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Effects of vibratory platform training on the histomorphometric parameters of the soleus muscle in obese Wistar rats

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Abstract

Background

The increasing of obesity is one of the main challenges of public health, with an incentive to the development of healthy life habits and practise of physical exercise. Among the physical exercises, the use of whole body vibration is highlighted, which can promote gain of muscle strength.

Aims

This study aimed atevaluating the effects that vibratory platform causes on the histomophometry and fiber types of the soleus muscle of Wistar rats with induced obesity by monosodium glutamate (MSG).

Methods

Thirty-two male Wistar rats were used, 16 of which were induced by obesity with intradermal injections of MSG, equally randomized into four groups: control (GC), control with intervention (GCP), obese (GO), and obese with intervention (GOP). At the 70 days, the training on vibratory platform was started adapted to a frequency of 60 Hz and amplitude of 2 mm, performed 3 times a week, with a duration of 10 min, during 8 consecutive weeks. At 130 days, the animals were weighed and the nasoanal length was measured; then they were euthanized and the soleus muscles were collected and processed for analysis. Data were statistically analyzed about the homogeneity of variances by the Bartlett’s test, about the normality by Shapiro–Wilk’s test, then ANOVA of two factors and Tukey-HSD follow-up tests, adopting the significance level of 5%.

Results

Morphometrically, the obese groups presented muscle hypotrophy (p < 0.01) and the training caused increase in the fibers (p = 0.03), increase in the number of nuclei (p < 0.01), and decrease of connective tissue (p < 0.01). Meanwhile, there was no distinction among the muscle fiber types after the training on the vibratory platform, which is composed of oxidative fibers.

Conclusion

The training on the vibratory platform induced beneficial effects in the muscle tissue of obese rats; however, both obesity and training did not influence on the fiber type of the soleus muscle.

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Abbreviations

BW:

Body weight

CEUA:

Animal Ethics Committee

GC:

Control group

GCP:

Control group + vibratory platform

GO:

Obese group

GOP:

Obese group + vibratory platform

LABEF:

Laboratory of structural and functional biology

LELRF:

Laboratory of physical injury and physiotherapeutic resources

MSG:

Monosodium glutamate

NL:

Nasoanal length

NCDs:

Chronic non-communicable diseases

OS:

Obesity sarcopenic

UNIOESTE:

Universidade Estadual do Oeste do Paraná

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Authors and Affiliations

  1. Laboratórios de Estudo de Lesões e Recursos Fisioterapêuticos e de Biologia Estrutural e Funcional, Centro de Ciências Biológicas e da Saúde, Universidade Estadual Do Oeste Do Paraná (UNIOESTE), Campus Cascavel, Universitária St 2069, Cascavel, PR, 85819-110, Brazil

    Mariana Laís Boaretto, Bárbara Zanardini de Andrade, Jhyslayne Ignácia Hoff Nunes Maciel, Iranilda Moha, Márcia Miranda Torrejais, Lucinéia de Fátima Chasko Ribeiro & Gladson Ricardo Flor Bertolini

  2. Laboratório de Fisiologia Endócrina e Metabolismo, UNIOESTE, Campus Cascavel, Cascavel, Brazil

    Sara Cristina Sagae Schneider

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  1. Mariana Laís Boaretto
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Correspondence to Gladson Ricardo Flor Bertolini.

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The research Project was approved by the Animal Ethics Committee (CEUA) from UNIOESTE (Number 08/18).

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Boaretto, M.L., de Andrade, B.Z., Maciel, J.I.H.N. et al. Effects of vibratory platform training on the histomorphometric parameters of the soleus muscle in obese Wistar rats. Sport Sci Health 16, 501–510 (2020). https://doi.org/10.1007/s11332-020-00632-8

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