Abstract
Edible amaranth (Amaranthus tricolor L.) is used as a food-medicine or ornamental plant, and despite its importance, there are few reports associated with cadmium (Cd) stress. This study aimed to appraise the crosstalk between sodium nitroprusside (SNP), as a source of nitric oxide (NO), and cadmium toxicity on growth and physiological traits in edible amaranth by using different multivariate statistical methods. The results showed that growth-related traits of A. tricolor were significantly reduced under Cd stress. Contrarily, Cd treatments increased lipid peroxidation and reduced total protein content. Delving on the results of SNP application showed the suitability of its medium level (100 µM) on increasing the growth-related traits and also plant tolerance to Cd stress via lowering the lipid peroxidation and radical molecules production due to the higher activities of superoxide dismutase and catalase. Increasing the amount of Cd in roots and shoots, as the result of Cd treatment, reduced the growth and production of A. tricolor plants by high rates (over 50% in 60 mg kg−1 Cd level), indicating its susceptibility to high Cd toxicity. Contrarily, treating plants with SNP showed no effect on shoot Cd content, while it significantly increased Cd allocation in the root, which might be attributable to the protective effect of NO on Cd toxicity by trapping Cd in the root. Subsequently, the application of a medium level of SNP (around 100 µM) is recommendable for A. tricolor plant to overcome the negative impacts of Cd toxicity. Moreover, according to the results of heatmap and biplot, under no application of Cd, the application of 100 µM SNP showed a great association with growth-related traits indicating the effectiveness of SNP on the productivity of this species even under no stress situations.
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Abbreviations
- Cd-soil:
-
Cadmium content in soil
- Cd-rt:
-
Cadmium content in shoot
- TF:
-
Translocation factors
- T-phen:
-
Total phenol
- Cart:
-
Carotenoid content
- Rl:
-
Root length
- Shl:
-
Shoot length
- RDW:
-
Root dry weight
- SDW:
-
Shoot dry weight
- H2O2 :
-
Hydrogen peroxidase
- MDA:
-
Malondialdehyd
- SOD:
-
Superoxide dismutase
- CAT:
-
Catalyze
- POD:
-
Peroxidase
- SNP:
-
Sodium nitroprusside
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In this manuscript, FB carried out the experiment and performed the main parts in the data gathering, analyzing, and writing the manuscript. MA cooperated in performing the experiment and data gathering along with the manuscript writing. VRS and MM edited the manuscript scientifically. The grammatical corrections and structural editing of the final manuscript were carried out by FB and VRS.
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Baniasadi, F., Arghavani, M., Saffari, V.R. et al. Multivariate analysis of morpho-physiological traits in Amaranthus tricolor as affected by nitric oxide and cadmium stress. Environ Sci Pollut Res 29, 49092–49104 (2022). https://doi.org/10.1007/s11356-022-19430-5
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DOI: https://doi.org/10.1007/s11356-022-19430-5