Skip to main content
SpringerLink
Log in

Genetic analysis and gene fine mapping of a rolling leaf mutant (rl 11(t) ) in rice (Oryza sativa L.)

  • Articles
  • Crop Genetics
  • Published:
Chinese Science Bulletin

Abstract

The exploration of new genes controlling rice leaf shape is an important foundation for rice functional genomics and plant architecture improvement. In the present study, we identified a rolling leaf mutant from indica variety Yuefeng B, named rl 11(t) , which exhibited reduced plant height, rolling and narrow leaves. Leaves in rl 11(t) mutant showed abnormal number and morphology of veins compared with those in wild type plants. In addition, rl 11(t) mutant was less sensitive to the inhibitory effect of auxin than the wild type. Genetic analysis suggested that the mutant was controlled by a single recessive gene. Gene rl 11(t) was initially mapped between SSR markers RM6089 and RM124 on chromosome 4. Thirty-two new STS markers around the rl 11(t) region were developed for fine mapping. A physical map encompassing the rl 11(t) locus was constructed and the target gene was finally delimited to a 31.6 kb window between STS4-25 and STS4-26 on BAC AL606645. This provides useful information for cloning of rl 11(t) gene.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Zhu D F, Lin X Q, Cao W X. Comparison of leaf photosynthetic characteristics among rice hybrids with different leaf rolling index (in Chinese). Acta Agron Sin, 2001, 27:329–333

    Google Scholar 

  2. Lang Y Z, Zhang, Z J, Gu, X Y, et al. Physiological and ecological effects of crimpy leaf character in rice (Oryza sativa L.) I. Leaf orientation, canopy structure and light distribution (in Chinese). Acta Agron Sin, 2004, 30:739–744

    Google Scholar 

  3. Lang Y Z, Zhang, Z J, Gu, X Y, et al. Physiological and ecological effects of crimpy leaf character in rice (Oryza sativa L.) II. Photosynthetic character, dry mass production and yield forming (in Chinese). Acta Agron Sin, 2004, 30:883–887

    Google Scholar 

  4. Yuan L P. Super-high yield hybrid rice breeding (in Chinese). Hybrid Rice, 1997, 12:1–6

    Google Scholar 

  5. Chen Z X, Pan X B, Hu J, et al. Relationship between rolling leaf and ideal plant type of rice (Oryza sativa L.) (in Chinese). J Jiangsu Agric Res, 2001, 22:88–91

    Google Scholar 

  6. Li S G, Ma Y Q, He P, et al. Genetic analysis and mapping the flag leaf rolling rice (Oryza sativa L.) (in Chinese). J Sichuan Agric Univ, 1998, 16:391–393

    Google Scholar 

  7. Shao, Y J, Chen Z X, Zhang Y F, et al. One major QTL mapping and physical map construction for rolling leaf in rice (in Chinese). Acta Genet Sin, 2005, 32:501–506

    Google Scholar 

  8. Gu X Y, Gu M H. Genetic analysis of a rolling leaf character in rice (in Chinese). Hereditas, 1995, 17:20–23

    Google Scholar 

  9. Shao Y J, Pan C H, Chen Z X, et al. Fine mapping of an incomplete recessive gene for leaf rolling in rice (Oryza sativa L.). Chinese Sci Bull, 2005, 50:2466–2472

    Google Scholar 

  10. Yan C J, Yan S, Zhang Z Q, et al. Genetic analysis and gene fine mapping for a rice novel mutant (rl 9(t)) with rolling leaf character. Chinese Sci Bull, 2006, 50:63–69

    Article  Google Scholar 

  11. Luo Z, Yang Z, Zhong B, et al. Genetic analysis and fine mapping of a dynamic rolled leaf gene, RL10(t), in rice (Oryza sativa L.). Genome, 2007, 50:811–817

    Article  Google Scholar 

  12. Yu D, Wu H B, Yang W T, et al. Genetic analysis and mapping of the unilateral rolled leaf trait of rice mutant B157 (in Chinese). Mol Plant Breeding, 2008, 6:220–226

