Abstract
Some pollutants can be resistant to wastewater treatment, hence becoming a risk to aquatic and terrestrial biota even at the very low concentrations (ng L−1–μg L−1) they are commonly found at. Tertiary treatments are used for micropollutant removal but little is known about the ecotoxicity of the treated effluent. In this study, a municipal secondary effluent was treated by a solar photo-Fenton reactor at initial neutral pH in a raceway pond reactor, and ecotoxicity was evaluated before and after micropollutant removal. Thirty-nine micropollutants were identified in the secondary effluent, mainly pharmaceuticals, with a total concentration of ≈80 μg L−1. After treatment, 99 % microcontaminant degradation was reached. As for ecotoxicity reduction, the assayed organisms showed the following sensitivity levels: Tetrahymena thermophila > Daphnia magna > Lactuca sativa > Spirodela polyrhiza ≈ Vibrio fischeri. The initial effluent showed an inhibitory effect of 40 % for T. thermophila and 20 % for D. magna. After 20 min of photo-Fenton treatment, no toxic effect was observed for T. thermophila and toxicity dropped to 5 % for D. magna.
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Altmann J, Ruhl AS, Zietzschmann F, Jekel M (2014) Direct comparison of ozonation and adsorption onto powdered activated carbon for micropollutant removal in advanced wastewater treatment. Water Res 55:185–193
Carra I, Casas López JL, Santos-Juanes L, Malato S, Sánchez Pérez JA (2013) Iron dosage as a strategy to operate the photo-Fenton process at initial neutral pH. Chem Eng J 224:67–74
Carra I, Santos-Juanes L, Acién Fernández FG, Malato S, Sánchez Pérez JA (2014) New approach to solar photo-Fenton operation. Raceway ponds as tertiary treatment technology. J Hazard Mater 279:322–329
Carra I, Sirtori C, Ponce-Robles L, Sánchez Pérez JA, Malato S, Agüera A (2015) Degradation and monitoring of acetamiprid, thiabendazole and their transformation products in an agro-food industry effluent during solar photo-Fenton treatment in a raceway pond reactor. Chemosphere 130:73–81
Cleuvers M (2003) Aquatic ecotoxicity of pharmaceuticals including the assessment of combination effects. Toxicol Lett 142:185–194
Dantas RF, Contreras S, Sans C, Esplugas S (2008) Sulfamethoxazole abatement by means of ozonation. J Hazard Mater 150:790–794
Daphtoxkit FTM magna (1998) Crustacean toxicity screening test for freshwater, Standard operational procedure. MicroBioTests Inc, Gent
Decree 109/2015 of March 17th establishing the regulation of discharges into hydraulic public and maritimal-terrestrial domain in Andalusia. BOJA nº89 (in Spanish)
EMEA (2006) Guideline on the environmental risk assessment of medicinal products for human use. The European Agency for the Evaluation of Medicinal Products: Committee for Medicinal Products for Human Use, EMEA/CHMP/SWP/4447/00
European Commission (2002) Decision 2002/657/EC of 12 August 2002. Off J European Union L221:8–36
Fent K, Weston AA, Caminada D (2006) Ecotoxicology of human pharmaceuticals. Aquat Toxicol 76:122–159
Fernández-Ibáñez P, Sichel C, Polo-López M, Cara-García M, Tello J (2009) Photocatalytic disinfection of natural well water contaminated by Fusarium solani using TiO2 slurry in solar CPC photo-reactors. Catalysis Today 144:62–68
Gerhardt A, Ud-Daula A, Schrammgerhardt KW (2010) Tetrahymena spp. (Protista, Ciliophora) as test species in rapid multilevel ecotoxicity tests. Acta Protozool 49:271–280
Gómez MJ, Martínez Bueno MJ, Lacorte S, Fernández-Alba AR, Agüera A (2007) Pilot survey monitoring pharmaceuticals and related compounds in a sewage treatment plant located on the Mediterranean coast. Chemosphere 66:993–1002
