Abstract
In dryland cropping ecosystems, nitrogen (N) fertilization causes changes in soil biochemical cycling and influences the crop biomass and grain yield. Based on a long-term field experiment involving three N rates (0 (N0), 160 (N160), and 320 (N320) kg N ha−1 year−1), we measured nitrous oxide (N2O) and carbon dioxide (CO2) emissions from wheat fields and determined the soil organic carbon (SOC), total N, microbial biomass C (MBC), and N (MBN), mineralized C (Cmin) and N (Nmin) concentrations and the bacterial community composition. The results showed that N fertilization significantly (P < 0.05) increased N2O and CO2 emissions. N fertilization also significantly (P < 0.05) increased SOC by 13.2%, total N by 7.4%, MBN by 34.3%, Cmin by 15.5%, and Nmin by 27.8%, as well as the soil bacterial diversity and abundances of N cycling functional groups (by 149%). Additionally, N2O and CO2 emissions were positively and linearly correlated with NH4-N and NO3-N concentrations and positively correlated with SOC, total N, MBN, and the number of bacterial species, as well as most of the bacterial phyla and functional groups. These results suggest that N fertilizer applied to the dryland soils improved soil fertility and changed the soil bacterial community composition and function, thus increased N2O and CO2 emissions. Therefore, to mitigate greenhouse gas emissions and maintain soil fertility for wheat production, an optimized N fertilizer rate should be applied in the southern region of the Loess Plateau in China.
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References
Alvarez R (2005) A review of nitrogen fertilizer and conservation tillage effects on soil organic carbon storage. Soil Use Manag 21(1):38–52. https://doi.org/10.1079/SUM2005291
Bardgett RD, Freeman C, Ostle NJ (2008) Microbial contributions to climate change through carbon cycle feedbacks. ISME J 2(8):805–814. https://doi.org/10.1038/ismej.2008.58
Berthrong ST, Yeager CM, Gallegos-Graves L, Steven B, Eichorst SA, Jackson RB (2014) Nitrogen fertilization has a stronger effect on soil nitrogen-fixing bacterial communities than elevated atmospheric CO2. Appl Environ Microbiol 80(10):3103–3112. https://doi.org/10.1128/AEM.04034-13
Bokulich NA, Kaehler BD, Rideout JR, Dillon M, Bolyen E, Knight R, Huttley GA, Gregory Caporaso J (2018) Optimizing taxonomic classification of marker-gene amplicon sequences with QIIME 2’s q2-feature-classifier plugin. Microbiome 6:90. https://doi.org/10.1186/s40168-018-0470-z
Bolyen E, Rideout JR, Dillon MR et al (2019) Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2. Nat Biotechnol 37:852–857. https://doi.org/10.1038/s41587-019-0209-9
Bremner JM, Mulvaney CS (1982) Nitrogen-total. In: Page AL, Miller RH, Keeney DR (eds) Methods of soil analysis. Part 2. Chemical and microbiological properties. American Society of Agronomy Inc., Madison, pp 595–624
Brookes P, Kragt J, Powlson D, Jenkinson D (1985) Chloroform fumigation and the release of soil nitrogen: the effects of fumigation time and temperature. Soil Biol Biochem 17(6):831–835. https://doi.org/10.1016/0038-0717(85)90143-9
Bundy L, Meisinger J (1994) Nitrogen availability indices. In: Weaver R, Angle S, Bottomley P, Bezdicek D, Smith S, Tabatabai A, Wollum A (eds) Methods of soil analysis, part 2. Microbiological and biochemical properties. Soil Science Society of America, Madison, pp 951–984
Cederlund H, Wessén E, Enwall K, Jones CM, Juhanson J, Pell M (2014) Soil carbon quality and nitrogen fertilization structure bacterial communities with predictable responses of major bacterial phyla. Appl Soil Ecol 84:62–68. https://doi.org/10.1016/j.apsoil.2014.06.003
Conrad R (1996) Soil microorganisms as controllers of atmospheric trace gases (H2, CO, CH4, OCS, N2O, and NO). Microbiol Rev 60(4):609–640. https://doi.org/10.1006/mpat.1996.0079
Dai J, Wang Z, Li F, He G, Wang S, Li Q (2015) Optimizing nitrogen input by balancing winter wheat yield and residual nitrate-N in soil in a long-term dryland field experiment in the Loess Plateau of China. Field Crop Res 181:32–41. https://doi.org/10.1016/j.fcr.2015.06.014
