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DNA integrity of onion root cells under catechol influence

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Abstract

Catechol is a highly toxic organic pollutant, usually abundant in the waste effluents of industrial processes and agricultural activities. The environmental sources of catechol include pesticides, wood preservatives, tanning lotion, cosmetic creams, dyes, and synthetic intermediates. Genotoxicity of catechol at a concentration range 5 × 10−1–5 mM was evaluated by applying random amplified polymorphic DNA (RAPD) and time-lapse DNA laddering tests using onion (Allium cepa) root cells as the assay system. RAPD analysis revealed polymorphisms in the nucleotidic sequence of DNA that reflected the genotoxic potential of catechol to provoke point mutations, or deletions, or chromosomal rearrangements. Time-lapse DNA laddering test provided evidence that catechol provoked DNA necrosis and apoptosis. Acridine orange/ethidium bromide staining could distinguish apoptotic from necrotic cells in root cells of A. cepa.

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Authors and Affiliations

  1. Fruit Trees Research Unit, C.R.A., Via Torrino 3, 81100, Caserta, Italy

    Milena Petriccione

  2. Department of Life Science, Second University of Naples, Via Vivaldi 43, 81100, Caserta, Italy

    Valentina Forte, Diego Valente & Claudia Ciniglia

Authors
  1. Milena Petriccione
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  2. Valentina Forte
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  3. Diego Valente
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  4. Claudia Ciniglia
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Correspondence to Claudia Ciniglia.

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Responsible editor: Philippe Garrigues

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Petriccione, M., Forte, V., Valente, D. et al. DNA integrity of onion root cells under catechol influence. Environ Sci Pollut Res 20, 4859–4871 (2013). https://doi.org/10.1007/s11356-012-1422-y

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  • DOI: https://doi.org/10.1007/s11356-012-1422-y

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