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Lead removal in rats using calcium alginate

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Abstract

Lead (Pb) exposure, even at low levels, causes a variety of health problems. The aims of this study were to investigate the tissue distribution of lead in the bodies of rats, to evaluate lead removal from the internal organs and bones using calcium alginate in doses of 500, 200 and 100 mg/kg per day for 28 days and to assess the impact of calcium alginate on the level of essential elements. Lead (Pb), calcium (Ca), manganese (Mn), iron (Fe), copper (Cu) and zinc (Zn) levels in the blood, hearts, kidneys, livers and femurs of the experimental animals were measured using mass spectrometry with inductively coupled plasma. The results revealed that lead acetate exposure increased the levels of Pb in the blood and organs of the animals and significantly reduced contents of Ca, Mn, Fe, Cu and Zn. Treatment with calcium alginate in dose 500 mg/kg contributed to significant decreases in the amount of lead in the kidney, heart and bones of animals and a slight increase in the content of essential elements in the liver, kidneys and heart, although these changes were not significant. Decreasing of lead was not significant in the internal organs, bones and blood of animals treated with calcium alginate 200 and 100 mg/kg. Consequently, calcium alginate dose of 500 mg/kg more efficiently removes lead accumulated in the body. Calcium alginate does not have negative effect on level of essential elements quite the contrary; reducing the levels of lead, calcium alginate helps normalize imbalances of Ca, Mn, Fe, Cu and Zn. The results of this study suggest that calcium alginate may potentially be useful for the treatment and prevention of heavy metal intoxications.

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References

  • Abdulla M, Chmielnicka J (1990) New aspects on the distribution and metabolism of essential trace elements after dietary exposure to toxic metals. Biol Trace Elem Res 23:25–53

    Article  CAS  Google Scholar 

  • Adeyemi O, Ajayi JO, Olajuyin AM, Oloyede OB, Oladiji AT et al (2009) Toxicological evaluation of the effect of water contaminated with lead, phenol and benzene on liver, kidney and colon of albino rats. Food Chem Toxicol 47:885–887

    Article  CAS  Google Scholar 

  • Babalola OO, Okonji RE, Atoyebi JO, Sennuga TF, Raimi MM (2010) Distribution of lead in selected organs and tissues of albino rats exposed to acute lead toxicity. Sci Res Essay 5(9):845–848

    Google Scholar 

  • Bellinger DC (2011) The protean toxicities of lead: new chapters in a familiar story. Int J Environ Res Public Health 8(7):2593–2628

    Article  CAS  Google Scholar 

  • Blumenkrantz S, Asboe-Haunsen G (1973) New method for quantitative determination of uronic acids. Anal Biochem 54:484–489

    Article  CAS  Google Scholar 

  • Childhood lead poisoning (2010) World Health Organization, Geneva, Switzerland

  • Davis TA, Volesky B, Mucci A (2003) A review of the biochemistry of heavy metal biosorption by brown algae. Water Res 37:4311–4330

    Article  CAS  Google Scholar 

  • Eliaz I, Hotchkiss AT, Fihman ML, Rode D (2006) The effect of modified citrus pectin on urinary excretion of toxic elements. Phytother Res 20(10):859–864

    Article  CAS  Google Scholar 

  • Eliaz I, Weil E, Wilk B (2007) Integrative medicine and the role of modified citrus pectin/alginates in heavy metal chelation and detoxification—five case reports. Forsch Komplementärmed 14(6):358–364

    Article  Google Scholar 

  • Environmental Health Criteria 234: elemental speciation in human health risk assessment. (2006) World Health Organization, Geneva

  • Ercal N, Gurer-Orhan H, Aykin-Burns N (2001) Toxic metals and oxidative stress part 1: mechanisms involved in metal-induced oxidative damage. Curr Top Med Chem 1(6):529–539

    Article  CAS  Google Scholar 

  • Evaluation of certain food additives and naturally occurring toxicants (Thirty-ninth report of the joint FAO / WHO expert committee on food additives). (1993) World Health Organization Technical Report Series, No. 837

