Abstract
In this study, the toxicity of epichlorohydrin, a chemical intermediate, was investigated by using Allium cepa L. test material as a bio-indicator. In addition, the protective role of sage leaf extract (Slex) against this toxicity was investigated. Toxicity was handled with the help of physiological (germination percentage, root elongation, and weight gain), cytogenetic (mitotic index = MI, micronucleus = MN, and chromosomal abnormalities = CAs), biochemical (malondialdehyde = MDA, superoxide dismutase = SOD, and catalase = CAT), and anatomical (root meristem cell damages) parameters. A. cepa bulbs were divided into 6 groups (1 control, 5 applications). The bulbs in the control group were treated with tap water, and the bulbs in the application group were treated with epichlorohydrin at a dose of 100 mg/L and Slex at two different doses (190 mg/L and 380 mg/L) and germinated. Germination process was continued uninterruptedly for 72 h in all groups. At the end of the period, physiological parameter measurements were carried out in the bulbs. In addition, root tips were collected and made ready for cytogenetic, biochemical, and anatomical measurements and microscopic observations. As a result, exposure to epichlorohydrin caused statistically significant (p < 0.05) decreases in germination percentage, root length, weight gain, and MI, and statistically significant (p<0.05) increases in MN frequency, CA numbers, MDA level, SOD, and CAT enzyme activities. Epichlorohydrin exposure induced CAs such as fragment, sticky chromosome, unequal distribution of chromatin, reverse polarization, and disordered mitosis in root meristem cells. The toxicity of epichlorohydrin was due to its interaction with DNA, and this interaction was confirmed by the spectral shift in the DNA spectrum. In addition, epichlorohydrin caused anatomical damages such as epidermis cell damage, cortex cell damage, thickening of the cortex cell wall, and flattened cell nuclei in root meristem cells. The application of Slex together with epichlorohydrin decreased the toxicity of epichlorohydrin and again caused statistically significant (p < 0.05) improvements in the values of all the parameters examined. In other words, germination percentage, root length, weight gain, and MI increased again and MN frequency, CAs numbers, MDA level, SOD, and CAT enzyme activities decreased. It was determined that this improvement was even more pronounced at 380 mg/L dose of Slex. As a result, it was determined that epichlorohydrin caused multiple-toxicity for the investigated indicator organism, and Slex had a reducing role in this toxicity. For this reason, Slex should be included in the daily diet as an antioxidant beverage in order to protect from the toxicity of chemical agents exposed in daily life or to reduce their effects.
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References
Acar A, Türkmen Z, Çavuşoğlu K, Yalçın E (2020) Investigation of benzyl benzoate toxicity with anatomical, physiological, cytogenetic and biochemical parameters in vivo. Caryologia 73(3):21–32. https://doi.org/10.13128/caryologia-167
Ackerman MM, Ricciardi C, Weiss D, Chant A, Kraemer-Chant CM (2016) Analyzing exonuclease-induced hyperchromicity by UV spectroscopy: an undergraduate biochemistry laboratory experiment. J Chem Educ 93(12):2089–2095. https://doi.org/10.1021/acs.jchemed.6b00095
Ahmed ES, Shoman TM, Ezz-Eldin A, Ahmed KA (2010) Protective effect of sage (Salvia officinalis L.) on cyclophosphamide toxicity evaluated by cytogenetic, biochemical and histopathological assays in male albino mice. Egypt J Comp Path Clinic Path 23(2):121–137
Akgündüz MÇ, Çavuşoğlu K, Yalçın E (2020) The potential risk assessment of phenoxyethanol with a versatile model system. Sci Rep 10:1209. https://doi.org/10.1038/s41598-020-58170-9
Alshubaily FA, Jambi EJ (2018) The possible protective effect of sage (Salvia Officinalis L.) water extract against testes and heart tissue damages of hypercholesterolemic rats. Int J Pharm Phytopharm Res 8(1):62–68
Atik M, Karagüzel O, Ersoy S (2007) Effect of temperature on germination characteristics of Dalbergia sissoo seeds. Mediterr Agric Sci 20:203–208
Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to Acrylamide gels. Anal Biochem 44(1):276–287. https://doi.org/10.1016/0003-2697(71)90370-8
