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Impact of heavy metal contamination on oxidative stress of Eisenia andrei and bacterial community structure in Tunisian mine soil

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Abstract

The aims of this work were firstly to study the effect of heavy metal-polluted soils from Tunisian mine on earthworm biochemical biomarkers and on bacterial communities and therefore to analyze the interaction between earth worms and bacterial communities in these contaminated soils. For this purpose, we had introduced earthworm Eisenia andrei in six soils: one from mine spoils and five from agricultural soils, establishing a gradient of contamination. The response of worms to the presence of heavy metal was analyzed at the biochemical and transcriptional levels. In a second time, the impact of worm on bacterial community structure was investigated using automated ribosomal intergenic spacer analysis (ARISA) fingerprinting. An impact of heavy metal-contaminated soils on the oxidative status of E. andrei was observed, but this effect was dependent of the level of heavy metal contamination. Moreover, our results demonstrate that the introduction of earthworms E. andrei has an impact on bacterial community; however, the major change was observed in the less contaminated site. Furthermore, a significant correlation between earthworm oxidative status biomarkers and bacterial community structure was observed, mainly in the mine spoils. Therefore, we contribute to a better understanding of the relationships between epigenic earthworms and bacterial communities in heavy metal-contaminated soils.

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Acknowledgements

This work was supported by funds from the “Ministère de l’Enseignement Supérieur et de la Recherche Scientifique; UR04A6R05. Biochimie et Toxicologie Environnementale” and by the Mistral Project “Them SICMED”. This work was also supported by funds from the laboratory “AMPERE Ecole Centrale de Lyon.”

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Authors and Affiliations

  1. Laboratory of Biochemistry and Environmental Toxicology, ISA Chott-Mariem, 4042, Sousse, Tunisia

    Iteb Boughattas, Hamadi Boussetta & Mohamed Banni

  2. Regional Research Centre in Horticulture and Organic Agriculture, Chott-Mariem, 4042, Sousse, Tunisia

    Sabrine Hattab

  3. Department of Sciences and Technological Innovation (DiSIT), University of Piemonte Orientale “A. Avogadro”, V. le T. Michel 11, 15121, Alessandria, Italy

    Mohamed Banni

  4. Laboratoire AMPERE Ecole Centrale de Lyon, 36, avenue Guy de Collongue, 69134, Ecully Cedex, France

    Elisabeth Navarro

  5. IRD-UMR LSTM, Campus de Baillarguet, 34398, Montpellier, France

    Elisabeth Navarro

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  1. Iteb Boughattas
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  2. Sabrine Hattab
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Correspondence to Mohamed Banni.

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Boughattas, I., Hattab, S., Boussetta, H. et al. Impact of heavy metal contamination on oxidative stress of Eisenia andrei and bacterial community structure in Tunisian mine soil. Environ Sci Pollut Res 24, 18083–18095 (2017). https://doi.org/10.1007/s11356-017-9449-8

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