Dong Z, Zhu B, Hua K, Jiang Y (2015) Linkage of N2O emissions to the abundance of soil ammonia oxidizers and denitrifiers in purple soil under long-term fertilization. Soil Sci Plant Nutr 61(5):799–807. https://doi.org/10.1080/00380768.2015.1049930
Fierer N, Bradford MA, Jackson RB (2007) Toward an ecological classification of soil bacteria. Ecology 88(6):1354–1364. https://doi.org/10.1890/05-1839
Firestone MK, Firestone RB, Tiedje JM (1980) Nitrous oxide from soil denitrification: factors controlling its biological production. Science 208(4445):749–751. https://doi.org/10.1126/science.208.4445.749
Franzluebbers K, Weaver R, Juo A, Franzluebbers A (1994) Carbon and nitrogen mineralization from cowpea plants part decomposing in moist and in repeatedly dried and wetted soil. Soil Biol Biochem 26(10):1379–1387. https://doi.org/10.1016/0038-0717(94)90221-6
Gao Q, Hasselquist NJ, Palmroth S, Zheng Z, You W (2014) Short-term response of soil respiration to nitrogen fertilization in a subtropical evergreen forest. Soil Biol Biochem 76(1):297–300. https://doi.org/10.1016/j.soilbio.2014.04.020
Ghosh A, Bhattacharyya R, Dwivedi BS, Meena MC, Agarwal BK, Mahapatra P (2016) Temperature sensitivity of soil organic carbon decomposition as affected by long-term fertilization under a soybean based cropping system in a sub-tropical Alfisol. Agric Ecosyst Environ 233:202–213. https://doi.org/10.1016/j.agee.2016.09.010
Gong ZT, Zhang GL, Luo GB (1999) Diversity of anthrosols in China. Pedosphere 9(3):193–204
Guo J, Liu X, Zhang Y, Shen J, Han W, Zhang W (2010) Significant acidification in major Chinese croplands. Science 327(5968):1008–1010. https://doi.org/10.1126/science.1182570
Hayatsu M, Tago K, Saito M (2008) Various players in the nitrogen cycle: diversity and functions of the microorganisms involved in nitrification and denitrification. Soil Sci Plant Nutr 54(1):33–45. https://doi.org/10.1111/j.1747-0765.2007.00195.x
Haynes RJ (1986) Mineral nitrogen in the plant-soil system. Soil Sci 144(4):302–303. https://doi.org/10.1097/00010694-198710000-00010
Högberg P, Nordgren A, Buchmann N, Taylor A, Ekblad A, Högberg M (2001) Large-scale forest girdling shows that current photosynthesis drives soil respiration. Nature. 411(6839):789–792. https://doi.org/10.1038/35081058
Hu T (2014) Study on GHG emission and mitigation in winter wheat/summer fallow region of south Loess Plateau. Northwest A&F University, Yangling
Hutchinson GL, Mosier AR (1981) Improved soil cover method for field measurement of nitrous oxide fluxes. Soil Sci Soc Am J 45:311–316. https://doi.org/10.2136/sssaj1981.03615995004500020017x
IPCC, (2014) Climate change 2014: synthesis report in: contribution of Working Groups I, II and III to the fifth assessment report of the Intergovernmental Panel on Climate Change [Core Writing Team, RK Pachauri and LA Meyer (eds.)]. IPCC, Geneva
IUSS Working Group WRB. (2015) World Reference Base for soil resources 2014, update 2015. International soil classification system for naming soils and creating legends for soil maps. World soil resources reports no. 106Rep, Rome
Katoh K, Misawa K, Kuma K-i, Miyata T (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Res 30:3059–3066. https://doi.org/10.1093/nar/gkf436
Kim C, Jeong J, Bolan NS, Naidu R (2012) Short-term effects of fertilizer application on soil respiration in red pine stands. J Ecol Environ 35(4):307–311. https://doi.org/10.5141/JEFB.2012.036
Kumar PS, Brooker MR, Dowd SE, Camerlengo T (2011) Target region selection is a critical determinant of community fingerprints generated by 16S pyrosequencing. PLoS One 6(6):e20956. https://doi.org/10.1371/journal.pone.0020956
Lal R (2004) Soil carbon sequestration to mitigate climate change. Geoderma. 123(1–2):1–22. https://doi.org/10.1016/j.geoderma.2004.01.032
Liu J, Dai J, Wang Z, Zhai B (2016) Effects of fallow or planting wheat (Triticum aestivum L.) and fertilizing P or fertilizing P and N practices on soil carbon and nitrogen in a low-organic-matter soil. Soil Sci Plant Nutr 62(3):263–270. https://doi.org/10.1080/00380768.2016.1179559
