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Genome research profile of two Cordyceps sinensis cDNA libraries

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  • Bioinformatics
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Chinese Science Bulletin

Abstract

Cordyceps sinensis (CS) is well known as an ancient Chinese herb. It is used to expand bronchial smooth muscles, inhibit tumor growth and decrease blood pressure. Cordyceps sinensis is composed of two parts. One is the dead larvae body; the other is the stroma like withered grass. Up to now, few genome database articles about Cordyceps sinensis have been reported. In this study, two cDNA libraries were constructed using the worm part and grass part respectively for the first time. 12192 and 15456 clones from the worm-part (CSCA) and grass-part (CSBA) library were respectively picked. Sequences derived from CSCA were clustered into 1333 contigs and 2469 singlets, while those from CSBA were clustered into 1297 contigs and 2875 singlets. These ESTs include sequences representing a significant portion of proteins encoding genes in cell signalling, metabolism, information storage and processing. Some enzymes encoding genes were also found and linked with CS’s physiology such as proteases, peptidases, lipases and chitinase. Pairwise comparison between the two cDNA libraries was also studied. Some ESTs were found only in CSCA and some only in CSBA. Finally, a comparative genomics research was performed with Fusarium graminearum, Aspergillus nidulans, Neurospora crassa and Saccharomyces bayanus. The results indicate that the fungus’ genes maybe have complicated variation at the nucleic acid level, but the proteins translated are still conservative.

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Author information

Authors and Affiliations

  1. Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 100029, China

    ShaoPeng Zhang & XiaoYan Li

  2. Graduate University of Chinese Academy of Sciences, Beijing, 100049, China

    ShaoPeng Zhang & XiaoYan Li

  3. Staff Room of Traditional Chinese Medicine Diagnostics, Beijing University of Chinese Medicine, Beijing, 100029, China

    Hui Feng & YongSan Jin

  4. Hangzhou Genomics Institute, Hangzhou, 310008, China

    Wei Dong

  5. Beijing Genomics Institute, Beijing, 100029, China

    Wei Dong

Authors
  1. ShaoPeng Zhang
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  2. Hui Feng
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  3. XiaoYan Li
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  4. YongSan Jin
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  5. Wei Dong
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Correspondence to Wei Dong.

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These authors contributed equally to this work

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Zhang, S., Feng, H., Li, X. et al. Genome research profile of two Cordyceps sinensis cDNA libraries. Chin. Sci. Bull. 55, 1403–1411 (2010). https://doi.org/10.1007/s11434-010-0113-7

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  • DOI: https://doi.org/10.1007/s11434-010-0113-7

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