second Xiangya Hospital, Central South University, Changsha, 410011, China

    Yun-sheng Jiang  (蒋云生)

  4. Institute of Basic Theories of Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing, 100700, China

    Xin-rong Fan  (樊新荣)

Authors
  1. Si-si Cao  (曹思思)
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  2. Miao Yan  (颜 苗)
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  3. Zhen-yan Hou  (侯振彦)
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  4. Ying Chen  (陈 莹)
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  5. Yun-sheng Jiang  (蒋云生)
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  6. Xin-rong Fan  (樊新荣)
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  7. Ping-fei Fang  (方平飞)
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  8. Bi-kui Zhang  (张毕奎)
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Corresponding author

Correspondence to Bi-kui Zhang  (张毕奎).

Additional information

This project was supported by grants from the National Natural Science Foundation of China (Nos. 81202985 and 81573686).

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Cao, Ss., Yan, M., Hou, Zy. et al. Danshen modulates Nrf2-mediated signaling pathway in cisplatin-induced renal injury. CURR MED SCI 37, 761–765 (2017). https://doi.org/10.1007/s11596-017-1801-1

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  • DOI: https://doi.org/10.1007/s11596-017-1801-1

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