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Isolation of limonoid compound (Hamisonine) from endophytic fungi Penicillium oxalicum LA-1 (KX622790) of Limonia acidissima L. for its larvicidal efficacy against LF vector, Culex quinquefasciatus (Diptera: Culicidae)

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Abstract

Upon screening for novel and potential biocompounds with larvicidal activities, we successfully isolated hamisonine (HMSN) a limonoid compound from endophytic fungi Penicillium oxalicum LA-1 of Limonia acidissima. The extracted compound structure was elucidated by spectral studies such as UV-vis spectroscopy, thin-layer chromatography, FTIR, LC-ESI-MS, 1H NMR, and 13C NMR upon comparing with the spectral data available in the literature. Further, the isolated HMSN was tested against III and IV instar Culex quinquefasciatus larvae. The outcome of this study clearly emphasize that the extracted compound HMSN possesses a stupendous larvicidal activity in a dose-dependent manner with the LC50 and LC90 values of 1.779 and 7.685 ppm against III instar larvae and 3.031 and 28.498 ppm against IV instar larvae of C. quinquefasciatus, respectively. Interestingly, the histological studies evidently showing the damage of peritrophic membrane and epithelial cells of testing mosquito larvae.

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Acknowledgement

The authors are thankful to University grants commission-Special Assistance Programme (UGCSAP), New Delhi, Govt. of India for financial assistance Ref: 3-27/2011 (SAP-11) Dt/March, 2011.

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Authors and Affiliations

  1. Department of Biotechnology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, 620 024, India

    Prabukumar Seetharaman, Sathishkumar Gnanasekar, Rajkuberan Chandrasekaran & Sivaramakrishnan Sivaperumal

  2. Department of Biotechnology (FASH), Karpagam Academy of Higher Education, Karpagam University, Coimbatorei, Tamil Nadu, 641021, India

    Rajkuberan Chandrasekaran

  3. Agrifood Biotechnology (Academic Body of Agricultural and Food Biotechnology) Institute of Agricultural Sciences, Universidad Autónoma del Estado de Hidalgo Tulancingo, 43600, Tulancingo, HGO, Mexico

    Gobinath Chandrakasan

  4. Department of Botany and Microbiology, College of Science, King Saud University, P.O. 2455, Riyadh, 11451, Saudi Arabia

    Asad Syed, Mohamed S. Hodhod & Fuad Ameen

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  1. Prabukumar Seetharaman
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  2. Sathishkumar Gnanasekar
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Correspondence to Sivaramakrishnan Sivaperumal.

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Seetharaman, P., Gnanasekar, S., Chandrasekaran, R. et al. Isolation of limonoid compound (Hamisonine) from endophytic fungi Penicillium oxalicum LA-1 (KX622790) of Limonia acidissima L. for its larvicidal efficacy against LF vector, Culex quinquefasciatus (Diptera: Culicidae). Environ Sci Pollut Res 24, 21272–21282 (2017). https://doi.org/10.1007/s11356-017-9770-2

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