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Mechanistic insights on the possible protective role of polyphenols extracted from Tamarix aphylla aerial parts against sodium arsenite-induced hepatotoxicity in rats

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Abstract

Arsenic exposure is associated with the induction of hepatotoxicity. Current study was aimed to investigate the hepato-protective ability of polyphenolic components of Tamarix aphylla (TA) ethanolic extract against sodium arsenite (SA)-induced liver injury of rats. Significantly higher quantities of phenolic (318.7±2.5 mgg−1GAE) and flavonoid (250.69 ±3.3 mgg−1QE) contents were present. Inhibitory concentration (IC50) exhibited an excellent potential for antioxidant (IC50= 25.99 μg/mL) assay. High performance liquid chromatography (HPLC) confirmed the existence of myercetin (10.40ppm), sinapic acid (2.131ppm), kaempferol (0.486ppm), caffeic acid (5.094 ppm). Forty-two rats were divided into 7 groups. Group 1 received normal saline (2 mL/kg/day, orally for 21 days), Group 2 received SA (10mg/kg/day for 21 days), and Group 3 received SA alone for 7 days (10mg/kg) and continues with silymarine for 21 days (25mg/kg orally). Group 4, 5, 6 received SA alone for 7 days and continue with TA extract up to 21 days (125mg/kg, 250mg/kg, and 500mg/kg orally) respectively, and Group 7 received TA extract (500mg/kg) for 21 days. SA was administered to all treated groups for 21 days. Treatment with polyphenolic ethanolic extract of TA restored the hepatic indices and oxidative markers in a dose-dependent manner. The upregulation in tumor necrosis factor-α, interleukin-6, and cyclooxygenase-2 upon SA treatment suggesting inflammation was normalized by the treatment of rats. Above mentioned biochemical findings were supported well with histopathological screening. Present findings suggest that TA polyphenolic ethanolic extract could mitigate the oxidative stress and inflammation induced by SA in liver tissue.

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References

  • Abdelgawad A (2017) Tamarix nilotica (Ehrenb) bunge: a review of phytochemistry and pharmacology. J Microb Biochem Technol 9:544–553

    CAS  Google Scholar 

  • Ahmad S, Alam K, Wariss H, Anjum S, Mukhtar M (2014) Ethnobotanical studies of plant resources of Cholistan desert, Pakistan. Int J Sci Res 3:1782–1788

    Google Scholar 

  • Al Aboud D, Baty RS, Alsharif KF, Hassan KE, Zhery AS, Habotta OA, Elmahallawy EK, Amin HK, Moneim AEA, Kassab RB (2020) Protective efficacy of thymoquinone or ebselen separately against arsenic-induced hepatotoxicity in rat. Environ Sci Pollut Res 1–12. https://doi.org/10.1007/s11356-020-10955-1

  • Alam MA, Sagor AT, Tabassum N, Ulla A, Shill MC, Rahman GMS, Hossain H, Reza HM (2018) Caffeic acid rich Citrus macroptera peel powder supplementation prevented oxidative stress, fibrosis and hepatic damage in CCl 4 treated rats. Clin Phytosci 4:14

    Article  Google Scholar 

  • Bashir S, Sharma Y, Irshad M, Gupta SD, Dogra T (2006) Arsenic-induced cell death in liver and brain of experimental rats. Basic Clin Pharmacol Toxicol 98:38–43

    Article  CAS  Google Scholar 

  • Chen C (2016) Sinapic acid and its derivatives as medicine in oxidative stress-induced diseases and aging. Oxid Med Cell Longev. https://doi.org/10.1155/2016/3571614

  • Chen YC, Lin-Shiau SY, Lin JK (1998) Involvement of reactive oxygen species and caspase 3 activation in arsenite-induced apoptosis. J Cell Physiol 177:324–333

    Article  CAS  Google Scholar 

  • Das AK, Bag S, Sahu R, Dua TK, Sinha MK, Gangopadhyay M, Zaman K, Dewanjee S (2010) Protective effect of Corchorus olitorius leaves on sodium arsenite-induced toxicity in experimental rats. Food Chem Toxicol 48:326–335

    Article  CAS  Google Scholar 

  • Dong W, Chen Q, Wei C, Hu R, Long Y, Zong Y, Chu Z (2021) Comparison of the effect of extraction methods on the quality of green coffee oil from Arabica coffee beans: lipid yield, fatty acid composition, bioactive components, and antioxidant activity. Ultrason Sonochem 74:105578

    Article  CAS  Google Scholar 

  • Działo M, Mierziak J, Korzun U, Preisner M, Szopa J, Kulma A (2016) The potential of plant phenolics in prevention and therapy of skin disorders. Int J Mol Sci 17:160

