Abstract
Species of Penicillium sect. Exilicaulis are responsible for the production of antimicrobial compounds, but they can also produce genotoxic compounds that affect commercial mushroom production or cause disease in immunosuppressed animals. In the present study, based on morphological characters such as the length of the conidia, phialides, and stipes, as well as sequence analysis of the ITS region and partial sequence of CAM, and RPB2 loci, we describe Penicillium amapaense as new fungal species and report for the first time the occurrence of Penicillium labrodorum in Brazil, both of which were isolated from sediments of the Amazon River. The isolates obtained in this study for each species were submitted to antibiosis assays against 12 phytopathogenic fungi that affect important agricultural crops in Brazil and showed inhibition of 11 out of 12 of them. The production of amylase, cellulase, and siderophore as well as phosphate solubilization was also detected. Metabolomic analysis indicates the ability of P. labrodorum and P. amapaense sp. nov. to produce polyketides such as known curvularins and anthraquinones. In addition to these, unknown compounds were also detected. These results indicate the biotechnological and agricultural potential of P. labradorum and P. amapaense increasing our knowledge on Penicillium biodiversity in the aquatic environment of the amazon biome.
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The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request
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Acknowledgements
The authors would like to thank the Independent Center for Analysis of Biomedical Phenomena (CMABio) and the Amazonas State University (UEA) for their support in the activities of Electron Microscopy. The authors are also very grateful for the contribution of Cristóvão Tertuliano de Almeida Lins who kindly sent us sediments from the Amazon River that resulted in the isolation of the new species P. amapaense.
Funding
This work was funded by the National Council for Scientific and Technological Development (CNPq grant 435705/2018-0), National Council for the Improvement of Higher Education Personnel (CAPES- grant number 88881.200469/2018-01 Procad AmazonMicro and Grant N° 88887.510218-2020-00 CAPES-Amazônia-Legal), FAPEAM (Amazonas State Research Support Foundation grants: Amazonas Estratégico 062.01311/2018, PAMEQ Edital 2018, PROSPAM-Edital 2021and Areas Prioritárias-Edital 2021 for G.F.S. and Amazonas Estratégico 062.01303/2018 for H.H.F.K.).
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The conceptualization and supervision were performed by Gilvan Ferreira da Silva; fungal acquisition were performed by Ingride Jarline S. da Silva and Rogério Eiji Hanada; taxonomic analysis were performed by Ingride Jarline S. da Silva and Thiago Fernandes Sousa; DNA extraction and sanger sequencing were performed by Gilvan Ferreira da Silva and Ingride Jarline S. da Silva; fungal fermentation and extraction were performed by Gleucinei dos Santos Castro; dual culture assays were performed by Ingride Jarline S. da Silva; enzymatic assays were performed by Cláudia Afras de Queiroz; LC-MS/MS analysis were performed by Gleucinei dos Santos Castro, Hector Henrique Ferreira Koolen, Lívia S. de Medeiros and Célio F. F. Angolini; statistical analysis were performed by Fernanda Fátima Caniato; writing and original draft preparation were performed by Ingrid Jarline S. da Silva, Thiago Fernandes Sousa, Hector Henrique Ferreira Koolen and Gilvan Ferreira da Silva; writing, review and editing were performed by all co-authors; project administration and funding acquisition were performed by Hector Henrique Ferreira Koolen and Gilvan Ferreira da Silva. All the authors contributed equally to the study conception and design. All the authors commented on the previous versions of the manuscript. All the authors read and approved the final manuscript.
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da Silva, I.J.S., Sousa, T.F., de Queiroz, C.A. et al. Penicillium amapaense sp. nov., section Exilicaulis, and new records of Penicillium labradorum in Brazil isolated from Amazon River sediments with potential applications in agriculture and biotechnology. Mycol Progress 22, 23 (2023). https://doi.org/10.1007/s11557-023-01868-7
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DOI: https://doi.org/10.1007/s11557-023-01868-7