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Bioreduction and mineralization of Cr(VI) by Sporosarcina saromensis W5 induced carbonate precipitation

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Abstract

The microbial reduction of Cr(VI) to Cr(III) is widely applied, but most studies ignored the stability of reduction products. In this study, the Cr(VI)-reducing bacterium of Sporosarcina saromensis combined with microbially induced carbonate precipitation (MICP) was used to explore the reduction and mineralization mechanisms of Cr(VI). The results indicated that the high concentration of Ca2+ could significantly enhance the reduction and mineralization of Cr(VI). The highest reduction and mineralization efficiencies of 99.5% and 55.9% were achieved at 4 g/L Ca2+. Moreover, the urease activity of S. saromensis in the experimental group was up to 13.28 U/mg NH3-N. Besides, the characteristic results revealed that Cr(VI) and reduced Cr(III) were absorbed on the surface or got into the interspace of CaCO3, which produced a new stable phase (Ca10Cr6O24(CO3)). Overall, the combination of S. saromensis and MICP technology might be a high-efficiency and environmentally friendly strategy for further application in the Cr(VI)-containing groundwater.

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References

  • Ali A, Li M, Su J, Li Y, Wang Z, Bai Y, Ali EF, Shaheen SM (2022) Brevundimonas diminuta isolated from mines polluted soil immobilized cadmium (Cd2+) and zinc (Zn2+) through calcium carbonate precipitation: microscopic and spectroscopic investigations. Sci Total Environ 813:152668

  • Azeez NA, Dash SS, Gummadi SN, Deepa VS (2021) Nano-remediation of toxic heavy metal contamination: hexavalent chromium Cr(VI). Chemosphere 266:129204

  • Bai H, Liu D, Zheng W, Ma L, Yang S, Cao J, Lu X, Wang H, Mehta N (2021) Microbially-induced calcium carbonate precipitation by a halophilic ureolytic bacterium and its potential for remediation of heavy metal-contaminated saline environments. Int Biodeterior Biodegrad 165:105311

  • Cao X, Xu L, Chen YP, Decho AW, Cui Z, Lead JR (2022) Contribution, composition, and structure of EPS by in vivo exposure to elucidate the mechanisms of nanoparticle-enhanced bioremediation to metals. Environ Sci Technol 56:896–906

    Article  CAS  Google Scholar 

  • Chen C (2021) Quality control in water quality determination of ammonia nitrogen-salicylic acid spectrophotometry. In: Environmental Engineering Technology Innovation and Application Sub-Conference of 2021 Annual Science and Technology Conference of Chinese Society for Environmental Sciences, Tianjin, China, pp 106–109

  • Das S, Mishra J, Das SK, Pandey S, Rao DS, Chakraborty A, Sudarshan M, Das N, Thatoi H (2014) Investigation on mechanism of Cr(VI) reduction and removal by Bacillus amyloliquefaciens, a novel chromate tolerant bacterium isolated from chromite mine soil. Chemosphere 96:112–121

    Article  CAS  Google Scholar 

  • Fang L, Niu Q, Cheng L, Jiang J, Yu Y-Y, Chu J, Achal V, You T (2021) Ca-mediated alleviation of Cd2+ induced toxicity and improved Cd2+ biomineralization by Sporosarcina pasteurii. Sci Total Environ 787:147627

  • Fang Z, Liu W, Yao T, Zhou G, Wei S, Qin L (2022) Experimental study of chromium (III) coprecipitation with calcium carbonate. Geochim Cosmochim Ac 322:94–108

    Article  CAS  Google Scholar 

  • Fu L, Feng A, Xiao J, Wu Q, Ye Q, Peng S (2021) Remediation of soil contaminated with high levels of hexavalent chromium by combined chemical-microbial reduction and stabilization. J Hazard Mater 403:123847

  • He N, Hu L, He Z, Li M, Huang Y (2022a) Mineralization of lead by Phanerochaete chrysosporium microcapsules loaded with hydroxyapatite. J Hazard Mater 422:126902

  • He N, Hu L, Jiang C, Li M (2022b) Remediation of chromium, zinc, arsenic, lead and antimony contaminated acidic mine soil based on Phanerochaete chrysosporium induced phosphate precipitation. Sci Total Environ 850:157995

    Article  CAS  Google Scholar 

  • Huang X-N, Min D, Liu D-F, Cheng L, Qian C, Li W-W, Yu H-Q (2019) Formation mechanism of organo-chromium (III) complexes from bioreduction of chromium (VI) by Aeromonas hydrophila. Environ Int 129:86–94

    Article  CAS  Google Scholar 

  • Huang Y, Zeng Q, Hu L, Xiong D, Zhong H, He Z (2021a) Column study of enhanced Cr(VI) removal and removal mechanisms by Sporosarcina saromensis W5 assisted bio-permeable reactive barrier. J Hazard Mater 405:124115

