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What about the detoxification mechanisms underlying ozone sensitivity in Liriodendron tulipifera?

  • Ozone and plant life: the Italian state-of-the-art
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Abstract

Liriodendron tulipifera (known as the tulip tree) is a woody species that has been previously classified as sensitive to ozone (O3) in terms of visible leaf injuries and photosynthetic primary reactions. The objective of this work is to give a thorough description of the detoxification mechanisms that are at the basis of O3 sensitivity. Biochemical and molecular markers were used to characterize the response of 1-year-old saplings exposed to O3 (120 ppb, 5 h day−1, for 45 consecutive days) under controlled conditions. O3 effects resulted in a less efficient metabolism of Halliwell-Asada cycle as confirmed by the diminished capacity to convert the oxidized forms of ascorbate and glutathione in the reduced ones (AsA and GSH, respectively). The reduced activity of AsA and GSH regenerating enzymes indicates that de novo AsA biosynthesis occurred. This compound could be a cofactor of several plant-specific enzymes that are involved in the early part of the phenylpropanoid and flavonoid biosynthesis pathway, as confirmed by the significant rise of PAL activity (+75%). The induction of the defence-related secondary metabolites (in particular, rutin and caffeic acid were about threefold higher) and the concomitant increase in transcript levels of PAL and CHS genes (+120 and 30%, respectively) suggest that L. tulipifera utilized this route in order to partially counteract the O3-induced oxidative damage.

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Acknowledgements

We gratefully acknowledge Dr. Arianna Dani and Dr. Romina Papini for the practical help in the laboratory. Mr. Andrea Parrini supervised the fumigation facilities. Thanks are due to Dr. Rodolfo Bernardi for the support in the molecular analyses. This study was supported by MIUR, Rome, project PRIN 2010-2011 “Planning the green city in the global change era: urban tree functions and suitability for predicted future climates (TreeCity).”

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Authors and Affiliations

  1. Department of Agriculture, Food and Environment, University of Pisa, Via del Borghetto 80, 56124, Pisa, Italy

    Elisa Pellegrini, Alessandra Campanella, Lorenzo Cotrozzi, Mariagrazia Tonelli, Cristina Nali & Giacomo Lorenzini

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  1. Elisa Pellegrini
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  2. Alessandra Campanella
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  3. Lorenzo Cotrozzi
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  4. Mariagrazia Tonelli
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  5. Cristina Nali
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  6. Giacomo Lorenzini
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Correspondence to Cristina Nali.

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Responsible editor: Philippe Garrigues

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Pellegrini, E., Campanella, A., Cotrozzi, L. et al. What about the detoxification mechanisms underlying ozone sensitivity in Liriodendron tulipifera?. Environ Sci Pollut Res 25, 8148–8160 (2018). https://doi.org/10.1007/s11356-017-8818-7

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  • DOI: https://doi.org/10.1007/s11356-017-8818-7

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