Livesley SJ, Idczak D, Fest BJ (2013) Differences in carbon density and soil CH4/N2O flux among remnant and agro-ecosystems established since European settlement in the Mornington Peninsula, Australia. Sci Total Environ 465(6):17–25. https://doi.org/10.1016/j.scitotenv.2013.06.042
Louca S, Parfrey LW, Doebeli M (2016) Decoupling function and taxonomy in the global ocean microbiome. Science 353(6305):1272–1277. https://doi.org/10.1126/science.aaf4507
Martin M (2010) Cutadapt removes adapter sequences from high-throughput sequencing reads. EMBnet 17(1):10–12. https://doi.org/10.14806/ej.17.1.200
Müller C, Laughlin RJ, Spott O, Rütting T (2014) Quantification of N2O emission pathways via a 15N tracing model. Soil Biol Biochem 72(1–2):44–54. https://doi.org/10.1016/j.soilbio.2014.01.013
Nelson DW, Sommers LE (1982) Total carbon, organic carbon, and organic matter. In: Page AL (ed) Methods of soil analysis. Part 2. Chemical and microbiological properties-agronomy monograph. American Society of Agronomy, Soil Science Society of America, Madison, pp 539–579
Nielsen UN, Ayres E, Wall DH, Bardgett RD (2011) Soil biodiversity and carbon cycling: a review and synthesis of studies examining diversity–function relationships. Eur J Soil Sci 62(1):105–116. https://doi.org/10.1111/j.1365-2389.2010.01314.x
Paungfoo-Lonhienne C, Yeoh YK, Kasinadhuni NR, Lonhienne TG, Robinson N, Hugenholtz P (2015) Nitrogen fertilizer dose alters fungal communities in sugarcane soil and rhizosphere. Sci Rep 5:8678. https://doi.org/10.1038/srep08678
Pei SW, Zhang YY, Liu JF, Lun XX, Mu YJ (2012) N2O exchange fluxes from wheat-maize crop rotation system in the North China Plain. Huan Jing Ke Xue 33(10):3641–3646 (in Chinese with English abstract)
Price MN, Dehal PS, Arkin AP (2010) FastTree 2–approximately maximum-likelihood trees for large alignments. PLoS One 5:e9490. https://doi.org/10.1371/journal.pone.0009490
Quast C, Pruesse E, Yilmaz P, Gerken J, Schweer T, Yarza P, Peplies J, Gloeckner FO (2013) The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Res 41:D590–D596. https://doi.org/10.1093/nar/gks1219
Rognes T, Flouri T, Nichols B, Quince C, Mahé F (2016) VSEARCH: a versatile open source tool for metagenomics. PeerJ 4:e2584. https://doi.org/10.7717/peerj.2584
Sainju UM, Senwo ZN, Nyakatawa EZ, Tazisong IA, Reddy KC (2008) Soil carbon and nitrogen sequestration as affected by long-term tillage, cropping systems, and nitrogen fertilizer sources. Agric Ecosyst Environ 127(3):234–240. https://doi.org/10.1016/j.agee.2008.04.006
Salinas-Garcia JR, Hons FM, Matocha JE, Zuberer DA (1997) Soil carbon and nitrogen dynamics as affected by long-term tillage and nitrogen fertilization. Biol Fert Soils 25:182–188. https://doi.org/10.1007/s003740050301
Senbayram M, Chen R, Budai A, Bakken L, Dittert K (2012) N2O emission and the N2O/(N2O+N2) product ratio of denitrification as controlled by available carbon substrates and nitrate concentrations. Agric Ecosyst Environ 147(1):4–12. https://doi.org/10.1016/j.agee.2011.06.022
Sharifi M, Zebarth BJ, Burton DL, Grant CA, Bittman S, Drury CF (2008) Response of potentially mineralizable soil nitrogen and indices of nitrogen availability to tillage system. Soil Sci Soc A J 72(4):1124–1131. https://doi.org/10.2136/sssaj2007.0243
Smith KA, Conen F (2004) Impacts of land management on fluxes of trace greenhouse gases. Soil Use Manag 20(2):255–263. https://doi.org/10.1079/SUM2004238
Soares JR, Cassman NA, Kielak AM, Pijl A, Carmo JB, Lourenço KS (2016) Nitrous oxide emission related to ammonia-oxidizing bacteria and mitigation options from N fertilization in a tropical soil. Sci Rep 6:30349. https://doi.org/10.1038/srep30349
Stanford G, Smith SJ (1972) Nitrogen mineralization potentials of soils. Soil Sci Soc A J 36(3):465–472. https://doi.org/10.2136/sssaj1972.03615995003600030029x
Tong C, Xiao H, Tang G, Wang H, Huang T, Xia H (2009) Long-term fertilizer effects on organic carbon and total nitrogen and coupling relationships of C and N in paddy soils in subtropical China. Soil Tillage Res 106(1):8–14. https://doi.org/10.1016/j.still.2009.09.003
