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Altered left ventricular performance in aging physically active mice with an ankle sprain injury

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We assessed the impact of differing physical activity levels throughout the lifespan, using a musculoskeletal injury model, on the age-related changes in left ventricular (LV) parameters in active mice. Forty male mice (CBA/J) were randomly placed into one of three running wheel groups (transected CFL group, transected ATFL/CFL group, SHAM group) or a SHAM Sedentary group (SHAMSED). Before surgery and every 6 weeks after surgery, LV parameters were measured under 2.5 % isoflurane inhalation. Group effects for daily distance run was significantly greater for the SHAM and lesser for the ATLF/CFL mice (p = 0.013) with distance run decreasing with age for all mice (p < 0.0001). Beginning at 6 months of age, interaction (group × age) was noted with LV posterior wall thickness-to-radius ratios (h/r) where h/r increased with age in the ATFL/CFL and SHAMSED mice while the SHAM and CFL mice exhibited decreased h/r with age (p = 0.0002). Passive filling velocity (E wave) was significantly greater in the SHAM mice and lowest for the ATFL/CFL and SHAMSED mice (p < 0.0001) beginning at 9 months of age. Active filling velocity (A wave) was not different between groups (p = 0.10). Passive-to-active filling velocity ratio (E/A ratio) was different between groups (p < 0.0001), with higher ratios for the SHAM mice and lower ratios for the ATFL/CFL and SHAMSED mice in response to physical activity beginning at 9 months of age. Passive-to-active filling velocity ratio decreased with age (p < 0.0001). Regular physical activity throughout the lifespan improved LV structure, passive filling velocity, and E/A ratio by 6 to 9 months of age and attenuated any negative alterations throughout the second half of life. The diastolic filling differences were found to be significantly related to the amount of activity performed by 9 months and at the end of the lifespan.

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Acknowledgments

The work was supported by grants from the University of North Carolina at Charlotte Faculty Research Grants Program. The authors would like to thank Nastassia Brown, Jason Cline, Karen Correa, Kristen Regan, and Robert Valderama for their assistance with animal care and data collection.

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Authors and Affiliations

  1. Laboratory of Systems Physiology, Department of Kinesiology, University of North Carolina at Charlotte, Charlotte, NC, USA

    Michael J. Turner, Sophie Guderian, Joshua R. Huot, Bailey D. Peck, Susan T. Arthur & Joseph S. Marino

  2. Biodynamics Research Laboratory, Department of Kinesiology, University of North Carolina at Charlotte, Charlotte, NC, USA

    Erik A. Wikstrom & Tricia Hubbard-Turner

  3. Center for Biomedical Engineering & Science, University of North Carolina at Charlotte, Charlotte, NC, USA

    Erik A. Wikstrom & Tricia Hubbard-Turner

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  1. Michael J. Turner
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  2. Sophie Guderian
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  3. Erik A. Wikstrom
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  4. Joshua R. Huot
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  5. Bailey D. Peck
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  6. Susan T. Arthur
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  8. Tricia Hubbard-Turner
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Correspondence to Michael J. Turner.

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This study was funded by the UNC Charlotte Faculty Research Grants Program.

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The authors declare that they have no competing interests.

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Turner, M.J., Guderian, S., Wikstrom, E.A. et al. Altered left ventricular performance in aging physically active mice with an ankle sprain injury. AGE 38, 15 (2016). https://doi.org/10.1007/s11357-016-9877-2

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