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Oxalic acid-assisted phytoextraction of heavy metal contaminated wastewater through Lemna minor L.

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Abstract

The present research was carried out to investigate the performance of oxalic acid (OA) through Lemna minor L. (duckweed) in the phytoextraction of lead (Pb) from metal contaminated water. Zero, 100 μM, 250 μM, and 500 μM Pb concentration and combinations with 2.5 ml of OA were provided to the plants in the form of solution after defining intervals. Continuous aeration was provided to the plants and kept a pH level at 6.5. Results from this research depicted that increasing concentration level of Pb inhibited the overall plant growth, biomass, frond area, chlorophyll, and antioxidant enzyme activities like peroxidase (POD), superoxide-dismutase (SOD), catalases (CAT), and ascorbate-peroxidase (APX). Moreover, Pb stress enhances the concentration, hydrogen peroxide, malondialdehyde, and electrolyte leakage substances in plants. Furthermore, the addition of OA alleviated the Pb-induced toxicity in the plants, increasing the Pb accumulation and its endorsement in the L. minor. The OA addition increased the Pb accumulation in plants at 0, 100, 250 and 500 μM. At higher concentration, Pb showed harmful effect as related to the other low doses. Under the application of OA, Pb higher accumulation and concentration in L. minor were measured, which showed that it could be the most suitable plant for the phytoextraction of lead-contaminated soil and wastewater.

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This study is a master of philosophy lab scale research work, and the journal has full rights to publish this research. The data will be provided on the demand of the journal.

References

  • Aebi H (1984) Catalase in vitro. In: Methods in enzymology, vol 105. AP, pp 121–126

  • Afshan S, Ali S, Bharwana SA, Rizwan M, Farid M, Abbas F, Abbasi GH (2015) Citric acid enhances the phytoextraction of chromium, plant growth, and photosynthesis by alleviating the oxidative damages in Brassica napus L. ESPR. 22:11679–11689

    CAS  Google Scholar 

  • Aken BV, Doty SL (2009) Transgenic plants and associated bacteria for phytoremediation of chlorinated compounds. Biotechnol Genet Eng Rev 26(1):43–64

  • Ali B, Wang B, Ali S, Ghani MA, Hayat MT, Yang C, Zhou WJ (2013) 5-Aminolevulinic acid ameliorates the growth, photosynthetic gas exchange capacity, and ultrastructural changes under cadmium stress in Brassica napus L. J Plant Growth Regul 32(3):604–614

    CAS  Google Scholar 

  • Ali S, Zeng F, Cai S, Qiu B, Zhang G (2011) The interaction of salinity and chromium in the influence of barley growth and oxidative stress. Plant Soil Environ 57:153–159

  • Appenroth KJ, Krech K, Keresztes A, Fischer W, Koloczek H (2010) Effects of nickel on the chloroplasts of the duckweeds Spirodela polyrhiza and Lemna minor L. and their possible use in biomonitoring and phytoremediation. Chemosphere. 78(3):216–223

    CAS  Google Scholar 

  • Axtell NR, Sternberg SP, Claussen K (2003) Lead and nickel removal using Microspora and Lemna minor. Bioresour Technol 89(1):41–48

    CAS  Google Scholar 

  • Balen EV, van de Geijn SC, Desmet GM (1980) Autoradiographic evidence for the incorporation of cadmium into calcium oxalate crystals (heavy metal)[tomatoes]. Zeitschrift fuer Pflanzenphysiologie (Germany, FR)

  • Bhargava A, Carmona FF, Bhargava M, Srivastava S (2012) Approaches for enhanced phytoextraction of heavy metals. J Environ Manag 105:103–120

    CAS  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72(1-2):248–254

    CAS  Google Scholar 

  • Brooks RR (1998) Plants that hyperaccumulate heavy metals, their role in phytoremediation, microbiology, archaeology, mineral exploration and phytomining

  • Cunningham SD, Ow DW (1996) Promises and prospects of phytoremediation. Plant Physiol 110(3):715

    CAS  Google Scholar 

  • Dembitsky VM, Rezanka T (2003) Natural occurrence of arseno compounds in plants, lichens, fungi, algal species, and microorganisms. Plant Sci 165(6):1177–1192

    CAS  Google Scholar 

  • Dhindsa RS, Plumb-Dhindsa P, Thorpe TA (1981) Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. JXB 32(1):93–101

    CAS  Google Scholar 

  • Dionisio-Sese ML, Tobita S (1998) Antioxidant responses of rice seedlings to salinity stress. Plant Sci 135(1):1–9

    CAS  Google Scholar 

  • Diwan H, Ahmad A, Iqbal M (2012) Chromium-induced alterations in photosynthesis and associated attributes in Indian mustard. J Environ Biol 33(2):239

