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Pseudallescheria boydii and Meyerozyma guilliermondii: behavior of deteriogenic fungi during simulated storage of diesel, biodiesel, and B10 blend in Brazil

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Abstract

Due to their renewable and sustainable nature, biodiesel blends boost studies predicting their stability during storage. Besides chemical degradation, biodiesel is more susceptible to biodegradation due to its raw composition. The aim of this work was to evaluate the deteriogenic potential (growth and degradation) of Pseudallescheria boydii and Meyerozyma guilliermondii in degrading pure diesel (B0), pure biodiesel (B100), and a B10 blend in mineral medium during storage. The biodeterioration susceptibility at different fuel ratios and in BH minimal mineral medium were evaluated. The biomass measurements of P. boydii during 45 days indicated higher biomass production in the B10 blend. The growth curve of M. guilliermondii showed similar growth in B10 and B100. Although there was no significant production of biosurfactant, lipase production was detected in the tributyrin agar medium of both microorganisms. The main compounds identified in the aqueous phase by GC-MS were alcohols, esters, acids, sulfur, ketones, and phenols. The results showed that P. boydii grew at the expense of fuels, degrading biodiesel esters, and diesel hydrocarbons. M. guilliermondii grew in B100 and B10; however, degradation was not detected.

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Acknowledgements

The authors wish to thank LAB-BIO/UFRGS, Chemistry Institute/UFRGS, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the funds provided during the course of this study.

Funding

This study was funded by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

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Authors and Affiliations

  1. LABBIO Biodeterioration of Fuel and Biofuel Laboratory, Institute of Basic Health Sciences, Department of Microbiology, Immunology and Parasitology, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, 500, Porto Alegre, RS, 90050170, Brazil

    Gabriela Boelter, Juciana Clarice Cazarolli, Sabrina Anderson Beker, Patrícia Dörr de Quadros & Fátima Menezes Bento

  2. Chemistry Institute, Department of Inorganic Chemistry, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9500, Porto Alegre, RS, Brazil

    Camila Correa, Marco Flôres Ferrão, Carolina Faganello Galeazzi & Tânia Mara Pizzolato

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  1. Gabriela Boelter
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  2. Juciana Clarice Cazarolli
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  3. Sabrina Anderson Beker
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  4. Patrícia Dörr de Quadros
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  8. Tânia Mara Pizzolato
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  9. Fátima Menezes Bento
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Correspondence to Gabriela Boelter.

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Boelter, G., Cazarolli, J.C., Beker, S.A. et al. Pseudallescheria boydii and Meyerozyma guilliermondii: behavior of deteriogenic fungi during simulated storage of diesel, biodiesel, and B10 blend in Brazil. Environ Sci Pollut Res 25, 30410–30424 (2018). https://doi.org/10.1007/s11356-018-3015-x

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