ISO (1998) ISO11348-3 Water quality—Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (luminescent bacteria test). International Organization for Standardization (ISO), Geneva
Jiang HS, Li M, Chang FY, Li W, Yin LY (2012) Physiological analysis of silver nanoparticles and AgNO3 toxicity to Spirodela polyrhiza. Environ Toxicol Chem 31:1880–1886
Klamerth N, Malato S, Agüera A, Fernández-Alba A, Mailhot G (2012) Treatment of municipal wastewater treatment plant effluents with modified photo-Fenton as a tertiary treatment for the degradation of micro pollutants and disinfection. Environ Sci Technol 5:2885–2892
Klamerth N, Malato S, Agüera A, Fernández-Alba A (2013) Photo-Fenton and modified photo-Fenton at neutral pH for the treatment of emerging contaminants in wastewater treatment plant effluents: a comparison. Water Res 47:833–840
Kortenkamp A, Backhaus T, Faust M (2009) State of the art report on mixture toxicology. In: Report Commissioned by the European Commission, DG Environment, pp 22–25
Láng J, Köhidai L (2012) Effects of the aquatic contaminant human pharmaceuticals and their mixtures on the proliferation and migratory responses of the bioindicator freshwater ciliate Tetrahymena. Chemosphere 89:592–601
Larsson DGJ, Pedro C, Paxeus N (2007) Effluent from drug manufactures contains extremely high levels of pharmaceuticals. J Hazard Mater 148:751–755
Maselli BS, Luna LAV, Palmeira JO, Tavares KP, Barbosa S, Beijo LA, Umbuzeiro GA, Kummrow F (2015) Ecotoxicity of raw and treated effluents generated by a veterinary pharmaceutical company: a comparison of the sensitivities of different standardized tests. Ecotoxicology 24(4):795–804
Nogueira RFP, Oliveira MC, Paterlini WC (2005) Simple and fast spectrophotometric determination of H2O2 in photo-Fenton reactions using metavanadate. Talanta 66:86–91
OECD (2004) Daphnia sp. acute immobilization test. Guidelines for the testing of chemicals, Test guideline 202. OECD, Paris
Ortega-Gómez E, Esteban García B, Ballesteros Martín MM, Fernández Ibáñez P, Sánchez Pérez JA (2014) Inactivation of natural enteric bacteria in real municipal wastewater by solar photo-Fenton at neutral pH. Water Res 63:316–324
Pereira R, Antunes SC, Gonçalves AMM, Marques SM, Gonçalves F, Ferreira F, Freitas AC, Rocha-Santos TAP, Diniz MS, Castro L, Peres I, Duarte AC (2009) The effectiveness of a biological treatment with Rhizopus oryzae and of a photo-Fenton oxidation in the mitigation of toxicity of a bleached kraft pulp mill effluent. Water Res 43:2471–2480
Petrie B, Barden R, Kasprzyk-Hordern B (2015) A review on emerging contaminants in wastewaters and the environment: current knowledge, understudied areas and recommendations for future monitoring. Water Res 72:3–27
Prieto-Rodríguez L, Oller I, Klamerth N, Agüera A, Rodríguez EM, Malato S (2013) Application of solar AOPs and ozonation for elimination of micropollutants in municipal wastewater treatment plant effluents. Water Res 47:1521–1528
Protoxkit F (1998) Freshwater toxicity test with a ciliate protozoan, Standard operational procedure. MicroBioTests Inc., Gent
RD 1620/2007, Royal Decree 1620/2007, on the legal system governing the reuse of treated water. BOE nº 294 (in Spanish)
Rivas G, Carra I, García Sánchez JL, Casas López JL, Malato S, Sánchez Pérez JA (2015) Modelling of the operation of raceway pond reactors for micropollutant removal by solar photo-Fenton as a function of photon absorption. Appl Catal B:-Environ 178:210–217