    CAS  Google Scholar 

  • Farid M, Shakoor MB, Ehsan S, Ali S, Zubair M, Hanif MS (2013) Morphological, physiological and biochemical responses of different plant species to Cd stress. Int J Chem Biochem Sci 3:53–60

    Google Scholar 

  • Ghani A (2011) Effect of chromium toxicity on growth, chlorophyll and some mineral nutrients of Brassica juncea L. Egypt. Acad J Biol Sci 2(1):9–15

    Google Scholar 

  • Ghosh M, Singh SP (2005) A review on phytoremediation of heavy metals and utilization of it’s by products. Asian J Energy Environ 6(4):18

    Google Scholar 

  • Gill RA, Zang L, Ali B, Farooq MA, Cui P, Yang S, Zhou W (2015) Chromium-induced physio-chemical and ultrastructural changes in four cultivars of Brassica napus L. Chemosphere 120:154–164

    CAS  Google Scholar 

  • Gratão PL, Polle A, Lea PJ, Azevedo RA (2005) Making the life of heavy metal-stressed plants a little easier. Funct Plant Biol 32(6):481–494

    Google Scholar 

  • Habiba U, Ali S, Farid M, Shakoor MB, Rizwan M, Ibrahim M, Ali B (2015) EDTA enhanced plant growth, antioxidant defense system, and phytoextraction of copper by Brassica napus L. Environ Sci Pollut Res 22(2):1534–1544

    CAS  Google Scholar 

  • Haouari CC, Nasraoui AH, Bouthour D, Houda MD, Daieb CB, Mnai J, Gouia H (2012) Response of tomato (Solanum lycopersicon) to cadmium toxicity: growth, element uptake, chlorophyll content and photosynthesis rate. African J Plant Sci 6(1):001–007

    CAS  Google Scholar 

  • Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125(1):189–198

    CAS  Google Scholar 

  • Hou W, Chen X, Song G, Wang Q, Chang CC (2007) Effects of copper and cadmium on heavy metal polluted waterbody restoration by duckweed (Lemna minor). Plant Physiol Biochem 45(1):62–69

    CAS  Google Scholar 

  • Ikhuoria EU, Okieimen FE (2000) Scavenging cadmium, copper, lead, nickel and zinc ions from aqueous solution by modified cellulosic sorbent. Int J Environ Sci 57(4):401–409

    CAS  Google Scholar 

  • Jabeen N, Abbas Z, Iqbal M, Rizwan M, Jabbar A, Farid M, Abbas F (2016) Glycinebetaine mediates chromium tolerance in mung bean through lowering of Cr uptake and improved antioxidant system. Arch Agron Soil Sci 62(5):648–662

    CAS  Google Scholar 

  • Júnior CA, de Sousa BH, Galazzi RM, Koolen HH, Gozzo FC, Arruda MA (2015) Evaluation of proteome alterations induced by cadmium stress in sunflower (Helianthus annuus L.) cultures. Ecotoxicol Environ Saf 119:170–177

    Google Scholar 

  • Kang JW (2014) Removing environmental organic pollutants with bioremediation and phytoremediation. Biotechnol Lett 36(6):1129–1139

    CAS  Google Scholar 

  • Keller C, Rizwan M, Davidian JC, Pokrovsky OS, Bovet N, Chaurand P, Meunier JD (2015) Effect of silicon on wheat seedlings (Triticum turgidum L.) grown in hydroponics and exposed to 0 to 30 μM Cu. Planta 241(4):847–860

    CAS  Google Scholar 

  • Khlifi R, Hamza-Chaffai A (2010) Head and neck cancer due to heavy metal exposure via tobacco smoking and professional exposure: a review. Toxicol Appl Pharmacol 248(2):71–88

    CAS  Google Scholar 

  • Manohar S, Jadia C, Fulekar MH (2007) Impact of ganesh idol immersion on water quality. Indian J Environ Prot 27(3):216

    CAS  Google Scholar 

  • Metzner H, Rau H, Senger H (1965) Untersuchungenzursynchronisierbaketieinzel- nerpigmentmangel-mutation von chlorella. Planta 65:186–194

    CAS  Google Scholar 

  • Mishra VK, Tripathi BD (2008) Concurrent removal and accumulation of heavy metals by the three aquatic macrophytes. Bioresour Technol 99(15):7091–7097

    CAS  Google Scholar 

  • Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7(9):405–410

    CAS  Google Scholar 

  • Mittler R, Vanderauwera S, Gollery M, Van Breusegem F (2004) Reactive oxygen gene network of plants. Trends Plant Sci 9:490–498

  • Monalisa M, Kumar PH (2013) Effect of ionic and chelate assisted hexavalent chromium on mung bean seedlings (Vigna radiata L. wilczek. var k-851) during seedling growth. J. stress physiol. biochem. 9(2)

  • Nagajyoti PC, Lee KD, Sreekanth TVM (2010) Heavy metals, occurrence and toxicity for plants: a review. Environ Chem Lett 8(3):199–216

