Abstract
Microbially induced carbonate precipitation (MICP) has been proven to effectively immobilize Cd2+ and Pb2+ using a single bacterium. However, there is an urgent need for studies of Cd2+ and Pb2+ immobilized by a bacterial consortium. In this study, a stable consortium designated JZ1 was isolated from soil that was contaminated with cadmium and lead, and the dominant genus Sporosarcina (99.1%) was found to have carbonate mineralization function. The results showed that 91.52% and 99.38% of Cd2+ and Pb2+ were mineralized by the consortium JZ1 with 5 g/L CaCl2 at an initial concentration of 5 mg/L Cd2+ and 150 mg/L Pb2+, respectively. The bioprecipitates were characterized using X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDS). Moreover, the kinetic studies indicated that the urea hydrolysis reaction fit well with the Michaelis–Menten equation, and the kinetic parameters Km and Vmax were estimated to be 38.69 mM and 58.98 mM/h, respectively. When the concentration of urea increased from 0.1 to 0.3 M, the mineralization rate increased by 1.58-fold. This study can provide a novel microbial resource for the biomineralization of Cd and Pb in soil and water environments.
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This work was financially supported by the National Key Research and Development Project of China [grant number: 2019YFC1805203], the Natural Science Research Key Projects in Colleges and Universities of Anhui Province [grant number: KJ2019A0203], the National Natural Science Foundation of China [grant number: 41101485], and the Innovation and Entrepreneurship Training Program for College Students [grant numbers: 202010364065 and 202110364087]. National Key Research and Development Project of China,2019YFC1805203,Ting Fan,Natural Science Research Key Projects in Colleges and Universities of Anhui Prov-ince, KJ2019A0203, Ting Fan, National Natural Science Foundation of China, 41101485, Ting Fan
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Ting Fan and Peng Zhang designed the study and wrote the paper; Peng Zhang, Li-yuan Yang, and Xiao-Qiang Liu performed the experiment; Peng Zhang, Hua-ze-yu Sheng and An-qi Qian analyzed the data.
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Zhang, P., Liu, XQ., Yang, Ly. et al. Immobilization of Cd2+ and Pb2+ by biomineralization of the carbonate mineralized bacterial consortium JZ1. Environ Sci Pollut Res 30, 22471–22482 (2023). https://doi.org/10.1007/s11356-022-23587-4
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DOI: https://doi.org/10.1007/s11356-022-23587-4