  • Flora SJS, Pachauri V (2010) Chelation in metal intoxication. Int J Environ Res Public Health 7(7):2745–2788

    Article  CAS  Google Scholar 

  • Goyer RA (1997) Toxic and essential metal interactions. Annu Rev Nutr 17:37–50

    Article  CAS  Google Scholar 

  • Gurer H, Ercal N (2000) Can antioxidants be beneficial in the treatment of lead poisoning? Free Radic Biol Med 29(10):927–945

    Article  CAS  Google Scholar 

  • Haug A (1961) The affinity of some divalent metals to different types of alginates. Acta Chem Scand 15:1794–1795

    Article  CAS  Google Scholar 

  • Haug A (1964) Composition and properties of alginates. In Report No. 30 Norwegian Inst. Of Seaweed Res, Trondheim, Norway

  • Haug A, Larsen B, Smidsrod O (1974) Uronic acid sequence in alginate from different sources. Carbonhydrate Res 32:217–225

    Article  CAS  Google Scholar 

  • Hodgkinson A, Nordin BE, Hambleton J, Oxby CB (1967) Radiostrontium absorption in man: suppression by calcium and by sodium alginate. Can Med Assoc J 97(19):1139–1143

    CAS  Google Scholar 

  • Jakubowski M (2011) Low-level environmental lead exposure and intellectual impairment in children- the current concept of risk assessment. Int J Occup Med Environ Health 24(1):1–7

    Article  Google Scholar 

  • Jomova K, Valko M (2011) Advances in metal-induced oxidative stress and human disease. Toxicology 283(2–3):65–87

    Article  CAS  Google Scholar 

  • Khotimchenko M, Serguschenko I, Khotimchenko Y (2006) Lead absorption and excretion in rats given insoluble salts of pectin and alginate. Int J Toxicol 25(3):195–203

    Article  CAS  Google Scholar 

  • Khotimchenko M, Poleschuk T, Savchenko O, Shokur O (2013) Comparative lead-removing activity of the non-starch polysaccharides. J Med Sci 13(8):647–656

    Article  Google Scholar 

  • Kravtchenko TP, Pilnik A (1990) A simplified method for the determination of the intrinsic viscosity of pectin solutions by classical viscosimetry. In: Phillips CO, Williams PA (eds) Gums and stabilizers in the food industry, vol 5. IRL Press, Oxford, pp 281–285

    Google Scholar 

  • Lanphear BP, Hornung R, Khoury J et al (2005) Low-level environmental lead exposure and children’s intellectual function: an international pooled analysis. Environ Health Perspect 113(7):894–899

    Article  CAS  Google Scholar 

  • Lead Exposure in Children Affects Brain and Behavior. (2012) Facts for families No. 45

  • Lead exposure in children: prevention, detection, and management. American Academy of Pediatrics Committee on Environmental Health (2005) Pediatrics 116(4):1036–1046

  • Lidsky TI, Schneider JS (2003) Lead neurotoxicity in children: basic mechanisms and clinical correlates. Brain 126(1):5–19

    Article  Google Scholar 

  • Low Level Lead Exposure Harms Children: A Renewed Call for Primary Prevention. (2012) Report of the advisory committee on childhood lead poisoning prevention of the Centers for Disease Control and Prevention

  • Mortada WI, Sobh MA, El-Defrawy MM, Farahat SE (2001) Study of lead exposure from automobile exhaust as a risk for nephrotoxicity among traffic policemen. Am J Nephrol 21(4):274–279

    Article  CAS  Google Scholar 

  • Naja GM, Volesky B, Murphy V (2010) Biosorption, metals. In: Flickinger M (ed) Encyclopedia of Industrial Biotechnology. Wiley Interscience, New York, pp 1–29

    Google Scholar 

  • Patrick L (2006) Lead toxicity part II: the role of free radical damage and the use of antioxidants in the pathology and treatment of lead toxicity. Altern Med Rev 11(2):114–127