    Article  Google Scholar 

  • El-Aarag B, Khairy A, Khalifa SA, El-Seedi HR (2019) Protective effects of flavone from Tamarix aphylla against CCl4-induced liver injury in mice mediated by suppression of oxidative stress, apoptosis and angiogenesis. Int J Mol Sci 20:5215

    Article  CAS  Google Scholar 

  • El-Demerdash FM, Yousef MI, Radwan FM (2009) Ameliorating effect of curcumin on sodium arsenite-induced oxidative damage and lipid peroxidation in different rat organs. Food Chem Toxicol 47:249–254

    Article  CAS  Google Scholar 

  • El-Shehawi AM, Alkafafy M, El-Shazly S, Sayed S, Farouk S, Alotaibi S, Madkour DA, Khalifa HK, Ahmed MM (2021) Moringa oleifera leaves ethanolic extract ameliorates high fat diet-induced obesity in rats. J King Saud Univ-Sci 33:101552

    Article  Google Scholar 

  • Fatemi I, Khalili H, Mehrzadi S, Basir Z, Malayeri A, Goudarzi M (2021) Mechanisms involved in the possible protective effect of chrysin against sodium arsenite-induced liver toxicity in rats. Life Sci 267:118965

    Article  CAS  Google Scholar 

  • Flora S, Kannan G, Pant B, Jaiswal D (2003) The efficacy of monoisoamyl ester of dimercaptosuccinic acid in chronic experimental arsenic poisoning in mice. J Environ Sci Health A 38:241–254

    Article  CAS  Google Scholar 

  • Flora SJS, Bhadauria S, Pant SC, Dhaked RK (2005) Arsenic induced blood and brain oxidative stress and its response to some thiol chelators in rats. Life Sci 77:2324–2337

    Article  CAS  Google Scholar 

  • Gadallah AS, Yousuf S, Jabeen A, Swilam MM, Khalifa SA, El-Seedi HR, Choudhary MI (2020) Anti-inflammatory principles from Tamarix aphylla L.: a bioassay-guided fractionation study. Molecules 25:2994

    Article  CAS  Google Scholar 

  • Gholamine B, Houshmand G, Hosseinzadeh A, Kalantar M, Mehrzadi S, Goudarzi M (2019) Gallic acid ameliorates sodium arsenite-induced renal and hepatic toxicity in rats. Drug Chem Toxicol 44(4):341–352

    Article  Google Scholar 

  • Gülcin I (2012) Antioxidant activity of food constituents: an overview. Arch Toxicol 86:345–391

    Article  Google Scholar 

  • Habib M, Bhatti S, ur Rehman S, Javed N, Aslam MS, Shahzad N, Abbas Z (2020) Hepatoprotective role of swimming against arsenic induced oxidative stress in mice. J King Saud Univ-Sci 32:822–827

    Article  Google Scholar 

  • Hidalgo M, Sánchez-Moreno C, de Pascual-Teresa S (2010) Flavonoid–flavonoid interaction and its effect on their antioxidant activity. Food Chem 121:691–696

    Article  CAS  Google Scholar 

  • Hou W, Zhang W, Chen G, Luo Y (2016) Optimization of extraction conditions for maximal phenolic, flavonoid and antioxidant activity from melaleuca bracteata leaves using the response surface methodology. PLoS One 11:e0162139

    Article  Google Scholar 

  • Hughes MF (2002) Arsenic toxicity and potential mechanisms of action. Toxicol Lett 133:1–16

    Article  CAS  Google Scholar 

  • Hussain F, Malik A, Ayyaz U, Shafique H, Rana Z, Hussain Z (2017) Efficient hepatoprotective activity of cranberry extract against CCl4-induced hepatotoxicity in Wistar albino rat model: down-regulation of liver enzymes and strong antioxidant activity. Asian Pac J Trop Med 10:1054–1058

    Article  Google Scholar 

  • Khan D, Sharif A, Zafar M, Akhtar B, Akhtar MF, Awan S (2020) Delonix regia a folklore remedy for diabetes; attenuates oxidative stress and modulates type II diabetes mellitus. Curr Pharm Biotechnol 21:1059–1069

    Article  CAS  Google Scholar 

  • Kim Y-J, Kim J-M (2015) Arsenic toxicity in male reproduction and development. Dev Reprod 19:167

    Article  Google Scholar 

  • Kim JK, Park SU (2020) Recent studies on kaempferol and its biological and pharmacological activities. EXCLI J 19:627

