Abstract
The purpose of this study was to investigate the role of a human lens microRNA (miR-497-5p) in regulating epithelialmesenchymal transition (EMT) under the control of transforming growth factor beta (TGF-β). A microRNA array was used to evaluate the microRNA profiles of untreated and TGF-β-treated human lens epithelial cells in culture. This showed that TGF-β treatment led to the upregulation of 96 microRNAs and downregulation of 39 microRNAs. Thirteen microRNAs were predicted to be involved in the pathogenesis of posterior capsule opacification (PCO). Meanwhile, overexpression of miR-497-5p suppressed cell proliferation and EMT 48 h post-transfection, and inhibition of miR-497-5p accelerated cell proliferation and EMT. Treatment with TGF-β inhibited the expression of miR-497-5p, but not cell proliferation. miR-497-5p was also found to regulate the level of CCNE1 and FGF7, which are reported to be actively involved in EMT. CCNE1 and FGF7 were bona fide targets of miR-497-5p. The results suggest that miR-497-5p participates in the direct regulation of lens epithelial cell EMT and is regulated by TGF-β. miR-497-5p may be a novel target for PCO therapy.
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Acknowledgements
This work was supported by the Beijing New Star in Science and Technology (H020821380190 and Z131102000413025), the National Working Committee on Children and Women under State Council (2014108) and the National Natural Science Foundation of China (30471861).
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Wang, J., Zhang, J., Xiong, Y. et al. TGF-β regulation of microRNA miR-497-5p and ocular lens epithelial cell mesenchymal transition. Sci. China Life Sci. 63, 1928–1937 (2020). https://doi.org/10.1007/s11427-019-1603-y
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DOI: https://doi.org/10.1007/s11427-019-1603-y