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Sugarcane glycoproteins control dynamics of cytoskeleton during teliospore germination of Sporisorium scitamineum

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Abstract

Sporisorium scitamineum teliospores possess an organized cytoskeleton involved in important developmental and physiological processes. It has been described that microtubules appear to be fundamental for nucleus translocation during germination and hyphal growth, whereas actin polymerization is necessary for the formation of invaginations during teliospore displacement. Here, a global vision of the actin cytoskeleton organization throughout the life cycle of S. scitamineum cells is shown, providing evidence that a perfectly structured F-actin network is necessary to trigger smut pathogenicity. Moreover, although myosin presence in teliospores had been previously described, herein actin and myosin co-locations are demonstrated by confocal microscopy during both invaginations formation and germination. In turn, F-actin and microtubules (MTs) interact, jointly participating in the establishment of cell polarity. The resistant sugarcane cultivar Mayari 55-14 produces high molecular mass glycoproteins (HMMG) that differently affect F-actin organization at different stages of fungal development. HMMG first supported F-actin to induce the movement of teliospores towards the cytoagglutination points. At later stages of fungal development, HMMG disorganized F-actin which prevented the protrusion of germinative tube. A continuous exposure to HMMG provoked apoptosis in pathogenic, diploid cells and a delay in sporidia conjugation that could be crucial for plant resistance.

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Abbreviations

B:

Barbados

BSA:

Bovine serum albumin

DAPI:

4′,6-Diamidino-2-phenylindole dihydrochloride

HMMG:

High molecular mass glycoproteins

IgG:

Immunoglobulin G

Jas:

Jasplakinolide

Lat A:

Latrunculin A

MT:

Microtubules

My:

Mayarí

Noc:

Nocodazole

PBS:

Phosphate saline buffer

PVP:

Polyvinylpyrrolidone

TEM:

Transmission electron microscopy

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Funding

This work has been supported by a grant from the Complutense University (Spain) UCM, GR3/14.910081.

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Authors and Affiliations

  1. Team of Intercellular Communication in Plant Symbiosis, Faculty of Biology, Complutense University, 12 José Antonio Novais Av., 28040, Madrid, Spain

    Elena Sánchez-Elordi, Carlos Vicente & María Estrella Legaz

  2. Department of Plant Cell Biology, Institute of Cellular and Molecular Botany (IZMB), University Bonn, Kirschallee 1, 53115, Bonn, Germany

    František Baluška

Authors
  1. Elena Sánchez-Elordi
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  2. František Baluška
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  3. Carlos Vicente
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  4. María Estrella Legaz
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Correspondence to María Estrella Legaz.

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Section Editor: Marc Stadler

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Sánchez-Elordi, E., Baluška, F., Vicente, C. et al. Sugarcane glycoproteins control dynamics of cytoskeleton during teliospore germination of Sporisorium scitamineum. Mycol Progress 18, 1121–1134 (2019). https://doi.org/10.1007/s11557-019-01510-5

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  • DOI: https://doi.org/10.1007/s11557-019-01510-5

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