Torbert H, Prior S, Reeves D (1999) Land management effects on nitrogen and carbon cycling in an Ultisol. Commun Soil Sci Plant Anal 30(9–10):1345–1349. https://doi.org/10.1080/00103629909370290
Vance ED, Brookes PC, Jenkinson DS (1987) An extraction method for measuring soil microbial biomass C. Soil Biol Biochem 19(6):703–707. https://doi.org/10.1016/0038-0717(87)90052-6
Wang YY, Hu CS (2011) Soil greenhouse gas emission in winter wheat/summer maize rotation ecosystem as affected by nitrogen fertilization in the Piedmont Plain of Mount Taihang, China. Chin J Eco-Agric 19:1122–1128 (in Chinese with English abstract). https://doi.org/10.3724/SP.J.1011.2011.01122
Wang H, Liu J, Hui X, Dai J, Wang Z (2016) Responses of soil organic carbon, organic nitrogen and nitrogen supply capacity to long-term nitrogen fertilization practices in dryland soil. Sci Agric Sin 49(15):2988–2998 (in Chinese with English abstract)
Watts D, Torbert H, Prior S (2007) Mineralization of nitrogen in soils amended with dairy manure as affected by wetting/drying cycles. Commun Soil Sci Plant Anal 38(15–16):2103–2116. https://doi.org/10.1080/00103620701548860
Wei XR, Hao MD, Xue XH, Shi P, Horton R, Wang A (2010) Nitrous oxide emission from highland winter wheat field after long-term fertilization. Biogeosciences 7(10):4539–4563. https://doi.org/10.5194/bgd-7-4539-2010
Wu JS, Joergensen RG, Pommerening B, Chaussod R, Brookes PC (1990) Measurement of soil microbial biomass C by fumigation-extraction—an automated procedure. Soil Biol Biochem 22(8):1167–1169. https://doi.org/10.1016/0038-0717(90)90046-3
Yang XY, Ren WD, Sun BH, Zhang SL (2012) Effects of contrasting soil management regimes on total and labile soil organic carbon fractions in a loess soil in China. Geoderma. 177:49–56. https://doi.org/10.1016/j.geoderma.2012.01.033
Yu Y, Ga M, Zhu B (2012) Comparison study on N2O emissions from field under wheat/maize rotation system and field under vegetable cultivation. Acta Pedol Sin 49(1):96–103 (In Chinese with English abstract)
Zhao HB, Wang ZH, Gao YJ, Zhang WF (2016) Investigation and evaluation of household wheat fertilizer application in Shaanxi Province. J Plant Nutrit Fert 22:245–253 (in Chinese with English abstract)
Zhong Y, Yan WM, Shangguan ZP (2015) Impact of long-term N additions upon coupling between soil microbial community structure and activity, and nutrient-use efficiencies. Soil Biol Biochem 91:151–159. https://doi.org/10.1016/j.soilbio.2015.08.030
Zhou Z, Gan Z, Shangguan Z, Zhang F (2013) Effects of long-term repeated mineral and organic fertilizer applications on soil organic carbon and total nitrogen in a semi-arid cropland. Eur J Agron 45:20–26. https://doi.org/10.1016/j.eja.2012.11.002
Zhu Z, Chen D (2002) Nitrogen fertilizer use in China–contributions to food production, impacts on the environment and best management strategies. Nutr Cycl Agroecosyst 63(2):117–127. https://doi.org/10.1023/A:1021107026067
Zou J, Huang Y, Jiang J, Zheng X, Sass RL (2005) A 3-year field measurement of methane and nitrous oxide emissions from rice paddies in China: effects of water regime, crop residue, and fertilizer application. Global Biogeochem Cycl 19(2):153–174. https://doi.org/10.1029/2004GB002401
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This work was financially supported by the National Key Research and Development Program of China (No. 2016YFD0200105 and No. 2018YFD0200401), Chinese Special Fund for Agro-scientific Research in the Public Interest (No. 201503124), and Chinese National Key Basic Research Special Funds (No. 2015BAD23B04).
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Qiu, W., Liu, J., Li, B. et al. N2O and CO2 emissions from a dryland wheat cropping system with long-term N fertilization and their relationships with soil C, N, and bacterial community. Environ Sci Pollut Res 27, 8673–8683 (2020). https://doi.org/10.1007/s11356-019-07534-4
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DOI: https://doi.org/10.1007/s11356-019-07534-4