Rizzo L (2011) Bioassays as a tool for evaluating advanced oxidation processes in water and wastewater treatment. Water Res 45:4311–4340
Rizzo L, Meric S, Guida M, Kassinos D, Belgiorno V (2009) Heterogenous photocatalytic degradation kinetics and detoxification of an urban wastewater treatment plant effluent contaminated with pharmaceuticals. Water Res 43:4070–4078
Rodríguez-Chueca J, Polo-López MI, Mosteo R, Ormad MP, Fernández-Ibáñez P (2014) Disinfection of real and simulated urban wastewater effluents using a mild solar photo-Fenton. Appl Catal B:-Environ 150–151:619–629
Rodríguez-Gil JL, Catalá M, Alonso SG, Maroto RR, Valcárcel Y, Segura Y, Molina R, Melero JA, Martínez F (2010) Heterogeneous photo-Fenton treatment for the reduction of pharmaceutical contamination in Madrid rivers and ecotoxicological evaluation by a miniaturized fern spores bioassay. Chemosphere 80(4):381–388
Santos LHMLM, Araújo AN, Fachini A, Pena A, Delerue-Matos C, Montenegro MCBSM (2010) Ecotoxicological aspects related to the presence of pharmaceuticals in the aquatic environment. J Hazard Mater 175:45–95
Schaider LA, Rudel RA, Ackerman JM, Dunagan SC, Brody JG (2014) Pharmaceuticals, perfluorosurfactants, and other organic wastewater compounds in public drinking water wells in a shallow sand and gravel aquifer. Sci Total Environ 468–469:384–393
Schultz TW, Netzeva TI, Cronin MTD (2003) Selection of data sets for QSARs: analysis of Tetrahymena toxicity from aromatic compounds. Environ Res 14:59–81
SETAC. Society of Environmental Toxicology and Chemistry (2004) Whole effluent toxicity testing. SETAC, Pensacola, Available from: http://www.setac.org/wettre.html. Accessed 25 Mar 2016
Sobrero MC, Ronco A (2004) Acute toxicity test with lettuce seeds (Lactuca sativa L.). In: Castillo G (ed) Toxicological testing and methods for quality of water evaluation. Ottawa, pp 71–79 (in Spanish)
Spirodela duckweed Toxkit, 1998. Growth inhibition microbiotest with Spirodela polyrhiza. Standard Operational Procedure. Gent, Belgium
Tothill IE, Turner APF (1996) Developments in bioassay methods for toxicity testing in water treatment. TRAC – Trend Anal Chem 15:178–188
US EPA (1989) Protocols for short term toxicity screening of hazardous waste sites. A.8.7, Lettuce root elongation (Lactuca sativa). EUA, Chicago
US EPA (1991) Technical support document for water quality-based toxics control, EPA/505/2–90-001. Office of Water, EUA, Washington
Young BJ, Riera NI, Beily ME, Bres PA, Crespo DC, Ronco AE (2012) Toxicity of the effluent from an anaerobic bioreactor treating cereal residues on Lactuca sativa. Ecotox Environ Safe 76:182–186
Zhao D, Zhu C, Sun S, Yu H, Zhang L, Pan W, Zhang X, Yu H, Gu J, Cheng S (2007) Toxicity of pharmaceutical wastewater on male reproductive system of Mus musculus. Toxicol Ind Health 23:47–54
Acknowledgments
This research was supported by the Ministry for Economy and Competitiveness (Spanish Government) CTQ2013-46398-R, the Junta de Andalucía (Andalusian Regional Government) P10-RNM-05951, and the European Regional Development Fund (ERDF). Gracia Rivas Ibáñez would like to acknowledge the Andalusian Regional Government for her FPI scholarship.
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Freitas, A.M., Rivas, G., Campos-Mañas, M.C. et al. Ecotoxicity evaluation of a WWTP effluent treated by solar photo-Fenton at neutral pH in a raceway pond reactor. Environ Sci Pollut Res 24, 1093–1104 (2017). https://doi.org/10.1007/s11356-016-7101-7
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DOI: https://doi.org/10.1007/s11356-016-7101-7