    Google Scholar 

  • Lu Y, Wahl LM (2005) Oxidative stress augments the production of matrix metalloproteinase-1, cyclooxygenase-2, and prostaglandin E2 through enhancement of NF-κB activity in lipopolysaccharide-activated human primary monocytes. J Immunol 175:5423–5429

    Article  CAS  Google Scholar 

  • Mahfoudhi A, Prencipe FP, Mighri Z, Pellati F (2014) Metabolite profiling of polyphenols in the Tunisian plant Tamarix aphylla (L.) Karst. J Pharm Biomed Anal 99:97–105

    Article  CAS  Google Scholar 

  • Majhi CR, Khan S, Leo MDM, Manimaran A, Sankar P, Sarkar SN (2011) Effects of acetaminophen on reactive oxygen species and nitric oxide redox signaling in kidney of arsenic-exposed rats. Food Chem Toxicol 49:974–982

    Article  CAS  Google Scholar 

  • Malik M, Sharif A, Hassan SU, Muhammad F, Khan HM, Akhtar B, Saeed M (2020) Amelioration of hyperglycaemia and modulation of pro-inflammatory cytokines by Tamarix gallica fractions in alloxan induced diabetic rats. Arch Physiol Biochem 1–10. https://doi.org/10.1080/13813455.2020.1788099

  • Mangrulkar S, Shah P, Navnage S, Mazumdar P, Chaple D (2021) Phytophospholipid complex of caffeic acid: development, in vitro characterization, and in vivo investigation of antihyperlipidemic and hepatoprotective action in rats. AAPS PharmSciTech 22:1–16

    Article  Google Scholar 

  • Marwat SK, Rehman F (2011) Medicinal folk recipes used as traditional phytotherapies in district Dera Ismail Khan, KPK, Pakistan. Pak J Bot 43:1453–1462

    Google Scholar 

  • Mehrzadi S, Fatemi I, Malayeri AR, Khodadadi A, Mohammadi F, Mansouri E, Rashno M, Goudarzi M (2018) Ellagic acid mitigates sodium arsenite-induced renal and hepatic toxicity in male Wistar rats. Pharmacol Rep 70:712–719

    Article  CAS  Google Scholar 

  • Mittal M, Flora SJS (2006) Effects of individual and combined exposure to sodium arsenite and sodium fluoride on tissue oxidative stress, arsenic and fluoride levels in male mice. Chem Biol Interact 162:128–139

    Article  CAS  Google Scholar 

  • Mohammedi Z, Atik F (2011) Impact of solvent extraction type on total polyphenols content and biological activity from Tamarix aphylla (L.) Karst. Int J Pharma Bio Sci 2(1):609–615

  • Nawwar MA, Hussein SA, Ayoub NA, Hofmann K, Linscheid M, Harms M, Wende K, Lindequist U (2009) Aphyllin, the first isoferulic acid glycoside and other phenolics from Tamarix aphylla flowers. Die Pharm 64:342–347

    CAS  Google Scholar 

  • Ola-Davies OE, Akinrinde AS (2016) Acute sodium arsenite-induced hematological and biochemical changes in wistar rats: protective effects of ethanol extract of Ageratum conyzoides. Pharm Res 8:S26

    Google Scholar 

  • Raeeszadeh M, Karimi P, Khademi N, Mortazavi P (2022) The effect of broccoli extract in arsenic-induced experimental poisoning on the hematological, biochemical, and electrophoretic parameters of the liver and kidney of rats. Evid Based Complement Alternat Med 2022:3509706

    Article  Google Scholar 

  • Saeed I, Guo X, Azeem M, Elshikh MS, Zainab B, Ayaz Z, You L, Alwahibi MS, Abbasi AM (2021) Comparative assessment of polyphenolics’ content, free radicals’ scavenging and cellular antioxidant potential in apricot fruit. J King Saud Univ-Sci 33:101459

    Article  Google Scholar 

  • Saha S, Rashid K, Sadhukhan P, Agarwal N, Sil PC (2016) Attenuative role of mangiferin in oxidative stress-mediated liver dysfunction in arsenic-intoxicated murines. Biofactors 42:515–532

    Article  CAS  Google Scholar 

  • Sharif A, Ashraf M, Anjum AA, Javeed A, Altaf I, Akhtar MF, Abbas M, Akhtar B, Saleem A (2016a) Pharmaceutical wastewater being composite mixture of environmental pollutants may be associated with mutagenicity and genotoxicity. Environ Sci Pollut Res 23:2813–2820

