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P2X3, but not P2X1, receptors mediate ATP-activated current in neurons innervating tooth-pulp

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Summary

We developed a method that allows us to label nociceptive neurons innervating tooth-pulp in rat trigeminal ganglion neurons using a retrograde fluorescence-tracing method, to record ATP-activated current in freshly isolated fluorescence-labeled neurons and to conduct single cell immunohistochemical staining for P2X1 and P2X3 subunits in the same neuron. Three types of ATP-activated current in these neurons (F, I and S) were recorded. The cells exhibiting the type F current mainly showed positive staining for P2X3, but negative staining for P2X1. The results provide direct and convincing evidence at the level of single native nociceptive neurons for correlation of the characteristics of ATP-activated currents with their composition of P2X1 and P2X3 subunits and cell size. The results also suggest that the P2X3, but not P2X1, is the main subunit that mediates the fast ATP-activated current in nociceptive neurons.

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Authors and Affiliations

  1. Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan, 430056, China

    Yu-wei Liu  (刘宇炜), Xiang Tian  (田 香), Lin Chen  (陈 琳), Yu-xiang Wu  (吴钰祥) & Chao-ying Li  (李超英)

  2. School of Medicine, Jianghan University, Wuhan, 430056, China

    Yu-wei Liu  (刘宇炜), Xiao-qing Chen  (陈晓青), Dan Huang  (黄 丹), Hui-ling Yi  (易卉玲) & Chu-li Yi  (易初丽)

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  1. Yu-wei Liu  (刘宇炜)
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  2. Xiao-qing Chen  (陈晓青)
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  3. Xiang Tian  (田 香)
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  4. Lin Chen  (陈 琳)
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  5. Yu-xiang Wu  (吴钰祥)
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  6. Dan Huang  (黄 丹)
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  7. Hui-ling Yi  (易卉玲)
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  8. Chu-li Yi  (易初丽)
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  9. Chao-ying Li  (李超英)
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Corresponding author

Correspondence to Chao-ying Li  (李超英).

Additional information

These authors contributed equally to this work.

This project was supported by grants from the National Natural Science Foundation of China (No. 30970927), the Natural Science Foundation of Hubei Province, China (No. 2008CDA053) and the Wuhan Science and Technology Foundation (Nos. 200970634270, 201250499145-27).

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Liu, Yw., Chen, Xq., Tian, X. et al. P2X3, but not P2X1, receptors mediate ATP-activated current in neurons innervating tooth-pulp. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 33, 423–426 (2013). https://doi.org/10.1007/s11596-013-1135-6

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  • DOI: https://doi.org/10.1007/s11596-013-1135-6

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