Beers RF, Sizer IW (1952) Colorimetric method for estimation of catalase. J Biol Chem 195:133–139
Çavuşoğlu D, Yalçin E, Çavuşoğlu K, Acar A, Yapar K (2021) Molecular docking and toxicity assessment of spirodiclofen: protective role of lycopene. Environ Sci Pollut Res 28(40):57372–57385. https://doi.org/10.1007/s11356-021-14748-y
Çavuşoğlu K, Kurt D, Yalçın E (2020) A versatile model for investigating the protective effects of Ceratonia siliqua pod extract against 1,4-dioxane toxicity. Environ Sci Pollut Res 27:27885–27892. https://doi.org/10.1007/s11356-020-08545-2
Çavuşoğlu K, Yalçın E, Acar A (2022) Protective role of Salvia officinalisis against paraquat toxicity in Swiss albino mice: a genetic approach. International Aegean Conferences on Natural & Medical Sciences V 171-180, İzmir-Turkey
Demirtaş G, Çavuşoğlu K, Yalçın E (2020) Aneugenic, clastogenic, and multi-toxic effects of diethyl phthalate exposure. Environ Sci Pollut Res 27(5):5503–5510. https://doi.org/10.1007/s11356-019-07339-5
Di Cesare E, Verrico A, Miele A, Giubettini M, Rovella P, Coluccia A, Famiglini V, La Regina G, Cundari E, Silvestri R, Lavia P (2017) Mitotic cell death induction by targeting the mitotic spindle with tubulin-inhibitory indole derivative molecules. Oncotarget 8(12):19738. https://doi.org/10.18632/oncotarget.14980
Duke SO, Lydon J, Koskinen WC, Moorman TB, Chaney RL, Hammerschmidt R (2012) Glyphosate effects on plant mineral nutrition, crop rhizosphere microbiota, and plant disease in glyphosate-resistant crops. J Agric Food Chem 60(42):10375–10397. https://doi.org/10.1021/jf302436u
European Commission (2011) Recommendation from the scientific committee on occupational exposure limits for epichlorohydrin. https://ec.europa.eu/social/BlobServlet?langId=en&docId=7304&. Accessed 22 June 2022.
Fenech M, Chang WP, Kirsch-Volders M, Holland N, Bonassi S, Zeiger E (2003) HUMN Project: detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures. Mutat Res 534(1):65–75. https://doi.org/10.1016/S1383-5718(02)00249-8
Hayashi K, Hasegawa J, Matsunaga S (2013) The boundary of the meristematic and elongation zones in roots: endoreduplication precedes rapid cell expansion. Sci Rep 3(1):1–8. https://doi.org/10.1038/srep02723
Ighodaro OM, Akinloye OA (2018) First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): their fundamental role in the entire antioxidant defence grid. Alexandria J Med 54(4):287–293. https://doi.org/10.1016/j.ajme.2017.09.001
Kurt D, Acar A, Çavuşoğlu D, Yalçın E, Çavuşoğlu K (2021) Genotoxic effects and molecular docking of 1,4-dioxane: combined protective effects of trans-resveratrol. Environ Sci Pollut Res 28(39):54922–54935. https://doi.org/10.1007/s11356-021-14387-3
Marnett LJ (1999) Lipid peroxidation-DNA damage by malondialdehyde. Mutat Res 424(1–2):83–95. https://doi.org/10.1016/s0027-5107(99)00010-x
Mithöfer A, Maffei ME (2017) General mechanisms of plant defense and plant toxins. In: Gopalakrishnakone P, Carlini C, Ligabue-Braun R (eds) Plant toxins, Toxinology. Springer, Dordrecht, pp 3–24
Mohammed HA, Eldeeb HM, Khan RA, Al-Omar MS, Mohammed SA, Sajid MS, Aly MSA, Ahmad AM, Abdellatif AAH, Eid SY, El-Readi MZ (2021) Sage, Salvia officinalis L., constituents, hepatoprotective activity, and cytotoxicity evaluations of the essential oils obtained from fresh and differently timed dried herbs: a comparative analysis. Molecules 26(19):5757. https://doi.org/10.3390/molecules26195757
Lawrence WH, Malik M, Turner JE, Autian J (1972) Toxicity profile of epichlorohydrin. J Pharm Sci 61(11):1712–1717. https://doi.org/10.1002/jps.2600611103
Lopresti AL (2017) Salvia (Sage): a review of its potential cognitive-enhancing and protective effects. Drugs R D 17(1):53–64. https://doi.org/10.1007/s40268-016-0157-5
Porte A, Godoy RLO, Maia-Porte LH (2013) Chemical composition of sage (Salvia officinalis L.) essential oil from the Rio de Janeiro State (Brazil). Rev Bras Plantas Med 15:438–441
Qian XW (2004) Mutagenic effects of chromium trioxide on root tip cells of Vicia faba. J Zhejiang Univ Sci A 12(5):1570–1576. https://doi.org/10.1631/jzus.2004.1570
Romano KP, Newman AG, Zahran RW Millard JT (2007) DNA interstrand cross-linking by epichlorohydrin. Chem Res Toxicol 20:832-838. https://doi.org/10.1021/tx700066h.