    Article  CAS  Google Scholar 

  • Huang Y, Zeng Q, Hu L, Zhong H, He Z (2021b) Bioreduction performances and mechanisms of Cr(VI) by Sporosarcina saromensis W5, a novel Cr(VI)-reducing facultative anaerobic bacteria. J Hazard Mater 413:125411

  • Jiang X, Long W, Peng L, Xu T, He F, Tang Y, Zhang W (2022) Reductive immobilization of Cr(VI) in contaminated water by tannic acid. Chemosphere 297:134081

  • Karthik C, Barathi S, Pugazhendhi A, Ramkumar VS, Thi NBD, Arulselvi PI (2017) Evaluation of Cr(VI) reduction mechanism and removal by Cellulosimicrobium funkei strain AR8, a novel haloalkaliphilic bacterium. J Hazard Mater 333:42–53

    Article  CAS  Google Scholar 

  • Karthik C, Ramkumar VS, Pugazhendhi A, Gopalakrishnan K, Arulselvi PI (2017) Biosorption and biotransformation of Cr(VI) by novel Cellulosimicrobium funkei strain AR6. J Taiwan Inst Chem E 70:282–290

    Article  CAS  Google Scholar 

  • Li W, Chen W-S, Zhou P-P, Cao L, Yu L-J (2013) Influence of initial pH on the precipitation and crystal morphology of calcium carbonate induced by microbial carbonic anhydrase. Colloids Surf B 102:281–287

    Article  CAS  Google Scholar 

  • Li F, Zheng Y, Tian J, Ge F, Liu X, Tang Y, Feng C (2019) Cupriavidus sp. strain Cd02-mediated pH increase favoring bioprecipitation of Cd2+ in medium and reduction of cadmium bioavailability in paddy soil. Ecotoxicol Environ Saf 184:109655

  • Li L, Xu Y, Zhong D, Zhong N (2020a) CTAB-surface-functionalized magnetic MOF@MOF composite adsorbent for Cr(VI) efficient removal from aqueous solution. Colloids Surf 586:124255

  • Li Y, Wang H, Wu P, Yu L, Rehman S, Wang J, Yang S, Zhu N (2020b) Bioreduction of hexavalent chromium on goethite in the presence of Pseudomonas aeruginosa. Environ Pollut 265:114765

  • Li L, Shang X, Sun X, Xiao X, Xue J, Gao Y, Gao H (2021) Bioremediation potential of hexavalent chromium by a novel bacterium Stenotrophomonas acidaminiphila 4–1. Environ Technol Innov 22:101409

    Article  CAS  Google Scholar 

  • Li M, Ali A, Li Y, Su J, Zhang S (2022) The performance and mechanism of simultaneous removal of calcium and heavy metals by Ochrobactrum sp. GMC12 with the chia seed (Salvia hispanica) gum as a synergist. Chemosphere 297:134061

    Article  CAS  Google Scholar 

  • Liu M, Cai L, Luo H (2022) Effect of nano-silica on microbiologically induced calcium carbonate precipitation. Constr Build Mater 314:125661

  • Luo Y, Ye B, Ye J, Pang J, Xu Q, Shi J, Long B, Shi J (2020) Ca2+ and SO42− accelerate the reduction of Cr(VI) by Penicillium oxalicum SL2. J Hazard Mater 382:121072

    Article  CAS  Google Scholar 

  • Luo X, Zhou X, Peng C, Shao P, Wei F, Li S, Liu T, Yang L, Ding L, Luo X (2022) Bioreduction performance of Cr(VI) by microbial extracellular polymeric substances (EPS) and the overlooked role of tryptophan. J Hazard Mater 433:128822

  • Munir MAM, Liu G, Yousaf B, Ali MU, Cheema AI, Rashid MS, Rehman A (2020) Bamboo-biochar and hydrothermally treated-coal mediated geochemical speciation, transformation and uptake of Cd, Cr, and Pb in a polymetal(iod)s-contaminated mine soil. Environ Pollut 265:114816

  • Naveed M, Duan J, Uddin S, Suleman M, Hui Y, Li H (2020) Application of microbially induced calcium carbonate precipitation with urea hydrolysis to improve the mechanical properties of soil. Ecol Eng 153:105885

  • Okwadha GDO, Li J (2010) Optimum conditions for microbial carbonate precipitation. Chemosphere 81:1143–1148

    Article  CAS  Google Scholar 

  • Pradhan D, Sukla LB, Sawyer M, Rahman PKSM (2017) Recent bioreduction of hexavalent chromium in wastewater treatment: A review. J Ind Eng Chem 55:1–20

    Article  CAS  Google Scholar 

  • Qian X, Fang C, Huang M, Achal V (2017) Characterization of fungal-mediated carbonate precipitation in the biomineralization of chromate and lead from an aqueous solution and soil. J Clean Prod 164:198–208

    Article  CAS  Google Scholar 

  • Qin J, Qin Q, Li X, Xue J, Wang R, Zhang Q, Wang P, Guo Z, Gong Y (2021) Urea supply control in microbial carbonate precipitation to effectively fill pores of concrete. Constr Build Mater 310:125123