    CAS  Google Scholar 

  • Najeeb U, Ahmad W, Zia MH, Zaffar M, ZhouW. (2017) Enhancing the lead phytostabilization in wetland plant Juncus effusus L. through somaclonal manipulation and EDTA enrichment. Arab J Chem 10:S3310–S3317

    CAS  Google Scholar 

  • Najeeb U, Jilani G, Ali S, Sarwar M, Xu L, Zhou W (2011) Insights into cadmium induced physiological and ultra-structural disorders in Juncus effusus L. and its remediation through exogenous citric acid. J Hazard Mater 186(1):565–574

    CAS  Google Scholar 

  • Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22(5):867–880

    CAS  Google Scholar 

  • Pandey VC, Singh JS, Singh RP, Singh N, Yunus M (2011) Arsenic hazards in coal fly ash and its fate in Indian scenario. Resour Conserv Recycl 55(9-10):819–835

    Google Scholar 

  • Parmar P, Kumari N, Sharma V (2013) Structural and functional alterations in photosynthetic apparatus of plants under cadmium stress. Bot Stud 54:45

    Google Scholar 

  • Prasad MNV (2003) Phytoremediation of metal-polluted ecosystems: hype for commercialization. Russ J Plant Physiol 50(5):686–701

    CAS  Google Scholar 

  • Prasad R, Shivay YS (2017) Oxalic acid/oxylates in plants: from self-defence to phytoremediation. Curr Sci 112:1665–1667

    CAS  Google Scholar 

  • Rodríguez MC, Barsanti L, Passarelli V, Evangelista V et al (2007) Effects of chromium on photosynthetic and photoreceptive apparatus of the alga Chlamydomonas reinhardtii. Environ Res 105(2):234–239

    Google Scholar 

  • Sallah-Ud-Din R, Farid M, Saeed R, Ali S, Rizwan M, Tauqeer HM, Bukhari SAH (2017) Citric acid enhanced the antioxidant defense system and chromium uptake by Lemna minor L. grown in hydroponics under Cr stress. ESPR. 24:17669–17678

    CAS  Google Scholar 

  • Shanker AK, Cervantes C, Loza-Tavera H, Avudainayagam S (2005) Chromium toxicity in plants. Environ Int 31(5):739–753

    CAS  Google Scholar 

  • Singh D, Tiwari A, Gupta R (2012) Phytoremediation of lead from wastewater using aquatic plants. J Adv Agric Technol 8(1):1–11

    Google Scholar 

  • Sood A, Uniyal PL, Prasanna R, Ahluwalia AS (2012) Phytoremediation potential of aquatic macrophyte, Azolla. Ambio 41:122–137

    CAS  Google Scholar 

  • Wang W (1990) Literature review on duckweed toxicity testing. Environ Res 52(1):7–22

    CAS  Google Scholar 

  • Wolińska A, Stępniewska Z, Włosek R (2013) The influence of old leather tannery district on chromium contamination of soils, water and plants. J Nat Sci 5(02):253

    Google Scholar 

  • Ye WL, Khan MA, McGrath SP, Zhao FJ (2011) Phytoremediation of arsenic contaminated paddy soils with Pteris vittata markedly reduces arsenic uptake by rice. Environ Pollut 159(12):3739–3743

    CAS  Google Scholar 

  • Zaheer IE, Ali S, Rizwan M, Farid M, Shakoor MB, Gill RA, Najeeb U, Iqbal N, Ahmad R (2015) Citric acid assisted phytoremediation of copper by Brassica napus L. Ecotoxicol Environ Saf 120:310–317

    CAS  Google Scholar 

  • Zayed A, Gowthaman S, Terry N (1998) Phytoaccumulation of trace elements by wetland plants: I. Duckweed. J Environ Qual 27(3):715–721

    CAS  Google Scholar 

  • Zhang J, Kirkham MB (1994) Drought-stress-induced changes in activities of superoxide dismutase, catalase, and peroxidase in wheat species. Plant Cell Physiol 35(5):785–791

    CAS  Google Scholar 

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  1. Faculty of Science, Department of Environmental Sciences, University of Gujrat (Hafiz Hayat Campus), Gujrat, Punjab, Pakistan

    Umer Hayat & Shafuq Abbas

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  1. Umer Hayat
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Umer Hayat and Shafuq Abbas carried out the experiment. Umer Hayat wrote the manuscript and supervised the project. Shafuq Abbas contributed to the analysis of the results. All authors contributed to the final manuscript.

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Correspondence to Umer Hayat.

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Hayat, U., Abbas, S. Oxalic acid-assisted phytoextraction of heavy metal contaminated wastewater through Lemna minor L.. Environ Sci Pollut Res 30, 103972–103982 (2023). https://doi.org/10.1007/s11356-023-29547-w

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