    Google Scholar 

  13. Wang D K, Liu H Q, Li K L, et al. Genetic analysis and gene mapping of a narrow leaf mutant in rice (Oryza sativa L.). Chinese Sci Bull, 2009, 54:752–758

    Article  Google Scholar 

  14. Kong Y, Wang Z, Gu Y J, et al. Observations on the structure of Chlorophytum comosum roots under soil and water culture (in Chinese). Acta Horticult Sin, 2009, 36:533–538

    Google Scholar 

  15. Nelson T, Dengler N. Leaf vascular pattern formation. Plant Cell, 1997, 9:1121–1135

    Article  Google Scholar 

  16. Hobbie L, McGovern M, Hurwitz L R, et al. The axr6 mutants of Arabidopsis thaliana define a gene involved in auxin response and early development. Development, 2000, 127:23–32

    Google Scholar 

  17. Shi Z, Wang J, Wan X, et al. Over-expression of rice OsAGO7 gene induces upward curling of the leaf blade that enhanced erect-leaf habit. Planta, 2007, 226:99–108

    Article  Google Scholar 

  18. Yan S, Yan C J, Zeng X H, et al. ROLLED LEAF 9, encoding a GARP protein, regulates the leaf abaxial cell fate in rice. Plant Mol Biol, 2008, 68:239–250

    Article  Google Scholar 

  19. Zhang G H, Xu Q, Zhu X D, et al. SHALLOT-LIKE1 is a KANADI transcription factor that modulates rice leaf rolling by regulating leaf abaxial cell development. Plant Cell, 2009, 21:719–735

    Article  Google Scholar 

  20. Woo Y M, Park H J, Su’udi M, et al. Constitutively wilted 1, a member of the rice YUCCA gene family, is required for maintaining water homeostasis and an appropriate root to shoot ratio. Plant Mol Biol, 2007, 65:125–136

    Article  Google Scholar 

  21. Fujino K, Matsuda Y, Ozawa K, et al. NARROW LEAF 7 controls leaf shape mediated by auxin in rice. Mol Genet Genomics, 2008, 279:499–507

    Article  Google Scholar 

  22. Qi J, Qian Q, Bu Q, et al. Mutation of the rice Narrow leaf1 gene, which encodes a novel protein, affects vein patterning and polar auxin transport. Plant Physiol, 2008, 147:1947–1959

    Article  Google Scholar 

  23. Cheng Y, Dai X, Zhao Y. Auxin biosynthesis by the YUCCA flavin monooxygenases controls the formation of floral organs and vascular tissues in Arabidopsis. Genes Dev, 2006, 20:1790–1799

    Article  Google Scholar 

  24. Zhao Y, Christensen S K, Fankhauser C, et al. A role for flavin monooxygenase-like enzymes in auxin biosynthesis. Science, 2001, 291:306–309

    Article  Google Scholar 

  25. Cheng Y, Dai X, Zhao Y. Auxin synthesized by the YUCCA flavin monooxygenases is essential for embryogenesis and leaf formation in Arabidopsis. Plant Cell, 2007, 19:2430–2439

    Article  Google Scholar 

  26. Qin G, Gu H, Zhao Y, et al. An indole-3-acetic acid carboxyl methyltransferase regulates Arabidopsis leaf development. Plant Cell, 2005, 17:2693–2704

    Article  Google Scholar 

  27. Blakeslee J J, Peer W A, Murphy A S. Auxin transport. Curr Opin Plant Biol, 2005, 8:494–500

    Article  Google Scholar 

  28. Fukuda H. Signals that control plant vascular cell differentiation. Nat Rev Mol Cell Biol, 2004, 5:379–391

    Article  Google Scholar 

  29. Sauer M, Balla J, Luschnig C, et al. Canalization of auxin flow by Aux/IAA-ARF-dependent feedback regulation of PIN polarity. Genes Dev, 2006, 20:2902–2911

    Article  Google Scholar 

  30. Teale W D, Paponov I A, Palme K. Auxin in action: Signalling, transport and the control of plant growth and development. Nat Rev Mol Cell Biol, 2006, 7:847–859

    Article  Google Scholar 

  31. Galweiler L, Guan C, Muller A, et al. Regulation of polar auxin transport by AtPIN1 in Arabidopsis vascular tissue. Science, 1998, 282:2226–2230

