Abstract
Bienzymatic biosensor for the determination of glucose by flow injection chemiluminescence (CL) detection was proposed. Hybrids of gold nanoparticles (GNPs) and chitosan were chosen as the immobilization matrix of glucose oxidase (GOD) and horseradish peroxidase (HRP) to fabricate the biosensors with silane-pretreated glass microbeads. After the enzyme catalyzing oxidation of glucose in GOD biosensor, the produced H2O2 flowed into HRP biosensor to react with luminol. The doped GNPs in chitosan were found to enhance the classical CL reaction of luminol-H2O2-HRP. The CL enhancement was investigated in detail by CL and UV-visible spectrum. Under the optimized experimental conditions, glucose could be determined in a linear range from 0.01 to 6.0 mmol/L with a detection limit of 5.0 μmol/L at 3σ. The accuracy of the proposed method was examined by detecting the glucose level in four clinical serum samples from hospital. The proposed method provides a new alternative to determine glucose.
Similar content being viewed by others
References
De Benedetto G E, Palmisano F, Zambonin P G. One-step fabrication of a bienzyme glucose sensor based on glucose oxidase and peroxidase immobilized onto a poly(pyrrole) modified glassy carbon electrode. Biosens Bioelectron, 1996, 11: 1001–1008
Tang F Q, Meng X W, Chen D, Ran J G, Zheng C Q. Glucose biosensor enhanced by nanoparticles. Sci China Ser B-Chem, 2000, 43: 268–274
Delvaux M, Walcarius A, Demoustier C S. Bienzyme HRP-GOx-modified gold nanoelectrodes for the sensitive amperometric detection of glucose at low overpotentials. Biosens Bioelectron, 2005, 20:1587–1594
Zhu L D, Yang R L, Zhai J L, Tian C Y. Bienzymatic glucose biosensor based on co-immobilization of peroxidase and glucose oxidase on a carbon nanotubes electrode. Biosens Bioelectron, 2007, 23: 528–535
Cai C X, Chen J, Lu T H. Direct electron transfer of glucose oxidase on the carbon nanotube electrode. Sci China Ser B-Chem, 2004, 47: 113–119
Ferri T, Maida S, Poscia A, Santucci R. A glucose biosensor based on electro-enzyme catalyzed oxidation of glucose using a HRP-GOD layered assembly. Electroanalysis, 2001,13: 1198–1202
Marquette C A, Blum L J. Luminol electrochemiluminescence-based fiber optical biosensors for flow injection analysis of glucose and lactate in natural samples. Anal Chim Acta, 1999, 381: 1–10
Wu Z S, Zhou G Z, Jiang J H, Shen G L, Yu R Q. Gold colloid-bienzyme conjugates for glucose detection utilizing surface-enhanced Raman scattering. Talanta, 2006, 70: 533–539
Zhu M, Huang X M, Shen H X. Microbial enzymatic assay of glucose in serum. Anal Chim Acta, 1997, 349: 165–170
Panoutsou P, Economou A. Rapid enzymatic chemiluminescent assay of glucose by means of a hybrid flow-injection/sequential-injection method. Talanta, 2005, 67: 603–609
Manera M, Miro M, Estela J M, Cerda V. A multisyringe flow injection system with immobilized glucose oxidase based on homogeneous chemiluminescence detection. Anal Chim Acta, 2004, 508: 23–30
Nicolau P, Manuel M, Jose M E, Victor C. Automated enzymatic assays in a renewable fashion using the multisyringe flow injection scheme with soluble enzymes. Anal Chem, 2004, 76: 773–780
Li B, Zhang Z, Jin Y. Plant tissue-based chemiluminescence flow biosensor for glycolic acid. Anal Chem, 2001, 73: 1203–1206
Zhang X R, Baeyenes W R G, Garcia C A M, Ouyang J. Recent developments in chemiluminescence sensors. Trends Anal Chem, 1999, 18: 384–391
Li B X, Lan D, Zhang Z J. Chemiluminescence flow-through biosensor for glucose with eggshell membrane as enzyme immobilization platform. Anal Biochem, 2008, 374: 64–70