Samiei S, Khadem M, Pourbabaki R, Amirkhanlou F, Shahtaheri SJ (2020) Effect of Salvia officinalis extract (sage) on the toxicity and oxidative stress of deltamethrin in kidneys of the rats. J Occup Hyg Eng 7(3):36–46. https://doi.org/10.52547/johe.7.3.36
Sharma AD, Gill PK, Singh P (2002) DNA isolation from dry and fresh samples of polysaccharide-rich plants. Plant Mol Biol Report 20:415. https://doi.org/10.1007/BF02772129
Singh Z, Karthigesu IP, Singh P, Rupinder K (2014) Use of malondialdehyde as a biomarker for assessing oxidative stress in different disease pathologies: a review. Iran J Public Health 43(3):7–16
Singh S, Parihar P, Singh R, Singh VP, Prasad SM (2016) Heavy metal tolerance in plants: role of transcriptomics, proteomics, metabolomics, and ionomics. Front Plant Sci 6:1143. https://doi.org/10.3389/fpls.2015.01143
Singh US, Secker-Samuelian K, Solomon JJ (1996) Reaction of epichlorohydrin with 2’deoxynucleosides: cheracterization of adducts. Chem Biol Interact 99:109–128. https://doi.org/10.1016/0009-2797(95)03665-2
Staykova TA, Ivanova EN, Velcheva IG (2005) Cytogenetic effect of heavy-metal and cyanide in contaminated waters from the region of southwest Bulgaria. J Cell Mol Biol 4:41–46
Unyayar S, Celik A, Cekic FO, Gozel A (2006) Cadmium-induced genotoxicity, cytotoxicity and lipid peroxidation in Allium sativum and Vicia faba. Mutagenesis 21:77–81. https://doi.org/10.1093/mutage/gel001
Yalçın E, Uzun A, Çavuşoğlu K (2019) In vivo epiclorohidrine toxicity: cytogenetic, biochemical, physiological and anatomical evidences. Environ Sci Pollut Res 26:22400–22406. https://doi.org/10.1093/mutage/gel001.10.1007/s11356-019-05518-y
Yactayo-Chang JP, Tang HV, Mendoza J, Christensen SA, Block AK (2020) Plant defense chemicals against insect pests. Agronomy 10(8):1156. https://doi.org/10.3390/agronomy10081156
Zou J, Yue J, Jiang W, Liu D (2012) Effects of cadmium stress on root tip cells and some physiological indexes in Allium cepa var. agrogarum L. Acta Biol Cracov Ser Bot 54(1):129–141. https://doi.org/10.2478/v10182-012-0015-x
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Çavuşoğlu, K., Yalçin, E. Spectral shift supported epichlorohydrin toxicity and the protective role of sage. Environ Sci Pollut Res 30, 1374–1385 (2023). https://doi.org/10.1007/s11356-022-22288-2
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DOI: https://doi.org/10.1007/s11356-022-22288-2