    Article  Google Scholar 

  32. Mattsson J, Sung Z R, Berleth T. Responses of plant vascular systems to auxin transport inhibition. Development, 1999, 126:2979–2991

    Google Scholar 

  33. Okada K, Ueda J, Komaki M K, et al. Requirement of the auxin polar transport system in early stages of Arabidopsis floral bud formation. Plant Cell, 1991, 3:677–684

    Article  Google Scholar 

  34. Geisler M, Blakeslee J J, Bouchard R, et al. Cellular efflux of auxin catalyzed by the Arabidopsis MDR/PGP transporter AtPGP1. Plant J, 2005, 44:179–194

    Article  Google Scholar 

  35. Noh B, Bandyopadhyay A, Peer W A, et al. Enhanced gravi- and phototropism in plant mdr mutants mislocalizing the auxin efflux protein PIN1. Nature, 2003, 423:999–1002

    Article  Google Scholar 

  36. Noh B, Murphy A S, Spalding E P. Multidrug resistance-like genes of Arabidopsis required for auxin transport and auxin-mediated development. Plant Cell, 2001, 13:2441–2454

    Article  Google Scholar 

  37. Friml J, Yang X, Michniewicz M, et al. A PINOID-dependent binary switch in apical-basal PIN polar targeting directs auxin efflux. Science, 2004, 306:862–865

    Article  Google Scholar 

  38. Dai Y, Wang H, Li B, et al. Increased expression of MAP KINASE KINASE7 causes deficiency in polar auxin transport and leads to plant architectural abnormality in Arabidopsis. Plant Cell, 2006, 18:308–320

    Article  Google Scholar 

  39. Hamann T, Benkova E, Baurle I, et al. The Arabidopsis BODENLOS gene encodes an auxin response protein inhibiting MONOPTEROSmediated embryo patterning. Genes Dev, 2002, 16:1610–1615

    Article  Google Scholar 

  40. Hardtke C S, Berleth T. The Arabidopsis gene MONOPTEROS encodes a transcription factor mediating embryo axis formation and vascular development. EMBO J, 1998, 17:1405–1411

    Article  Google Scholar 

  41. Harper R M, Stowe-Evans E L, Luesse D R, et al. The NPH4 locus encodes the auxin response factor ARF7, a conditional regulator of differential growth in aerial Arabidopsis tissue. Plant Cell, 2000, 12:757–770

    Article  Google Scholar 

  42. Leyser H M, Pickett F B, Dharmasiri S, et al. Mutations in the AXR3 gene of Arabidopsis result in altered auxin response including ectopic expression from the SAUR-AC1 promoter. Plant J, 1996, 10:403–413

    Article  Google Scholar 

  43. Tian Q, Reed J W. Control of auxin-regulated root development by the Arabidopsis thaliana SHY2/IAA3 gene. Development, 1999, 126:711–721

    Google Scholar 

  44. Zgurski J M, Sharma R, Bolokoski D A, et al. Asymmetric auxin response precedes asymmetric growth and differentiation of asymmetric leaf1 and asymmetric leaf2 Arabidopsis leaves. Plant Cell, 2005, 17:77–91

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Jiangsu Key Laboratory for Crop Genetics and Physiology/Jiangsu Key Laboratory of Plant Functional Genomics, Yangzhou University, Yangzhou, 225009, China

    Yong Zhou, YunXia Fang, JinYan Zhu, ShengQiang Li, Fei Gu, MingHong Gu & GuoHua Liang

Authors
  1. Yong Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. YunXia Fang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. JinYan Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. ShengQiang Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Fei Gu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. MingHong Gu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. GuoHua Liang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to GuoHua Liang.

Additional information

These authors contributed equally to this work

About this article

Cite this article

Zhou, Y., Fang, Y., Zhu, J. et al. Genetic analysis and gene fine mapping of a rolling leaf mutant (rl 11(t) ) in rice (Oryza sativa L.). Chin. Sci. Bull. 55, 1763–1769 (2010). https://doi.org/10.1007/s11434-010-3137-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11434-010-3137-0

Keywords

Search

Navigation