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Oxidative stress status, antioxidant metabolism and polypeptide patterns in Juncus maritimus shoots exhibiting differential mercury burdens in Ria de Aveiro coastal lagoon (Portugal)

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Abstract

This study assessed the oxidative stress status, antioxidant metabolism and polypeptide patterns in salt marsh macrophyte Juncus maritimus shoots exhibiting differential mercury burdens in Ria de Aveiro coastal lagoon at reference and the sites with highest, moderate and the lowest mercury contamination. In order to achieve these goals, shoot-mercury burden and the responses of representative oxidative stress indices, and the components of both non-glutathione- and glutathione-based H2O2-metabolizing systems were analyzed and cross-talked with shoot-polypeptide patterns. Compared to the reference site, significant elevations in J. maritimus shoot mercury and the oxidative stress indices such as H2O2, lipid peroxidation, electrolyte leakage and reactive carbonyls were maximum at the site with highest followed by moderate and the lowest mercury contamination. Significantly elevated activity of non-glutathione-based H2O2-metabolizing enzymes such as ascorbate peroxidase and catalase accompanied the studied damage-endpoint responses, whereas the activity of glutathione-based H2O2-scavenging enzymes glutathione peroxidase and glutathione sulfo-transferase was inhibited. Concomitantly, significantly enhanced glutathione reductase activity and the contents of both reduced and oxidized glutathione were perceptible in high mercury-exhibiting shoots. It is inferred that high mercury-accrued elevations in oxidative stress indices were obvious, where non-glutathione-based H2O2-decomposing enzyme system was dominant over the glutathione-based H2O2-scavenging enzyme system. In particular, the glutathione-based H2O2-scavenging system failed to coordinate with elevated glutathione reductase which in turn resulted into increased pool of oxidized glutathione and the ratio of oxidized glutathione-to-reduced glutathione. The substantiation of the studied oxidative stress indices and antioxidant metabolism with approximately 53-kDa polypeptide warrants further studies.

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Acknowledgments

Authors gratefully acknowledge the financial supports received from both FCT (Government of Portugal) through contract (SFRH/BPD/64690/2009; SFRH/BPD/84671/2012) and by the Aveiro University Research Institute/CESAM.

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Authors and Affiliations

  1. Department of Chemistry and CESAM-Centre for Environmental and Marine Studies, University of Aveiro, 3810-193, Aveiro, Portugal

    Naser A. Anjum, Armando C. Duarte, Eduarda Pereira & Iqbal Ahmad

  2. Department of Biology and CESAM-Centre for Environmental and Marine Studies, University of Aveiro, 3810-193, Aveiro, Portugal

    Iqbal Ahmad

Authors
  1. Naser A. Anjum
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  2. Armando C. Duarte
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  3. Eduarda Pereira
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  4. Iqbal Ahmad
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Correspondence to Iqbal Ahmad.

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Responsible editor: Elena Maestri

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Anjum, N.A., Duarte, A.C., Pereira, E. et al. Oxidative stress status, antioxidant metabolism and polypeptide patterns in Juncus maritimus shoots exhibiting differential mercury burdens in Ria de Aveiro coastal lagoon (Portugal). Environ Sci Pollut Res 21, 6652–6661 (2014). https://doi.org/10.1007/s11356-014-2578-4

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  • DOI: https://doi.org/10.1007/s11356-014-2578-4

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