Abstract
Correlation between lactic acid bacteria (LAB) survivability and physical properties of microcapsules is critical to revealing the protecting mechanism of microcapsules for LAB. In this paper, five formulae of microcapsules with increasing mechanical strength were chosen to encapsulate Lactobacillus acidophilus CGMCC1.2686 using the method of emulsification/internal gelation. Morphological and size characteristics showed that particle diameter of the five LAB microcapsules ranged from 263.4 to 404.6 μm, with a span factor from 0.87 to 1.12. The increased mechanical strength of the microcapsules was accompanied with increased viscoelasticity and structural compactness as observed by scanning electron microscopy. Most of the microspheres shrinked in simulated gastric juice (SGJ), whilst swelling in bile salts solution (BS). Regression analysis showed that cell viability in SGJ was positively correlated with the mechanical strength of microcapsules. However, increasing mechanical strength did not significantly improve cell survival in BS.
Similar content being viewed by others
References
J. Walter, Appl. Environ. Microbiol. 74(16), 4985–4996 (2008)
T. Mattila-Sandholm, P. Myllärinen, R. Crittenden, G. Mogensen, R. Fondén, M. Saarela, Int. Dairy J. 12(2–3), 173–182 (2002)
S. Sarkar, Br. Food J. 112(4), 329–349 (2010)
J. Burgain, C. Gaiani, M. Linder, J. Scher, J. Food Eng. 104(4), 467–483 (2011)
P. Gacesa, Carbohydr. Polym. 8(3), 161–182 (1988)
C.A. Hoesli, K. Raghuram, R.L.J. Kiang et al., Biotechnol. Bioeng. 108(2), 424–434 (2011)
W. Krasaekoopt, B. Bhandari, H. Deeth, Int. Dairy J. 13(1), 3–13 (2003)
D. Poncelet, B. Poncelet De Smet, C. Beaulieu, M.L. Huguet, A. Fournier, R.J. Neufeld, Appl. Microbiol. Biotechnol. 43(4), 644–650 (1995)
B.C. Larisch, D. Poncelet, C.P. Champagne, R.J. Neufeld, J. Microencapsul. 11(2), 189–195 (1994)
M.J. Martin, F. Lara-Villoslada, M.A. Ruiz, M.E. Morales, LWT Food Sci. Technol. 53(2), 480–486 (2013)
Q. Zou, J. Zhao, X. Liu et al., Int. J. Food Sci. Technol. 46(8), 1672–1678 (2011)
C.A. Hoesli, R.L.J. Kiang, D. Mocinecová et al., J. Biomed. Mater. Res. B 100B(4), 1017–1028 (2012)
A. Esquisabelr, M. Hernáandez, M. Igartuaa, R. Gascóan, B. Calvo, J.L. Pedraz, J. Microencapsul. 14(5), 627–638 (1997)
R. Börner, M. Aliaga, B. Mattiasson, Biotechnol. Lett. 35(3), 397–405 (2013)
E. Pales Espinosa, L. Barillé, B. Allam, J. Exp. Mar. Biol. Ecol. 343(1), 118–126 (2007)
X. Wang, K.-X. Zhu, H.-M. Zhou, Int. J. Mol. Sci. 12(5), 3042–3054 (2011)
Q. Liu, A.M. Rauth, X.Y. Wu, Int. J. Pharm 339(1–2), 148–156 (2007)
A.W.J. Chan, I. Mazeaud, T. Becker, R.J. Neufeld, Enzym Microb. Technol. 38(1–2), 265–272 (2006)
C.M. Silva, A.J. Ribeiro, D. Ferreira, F. Veiga, Eur. J. Pharm. Sci. 29(2), 148–159 (2006)
C.M. Silva, A.J. Ribeiro, I.V. Figueiredo, A.R. Gonçalves, F. Veiga, Int. J. Pharma 311(1–2), 1–10 (2006)
L. Baruch, M. Machluf, Biopolymers 82(6), 570–579 (2006)
O. Sandoval-Castilla, C. Lobato-Calleros, H.S. García-Galindo, J. Alvarez-Ramírez, E.J. Vernon-Carter, Food Res. Int. 43(1), 111–117 (2010)
U. Schuldt, D. Hunkeler, J. Microencapsul. 24(1), 1–10 (2007)
S. Cai, M. Zhao, Y. Fang, K. Nishinari, G.O. Phillips, F. Jiang, Food Hydrocoll. 39, 295–300 (2014)
J.C. De Man, M. Rogosa, M.E. Sharpe, J. Appl. Bacteriol. 23(1), 130–135 (1960)
A. Lefebvre, G.A. Matthews, E.C. Hislop, Application technology for crop protection (CAB, Wallingford, 1993), pp. 85–100
A.A. Guerra-Ordaz, G. González-Ortiz, R.M. La Ragione et al., Appl. Environ. Microbiol. 80(16), 4879–4886 (2014)
X.D. Liu, D.C. Bao, W.M. Xue et al., J. Appl. Polym. Sci. 87(5), 848–852 (2003)
Y. Sultana, S. Mall, D.P. Maurya, D. Kumar, M. Das, Pharm. Dev. Technol. 14(3), 321–331 (2009)
X. Li, X. Chen, J. Ocean Univ. China 8(1), 39–44 (2009)
G.A. Junter, F. Vinet, Chem. Eng. J. 145(3), 514–521 (2009)
J.M. Van Raamsdonk, P.L. Chang, J. Biomed. Mater. Res. 54(2), 264–271 (2001)
C.K. Kuo, P.X. Ma, Biomaterials 22(6), 511–521 (2001)
M.A. LeRoux, F. Guilak, L.A. Setton, J. Biomed. Mater. Res. 47(1), 46–53 (1999)
Y. Murata, K. Nakada, E. Miyamoto, S. Kawashima, S.-H. Seo, J. Control. Release 23(1), 21–26 (1993)
H. Song, W. Yu, M. Gao, X. Liu, X. Ma, Carbohydr. Polym. 96(1), 181–189 (2013)
V. Chandramouli, K. Kailasapathy, P. Peiris, M. Jones, J. Microbiol. Meth. 56(1), 27–35 (2004)
K.-Y. Lee, T.-R. Heo, Appl. Environ. Microbiol. 66(2), 869–873 (2000)
S. Mandal, A.K. Puniya, K. Singh, Int. Dairy J. 16(10), 1190–1195 (2006)
I. Trabelsi, W. Bejar, D. Ayadi et al., Int. J. Biol. Macromol. 61, 36–42 (2013)
L.-S. Chou, B. Weimer, J. Dairy Sci. 82(1), 23–31 (1999)
D. Provenzano, C.M. Lauriano, K.E. Klose, J. Bacteriol. 183(12), 3652–3662 (2001)
J.Y. Sung, E.A. Shaffer, J.W. Costerton, Dig. Dis. Sci. 38(11), 2104–2112 (1993)
Acknowledgments
The research was supported by National Natural Science Foundation of China (31322043, 31171751), Natural Science Foundation of Hubei Province (2012FFB00705, 2012FFA004), Projects from Hubei Provincial Department of Education (Q20141401, T201307), Scientific Research Foundation of Hubei University of Technology (BSQD12051), Program for New Century Excellent Talents in University (NCET-12-0710), and Key Project of Chinese Ministry of Education (212117).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Zhao, M., Qu, F., Cai, S. et al. Microencapsulation of Lactobacillus acidophilus CGMCC1.2686: Correlation Between Bacteria Survivability and Physical Properties of Microcapsules. Food Biophysics 10, 292–299 (2015). https://doi.org/10.1007/s11483-014-9389-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11483-014-9389-5