  • Sarankumar RK, Arulprakash A, Devanesan S, Selvi A, AlSalhi MS, Rajasekar A, Ahamed A (2020) Bioreduction of hexavalent chromium by chromium resistant alkalophilic bacteria isolated from tannery effluent. J King Saud Univ Sci 32:1969–1977

    Article  Google Scholar 

  • Song M, Ju T, Meng Y, Han S, Lin L, Jiang J (2022) A review on the applications of microbially induced calcium carbonate precipitation in solid waste treatment and soil remediation. Chemosphere 290:133229

  • Tan H, Wang C, Zeng G, Luo Y, Li H, Xu H (2020) Bioreduction and biosorption of Cr(VI) by a novel Bacillus sp. CRB-B1 strain. J Hazard Mater 386:121628

  • Xiang J, Qiu J, Wang Y, Gu X (2022) Calcium acetate as calcium source used to biocement for improving performance and reducing ammonia emission. J Clean Prod 348:131286

  • Xie Q, Liu N, Lin D, Qu R, Zhou Q, Ge F (2020) The complexation with proteins in extracellular polymeric substances alleviates the toxicity of Cd (II) to Chlorella vulgaris. Environ Pollut 263:114102

  • Xu G, Li D, Jiao B, Li D, Yin Y, Lun L, Zhao Z, Li S (2017) Biomineralization of a calcifying ureolytic bacterium Microbacterium sp. GM-1. Electron J Biotechnol 25:21–27

    Article  CAS  Google Scholar 

  • Xue Z-F, Cheng W-C, Wang L, Hu W (2022) Effects of bacterial inoculation and calcium source on microbial-induced carbonate precipitation for lead remediation. J Hazard Mater 426:128090

    Article  CAS  Google Scholar 

  • Yi H, Zheng T, Jia Z, Su T, Wang C (2021) Study on the influencing factors and mechanism of calcium carbonate precipitation induced by urease bacteria. J Cryst Growth 564:126113

  • Zeng Y, Chen Z, Du Y, Lyu Q, Yang Z, Liu Y, Yan Z (2021) Microbiologically induced calcite precipitation technology for mineralizing lead and cadmium in landfill leachate. J Environ Manage 296:113199

  • Zhang J, Su P, Li Y, Li L (2021) Environmental investigation of bio-modification of steel slag through microbially induced carbonate precipitation. J Environ Sci 101:282–292

    Article  CAS  Google Scholar 

  • Zhang J, Su P, Li L (2022) Bioremediation of stainless steel pickling sludge through microbially induced carbonate precipitation. Chemosphere 298:134213

  • Zhao X, Wang M, Wang H, Tang D, Huang J, Sun Y (2019) Study on the remediation of Cd pollution by the biomineralization of urease-producing bacteria. Int J Environ Res Public Health 16:268

  • Zhao X, Tang D, Jiang Y (2021) Effect of the reduction-mineralization synergistic mechanism of Bacillus on the remediation of hexavalent chromium. Sci Total Environ 777:146190

  • Zheng T (2021) Bacteria-induced facile biotic calcium carbonate precipitation. J Cryst Growth 563:126096

    Article  CAS  Google Scholar 

  • Zheng T, Qian C (2020) Influencing factors and formation mechanism of CaCO3 precipitation induced by microbial carbonic anhydrase. Process Biochem 91:271–281

    Article  CAS  Google Scholar 

  • Zheng Y, Xiao C, Chi R (2021) Remediation of soil cadmium pollution by biomineralization using microbial-induced precipitation: a review. World J Microbiol Biotechnol 37:208

    Article  CAS  Google Scholar 

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Funding

This work was financially supported by the National Natural Science Foundation of China (52270172, 51909281) and the Key Research and Development International Cooperation Project (2022YFE0119600).

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Authors and Affiliations

  1. School of Minerals Processing and Bioengineering, Key Laboratory of Biohydrometallurgy of Ministry of Education, Central South University, Changsha, 410083, China

    Chunyangzi Jiang, Liang Hu, Ni He, Yayuan Liu & Hongbo Zhao

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  1. Chunyangzi Jiang
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  2. Liang Hu
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  3. Ni He
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  4. Yayuan Liu
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  5. Hongbo Zhao
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Contributions

Chunyangzi Jiang conducted the experiments, analyzed the data, and produced the data displays and original manuscript preparation. Ni He improved the manuscript. Yayuan Liu processed the data. Liang Hu conceived original ideas, supervision, funding acquisition, and reviewed and edited the manuscript. Hongbo Zhao contributed to funding acquisition. All the authors read and approved the manuscript.

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Correspondence to Liang Hu.

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Jiang, C., Hu, L., He, N. et al. Bioreduction and mineralization of Cr(VI) by Sporosarcina saromensis W5 induced carbonate precipitation. Environ Sci Pollut Res 30, 89355–89368 (2023). https://doi.org/10.1007/s11356-023-28536-3

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