    Article  CAS  Google Scholar 

  • Sharif A, Ashraf M, Javeed A, Anjum AA, Akhtar MF, Akhtar B, Saleem A (2016b) Oxidative stress responses in Wistar rats on subacute exposure to pharmaceutical wastewater. Environ Sci Pollut Res 23:24158–24165

    Article  CAS  Google Scholar 

  • Suleiman MHA (2019) Ethnobotanical, phytochemical, and biological study of Tamarix aphylla and Aerva javanica medicinal plants growing in the Asir Region, Saudi Arabia. Trop Conserv Sci 12:1940082919869480

    Article  Google Scholar 

  • Turk E, Kandemir FM, Yildirim S, Caglayan C, Kucukler S, Kuzu M (2019) Protective effect of hesperidin on sodium arsenite-induced nephrotoxicity and hepatotoxicity in rats. Biol Trace Elem Res 189:95–108

    Article  CAS  Google Scholar 

  • Wang S-J, Tong Y, Lu S, Yang R, Liao X, Xu Y-F, Li X (2010) Anti-inflammatory activity of myricetin isolated from Myrica rubra Sieb. et Zucc. leaves. Planta Med 76:1492–1496

    Article  CAS  Google Scholar 

  • Yang S-C, Sung P-J, Lin C-F, Kuo J, Chen C-Y, Hwang T-L (2014) Anti-inflammatory effects of secondary metabolites of marine Pseudomonas sp. in human neutrophils are through inhibiting P38 MAPK, JNK, and calcium pathways. PLoS One 9:e114761

    Article  Google Scholar 

  • Yousef MI, El-Demerdash FM, Radwan FM (2008) Sodium arsenite induced biochemical perturbations in rats: ameliorating effect of curcumin. Food Chem Toxicol 46:3506–3511

    Article  CAS  Google Scholar 

  • Yusufoglu HS, Alqasoumi SI (2011) Anti-inflammatory and wound healing activities of herbal gel containing an antioxidant Tamarix aphylla leaf extract. Int J Pharmacol 7:829–835

    Article  Google Scholar 

  • Zafar M, Sharif A, Khan D, Akhtar B, Muhammad F, Akhtar MF, Fatima T (2020) Preventive effect of Euphorbia royleana Boiss on diabetes induced by streptozotocin via modulating oxidative stress and deoxyribonucleic acid damage. Toxin Reviews 40(4):777–790

    Article  Google Scholar 

  • Zaib M, Sharif A, Akhtar B, Khan HM, Akhtar MF, Hassan W, Razzaq F, Nawaz S, Qaisar N (2020): Berberis lycium Royle. extracts attenuate inflammation and modulates hyperglycemia in alloxan induced diabetic rats. Pak J Pharm Sci. https://doi.org/10.36721/PJPS.2020.33.4.SUP.1805-1813.1

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Authors and Affiliations

  1. Faculty of Pharmacy, The University of Lahore, Lahore, Pakistan

    Shaher Bano & Faiza Liaqat Ali

  2. Department of Pharmacology, Institute of Pharmacy, Faculty of Pharmaceutical and Allied Health Sciences, Lahore College for Women University, Lahore, Pakistan

    Ali Sharif

  3. Department of Pharmacy, University of Agriculture, Faisalabad, Pakistan

    Bushra Akhtar

  4. Department of Pharmaceutical Sciences, Pharmacy Program, Batterjee Medical College, P.O. Box 6231, Jeddah, 21442, Saudi Arabia

    Mohamed M. Abdel-Daim

  5. Pharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt

    Mohamed M. Abdel-Daim

  6. Riphah Institute of Pharmaceutical Sciences, Riphah International University, Lahore Campus, Lahore, Pakistan

    Muhammad Furqan Akhtar

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  1. Shaher Bano
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Contributions

All authors contributed to the study conception, design, and writing of manuscript. Material preparation and data collection were performed by SB and FLA. Methodology design was devised by MFA. BA helped in conceptualization and validation of the study. The first draft of the manuscript was written by AS along with project administration. MMA helped in editing and reviewing of the original draft. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Ali Sharif.

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The study was conducted followed by the approval of the Animal Ethical Committee (IREC-2019-87) in accordance with the NC3Rs ARRIVE Guidelines, adhering to ethical guidelines of The Basel Declaration, the International Council for Laboratory Animal Science (ICLAS) ethical guidelines, and Directive 2010/63/EU.

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Bano, S., Sharif, A., Akhtar, B. et al. Mechanistic insights on the possible protective role of polyphenols extracted from Tamarix aphylla aerial parts against sodium arsenite-induced hepatotoxicity in rats. Environ Sci Pollut Res 30, 16565–16578 (2023). https://doi.org/10.1007/s11356-022-23324-x

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