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Ceramicine B, a limonoid with anti-lipid droplets accumulation activity from Chisocheton ceramicus

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Abstract

The Meliaceae family of plants has been shown to contain a vast number of compounds with the potential to be developed for medicinal purposes. We have previously reported the isolation of limonoids from a plant in the Meliaceae family named Chisocheton ceramicus. Ceramicine B was identified as an active compound in inhibiting lipid droplets accumulation (LDA) in the mouse preadipocyte cell line MC3T3-G2/PA6. The presence of ceramicine B was found to inhibit the expression of glucose transporter type 4, lipoprotein lipase, and 11-beta hydroxysteroid dehydrogenase mRNA, and also adipogenic master regulator, peroxisome proliferator-activated receptor-γ, and CCAAT-enhancer-binding protein-α (C/EBPα) mRNA. However, for early adipogenic regulators, such as C/EBPβ and C/EBPδ, and intermediary adipogenic regulators, Krüppel-like factors were unaffected. Western blot analysis showed that ceramicine B was found to inhibit the phosphorylation of Forkhead box O1 (Foxo1), a key process in the insulin signaling pathway. This suggested that the mechanism of anti-LDA activity of ceramicine B was partly via the inhibition of Foxo1 phosphorylation.

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Acknowledgments

This work was partly supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and a Grant from the Open Research Center Project.

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Hoshi University, Ebara 2-4-41, Shinagawa-ku, Tokyo, 142-8501, Japan

    Chin Piow Wong, Toshio Kaneda & Hiroshi Morita

  2. Department of Chemistry, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia

    A. Hamid A. Hadi

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  1. Chin Piow Wong
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  4. Hiroshi Morita
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Correspondence to Hiroshi Morita.

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Wong, C.P., Kaneda, T., Hadi, A.H.A. et al. Ceramicine B, a limonoid with anti-lipid droplets accumulation activity from Chisocheton ceramicus . J Nat Med 68, 22–30 (2014). https://doi.org/10.1007/s11418-013-0755-2

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  • DOI: https://doi.org/10.1007/s11418-013-0755-2

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