Abstract
Many studies have demonstrated the effectiveness of algicidal compounds produced by macrophytes against microalgae. The aim of this study was to assess the algicidal activity of seven Moroccan macrophyte ethyl acetate extracts (MEA) to control harmful algal blooms (HABs). The response and sensitivity of prokaryotic toxic cyanobacteria (Microcystis aeruginosa) and eukaryotic microalgae (Chlorella sp.) were highlighted. The algicidal effect of MEA extracts against the two microalgae was assessed using both the paper disc diffusion and microdilution methods. This last was used in order to evaluate the minimum inhibitory concentrations (MIC) and minimum algicidal concentrations (MAC). Results showed that the growth of both microalgae was significantly inhibited by all MEA extracts. Myriophyllum spicatum organic extract shows the highest growth inhibition activity against M. aeruginosa (35.33 ± 1.53) and Chlorella sp. (30.33 ± 1.15 mm). This stronger inhibitory activity was confirmed by the low MIC (6.25, 12.5 mg/L) and MAC (6.25, 12.5 mg/L) values. Furthermore, results showed different sensitivity between the prokaryotic and eukaryotic microalgae into MEA extracts. Based on the MIC and MAC values, we can distinguish two groups of plants. The first one, including M. spicatum, Ranunculus aquatilis, and Enteromorpha sp., can be considered as a preferable anti-prokaryotic group with a stronger inhibitory activity on M. aeruginosa growth. The second group, constituted by Potamogeton natans, Nasturtium officinale, Elodea sp., and Ceratophyllum sp., has a preferable and stronger inhibitory effect against eukaryotic algae (Chlorella sp.). Overall the results reveal the potential algicidal activity of macrophytes and suggested that MEA extracts could play an important role in biocontrol of HABs.
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Acknowledgments
This work was supported by the Laboratory of Water, Biodiversity, and Climate Change Laboratory. Phycology, Biotechnology, and Environmental Toxicology Research Unit. The authors thank Hassan Benaissa and Hanane Rassam for helping in the macrophytes sampling.
The useful comments of anonymous reviewers are also acknowledged.
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Tazart, Z., Douma, M., Caldeira, A.T. et al. Highlighting of the antialgal activity of organic extracts of Moroccan macrophytes: potential use in cyanobacteria blooms control. Environ Sci Pollut Res 27, 19630–19637 (2020). https://doi.org/10.1007/s11356-020-08440-w
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DOI: https://doi.org/10.1007/s11356-020-08440-w