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Effect of renin angiotensin system blockade on the islet microvessel density of diabetic rats and its relationship with islet function

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To investigate the effects of rennin angiotensin system blockade on the microvessel density in islets of diabetic rats and its relationship with islet function, diabetes model was created by feeding of high-caloric laboratory chow plus intraperitoneal injection of a small dose of streptozotocin (30 mg/kg). After 8 weeks intervention with perindopril (AE, n=10) or valsartan (AR, n=10), the islet function of the animals was evaluated by intravenous insulin release test (IVIRT). The pancreases were immunohistochemically stained to analyze the content of insulin and vascular endothelial growth factor (VEGF) in the islets. The microvessel density (MVD) of islets was detected by counting CD34 positive cells. The hypoxia inducible factor (HIF)-1α mRNA expression in the islets was detected by RT-PCR. Compared with normal control group (NC, n=10), the area under the curve for insulin from 0 to 30 min (AUCI0–30) of diabetes group (DM, n=8) was decreased by 66.3%; the insulin relative concentration (IRC) of βcell was decreased significantly; the relative content of VEGF was increased obviously [(−4.21±0.13) vs (−4.06±0.29)]; MVD in islets was decreased by 71.4%; the relative expression of HIF-1α mRNA was increased by 1.19 times (all P<0.01). Compared with DM group, the AUCI0-30 of AE and AR group was increased by 44.6% and 34.9% respectively; IRC was also increased significantly; the relative content of VEGF was decreased by 21.2% and 21.7% respectively; MVD was increased by 62.5% and 75.0% respectively; the relative expression of HIF-1α was decreased by 27.2% and 29.0% respectively (all P<0.01 or P<0.05). There were no significant differences in the said indexes between group AE and AR. It is concluded that the blockade of RAS may ameliorate islets function of diabetic rats by increasing the MVD in islets.

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Authors and Affiliations

  1. Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China

    Xin Li  (李 新), Li Yuan  (袁 莉), Guoling Xu  (徐国玲), Cuijuan Qi  (齐翠娟), Jin Li  (李 进), Hailing Li  (李海玲) & Suosuo Cheng  (程梭梭)

  2. Department of Endocrinology, Zhongnan Hospital, Wuhan University, Wuhan, 430071, China

    Xin Li  (李 新)

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  1. Xin Li  (李 新)
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  2. Li Yuan  (袁 莉)
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  3. Guoling Xu  (徐国玲)
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  4. Cuijuan Qi  (齐翠娟)
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  5. Jin Li  (李 进)
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  6. Hailing Li  (李海玲)
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  7. Suosuo Cheng  (程梭梭)
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Additional information

This project was supported by a grant from the Provincial Natural Sciences Foundation of Hubei, China (No. 2005ABA158).

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Li, X., Yuan, L., Xu, G. et al. Effect of renin angiotensin system blockade on the islet microvessel density of diabetic rats and its relationship with islet function. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 29, 684–688 (2009). https://doi.org/10.1007/s11596-009-0602-6

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  • DOI: https://doi.org/10.1007/s11596-009-0602-6

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