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TiO2-functionalized mesoporous materials for sensitive analysis of multi-phosphopeptides

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Abstract

Protein phosphorylation as one of the most important post-translational modifications in mammalian cells regulates numerous biological processes. Here we propose a novel strategy for the selective isolation and sensitive analysis of multi-phosphopeptides based on TiO2-gratfed mesoporous materials, in which MCM-41 and SBA-15 were chosen as the hard templates. The commercialized IMAC and TiO2 nanopartices were further investigated in the phosphopeptide analysis for comparison. The enrichment efficiency was evaluated and measured by MALDI-TOF mass spectrometry. The results indicated that both TiO2-SBA-15 and TiO2-MCM-41 exhibited the preferential affinity to multi-phosphopeptides compared with the other two widely used strategies. The mesoporous TiO2 based protocol showed highly selective and sensitive properties, where phosphopeptides could be identified at femtomole.

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Authors and Affiliations

  1. Department of Chemistry and Institute of Biomedical Sciences, Fudan University, Shanghai, 200433, China

    WeiChao Guo, JingJing Wan, Kun Qian, ChengZhong Yu, JiLie Kong, PengYuan Yang & BaoHong Liu

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  1. WeiChao Guo
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  2. JingJing Wan
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  3. Kun Qian
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  4. ChengZhong Yu
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  5. JiLie Kong
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  7. BaoHong Liu
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Correspondence to BaoHong Liu.

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Guo, W., Wan, J., Qian, K. et al. TiO2-functionalized mesoporous materials for sensitive analysis of multi-phosphopeptides. Sci. China Chem. 54, 1327–1333 (2011). https://doi.org/10.1007/s11426-011-4344-5

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  • DOI: https://doi.org/10.1007/s11426-011-4344-5

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