Li B, Zhang Z. Chemiluminescence flow biosensor for determination of total D-amino acid in serum with immobilized reagents. Sensor Actuat B, 2000, 69: 70–74
Aboul-Enein H Y, Stefan R I, van Staden J F, Zhang X R, Garcia C A M, Baeyens W R G. FA non-potentiometric sensing method for the determination of sulfite. Crit Review Anal Chem, 2000, 30: 271–289
Kiba N, Miwa T, Tachibana M, Tani K, Koizumi H. Chemiluminometric sensor for simultaneous determination of L-glutamate and L-lysine with immobilized oxidases in a flow injection system. Anal Chem, 2002, 74: 1269–1274
Kricka L J, Voyta J C, Bronstein I. Chemiluminescent methods for detecting and quantitating enzyme activity. Methods Enzymol, 2000, 305: 370–390
Niazov T, Pavlov V, Xiao Y, Gill R, Willner I. DNAzyme-functionalized Au nanoparticles for the amplified detection of DNA or telomerase activity. Nano Lett, 2004, 4: 1683–1687
Wang Z P, Hu J Q, Jin Y, Yao X, Li J H. In situ amplified chemiluminescent detection of DNA and immunoassay of IgG using special-shaped gold nanoparticles as label. Clin Chem, 2006, 10: 1958–1961
Corgier B P, Li F, Blum L J, Marquette C A. On-chip chemiluminescent signal enhancement using nanostructured gold-modified carbon microarrays. Langmuir, 2007, 23(16): 8619–8623
Li Y X, Yang P, Wang P, Wang L. Development of a novel luminol chemiluminescent method catalyzed by gold nanoparticles for determination of estrogens. Anal Bioanal Chem, 2007, 387(2): 585–592
Wang L, Yang P, Li Y X, Chen H Q, Li M G, Luo F B. A flow injection chemiluminescence method for the determination of fluoroquinolone derivative using the reaction of luminol and hydrogen peroxide catalyzed by gold nanoparticles. Talanta, 2007, 72(3):1066–1072
Zhang Z F, Cui H, Lai C Z, Liu L J. Gold nanoparticle-catalyzed Luminol chemiluminescence and its analytical applications. Anal Chem, 2005, 77: 3324–3329
Lin J H, Yan F, Ju H X. Noncompetitive enzyme immunoassay for carcinoembryonic antigen by flow injection chemiluminescence. Clin Chim Acta, 2004, 341: 109–115
Lin J H, Yan F, Hu X Y, Ju H X. Chemiluminescent immunosensor for CA19-9 based on antigen immobilization on cross-linked chitosan membrane. J Immunol Methods, 2004, 291: 165–174
Hao C, Ding L, Zhang X J, Ju H X. Biocompatible conductive architecture of carbon nanofiber-doped chitosan prepared with controllable electrodeposition for cytosensing. Anal Chem, 2007, 79: 4442–4447
Huang H Z, Yang X R. Chitosan mediated assembly of gold nanoparticles multiplayer. Colloids Surf A: Physicochem Eng Aspects, 2003, 226: 77–86
Lin J H, Qu W, Zhang S S. Disposable biosensor based on enzyme immobilized on Au-chitosan modified ITO electrode with flow injection amperometric analysis. Anal Biochem, 2007, 307: 288–293
Wallace W T, Whetten R L. Coadsorption of CO and O2 on selected gold clusters: Evidence for efficient room-temperature CO2 generation. J Am Chem Soc, 2002, 124: 7499–7505
Dodeigne C, Thunus L, Lejeune R. Chemiluminescence as diagnostic tool. Talanta, 2000, 51: 415–439
Author information
Authors and Affiliations
Corresponding author
Additional information
Supported by the Natural Science Foundation of Shandong Province (Grant No. Q2007B03), the Doctoral Fund of Qingdao University of Science and Technology (Grant No. 0022141), and the National Natural Science Foundation of China (Grant No. 20775038)
Rights and permissions
About this article
Cite this article
Lin, J., Zhang, H. & Zhang, S. New bienzymatic strategy for glucose determination by immobilized-gold nanoparticle-enhanced chemiluminescence. Sci. China Ser. B-Chem. 52, 196–202 (2009). https://doi.org/10.1007/s11426-008-0152-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11426-008-0152-y