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Transcriptomic analysis of lipid metabolism in zebrafish offspring of parental long-term exposure to bisphenol A

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Abstract

Bisphenol A (BPA) is one of the most common environmental endocrine disruptor chemicals (EDCs) and exhibits reproductive, cardiovascular, immune, and neurodevelopmental toxic effects. The development of the offspring was examined in the present investigation to determine the cross-generational effects of long-term exposure of parental zebrafish to environmental concentrations of BPA (15 and 225 µg/L). Parents were exposed to BPA for 120 days, and their offspring were evaluated at 7 days after fertilization in BPA-free water. The offspring exhibited higher mortality, deformity, and heart rates, and showed significant fat accumulation in abdominal region. RNA-Seq data showed that more lipid metabolism-related KEGG pathways, such as the PPAR signaling pathway, adipocytokine signaling pathway, and ether lipid metabolism pathway were enriched in the 225 µg/L BPA-treated offspring compared to 15 µg/L BPA-treated offspring, indicating greater effects of high dose BPA on offspring lipid metabolism. Lipid metabolism-related genes implied that BPA is responsible for disrupting lipid metabolic processes in the offspring through increased lipid production, abnormal transport, and disruption of lipid catabolism. The present study will be helpful for further evaluation of the reproductive toxicity of environmental BPA to organisms and the subsequent parent-mediated intergenerational toxicity.

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Data availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Funding

Financial support for this study was provided by Postgraduate Research & Practice Innovation Program of Jiangsu Province (KYCX22_3557), National Natural Science Foundation of China (31800435), Postdoctoral Research Foundation of China (2020M681743), and Postdoctoral Science Foundation of Jiangsu Province (2020Z104).

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Authors and Affiliations

  1. College of Animal Science and Technology, Yangzhou University, 48 Wenhui Road, Yangzhou, 225009, Jiangsu, China

    Zhu Zhu, Ziying Wang, Jiayu Wang, Qingsheng Cao, Hui Yang & Yingying Zhang

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  1. Zhu Zhu
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  2. Ziying Wang
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  3. Jiayu Wang
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  4. Qingsheng Cao
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  5. Hui Yang
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Contributions

Zhu Zhu: methodology, validation, formal analysis, investigation, and data curation. Ziying Wang: methodology, formal analysis, investigation, and writing. Jiayu Wang: methodology and investigation. Qingsheng Cao: investigation and writing — review and editing. Hui Yang: methodology and investigation. Yingying Zhang: validation, formal analysis, Investigation, and data curation.

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Correspondence to Yingying Zhang.

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The animal ethics committee of Yangzhou University approved the experimental protocol used in this study.

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Zhu, Z., Wang, Z., Wang, J. et al. Transcriptomic analysis of lipid metabolism in zebrafish offspring of parental long-term exposure to bisphenol A. Environ Sci Pollut Res 30, 51654–51664 (2023). https://doi.org/10.1007/s11356-023-25844-6

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