    Google Scholar 

  • Petering HG (1978) Some observation on the interaction of zinc, copper, and iron metabolism in lead and cadmium toxicity. Environ Health Perspect 25:141–145

    Article  CAS  Google Scholar 

  • Protecting Children from Harmful Chemical Exposures Chemical Safety and Children’s Health. (2003) Intergovernmental forum on chemical safety. Fourth session – FORUM IV. IFCS FSC Working Group Chaired by Hungary

  • Schiewer S, Volesky B (2000) Biosorption by marine algae. In: Valdes JJ (ed) Remediation. Kluwer Academic Publishers, Dodrecht, the Netherlands, pp 139–169

  • Smith DR, Calacsan LC, Woolard D, Luck M, Cremin J et al (2000) Succimer and the urinary excretion of essential elements in a primate model of childhood lead exposure. Toxicol Sci 54:473–480

    Article  CAS  Google Scholar 

  • Sutton A, Harrison GE, Carr TE, Barltop D (1971) Reduction in absorption of dietary strontium in children by an alginate derivative. Br J Radiol 44(523):567

    CAS  Google Scholar 

  • Sutton A, Shepherd H (1973) Urinary Barium excretion in man and its reduction by alginate. Health Phys 25(2):182–184

    CAS  Google Scholar 

  • Timchalk C, Lin Y, Weitz KK, Wu H, Gies RA et al (2006) Disposition of lead (Pb) in saliva and blood of Sprague-Dawley rats following a single or repeated oral exposure to Pb-acetate. Toxicology 222:86–94

    Article  CAS  Google Scholar 

  • Torres-Alanis O, Garza-Ocañas L, Bernal MA, Piñeyro-Lopez A (2000) Urinary excretion of trace elements in humans after sodium 2,3-dimercaptopropane-1-sulfonate challenge test. J Toxicol Clin Toxicol 38(7):697–700

    Article  CAS  Google Scholar 

  • Toxicological profile for lead. (2007) U.S. Department of Health and Human Services. Public Health Service. Agency for Toxic Substances and Disease Registry

  • Van Der Borgth O, Colard J, Boulenger R (1972) Human fecal Ra-226 excretion shortly after a RaSO4 dust contamination, with alginate treatment. Health Phys 23:240–243

    Google Scholar 

  • Victery W, Thomas D, Shoeps P, Vander AJ (1982) Lead increases urinary zinc excretion in rats. Biol Tr Elem Res 4:211–19

    Article  CAS  Google Scholar 

  • Washington State Lead Chemical Action Plan. (2009) Department of Ecology State of Washington. Washington State Department of Health. WA

  • Zhao ZY, Liang L, Fan X, Yu Z, Hotchkiss AT et al (2008) The role of modified citrus pectin as an effective chelator of lead in children hospitalized with toxic lead levels. Altern Ther Health Med 14(4):34–38

    Google Scholar 

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Authors and Affiliations

  1. A.V. Zhirmunsky Institute of Marine Biology, Far Eastern Branch of Russian Academy of Sciences, 17 Palchevskogo St., Vladivostok, 690041, Russia

    Olga V. Savchenko & Marina N. Sgrebneva

  2. Far Eastern Geological Institute, Far Eastern Branch of the Russian Academy of Sciences, 159 Prospekt 100-letiya, Vladivostok, 690022, Russia

    Vladimir I. Kiselev

  3. School of Biomediсine, Far Eastern Federal University, 8 Suhanova St., Vladivostok, 690950, Russia

    Yuri S. Khotimchenko

Authors
  1. Olga V. Savchenko
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  2. Marina N. Sgrebneva
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  3. Vladimir I. Kiselev
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  4. Yuri S. Khotimchenko
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Correspondence to Olga V. Savchenko.

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Responsible editor: Philippe Garrigues

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Savchenko, O.V., Sgrebneva, M.N., Kiselev, V.I. et al. Lead removal in rats using calcium alginate. Environ Sci Pollut Res 22, 293–304 (2015). https://doi.org/10.1007/s11356-014-3324-7

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  • DOI: https://doi.org/10.1007/s11356-014-3324-7

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