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In vivo Cell Tracking Using Non-invasive Imaging of Iron Oxide-Based Particles with Particular Relevance for Stem Cell-Based Treatments of Neurological and Cardiac Disease

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A Correction to this article was published on 15 June 2021

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Abstract

Stem cell-based therapeutics is a rapidly developing field associated with a number of clinical challenges. One such challenge lies in the implementation of methods to track stem cells and stem cell-derived cells in experimental animal models and in the living patient. Here, we provide an overview of cell tracking in the context of cardiac and neurological disease, focusing on the use of iron oxide-based particles (IOPs) visualized in vivo using magnetic resonance imaging (MRI). We discuss the types of IOPs available for such tracking, their advantages and limitations, approaches for labeling cells with IOPs, biological interactions and effects of IOPs at the molecular and cellular levels, and MRI-based and associated approaches for in vivo and histological visualization. We conclude with reviews of the literature on IOP-based cell tracking in cardiac and neurological disease, covering both preclinical and clinical studies.

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Acknowledgments

All of the authors are members of the COST action 16122, Biomaterials and advanced physical techniques for regenerative cardiology and neurology (BIONECA), which has supported seminars and workshops that have served as the springboard for composing this review.

Funding

JCG and JLB are supported by the Norwegian Research Council (grants 230000, 189374, 229654) and the Southeastern Norway Health Authority (grants 2011035, 2015045, 2015102, 2014119). MP and JL are supported by ARRS grants J7-7424, Z4-8229 and P1-0055. DM is supported by the Croatian Science Foundation (IP-2016-06-9451) and co-financed by the European Union through the European Regional Development Fund, Operational Programme Competitiveness and Cohesion, grant agreement no. KK.01.1.1.01.0007, CoRE – Neuro.

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  1. Markus Aswendt, Jean-Luc Boulland, Jasna Lojk and Stefan Stamenković contributed equally to this work.

Authors and Affiliations

  1. Laboratory for Neural Development and Optical Recording (NDEVOR), Department of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, PB 1105, Blindern, Oslo, Norway

    Joel C. Glover & Jean-Luc Boulland

  2. Norwegian Center for Stem Cell Research, Oslo University Hospital, Oslo, Norway

    Joel C. Glover & Jean-Luc Boulland

  3. Institut für Neurowissenschaften und Medizin, Forschungszentrum Jülich, Leo-Brandt-Str. 5, 52425, Jülich, Germany

    Markus Aswendt & Mathias Hoehn

  4. Group for Nano and Biotechnological Applications, Faculty of Electrical Engineering, University of Ljubljana, Trzaska cesta 25, Ljubljana, Slovenia

    Jasna Lojk & Mojca Pavlin

  5. Center for Laser Microscopy, Department of Physiology and Biochemistry, Faculty of Biology, University of Belgrade, PB 52, 10001 Belgrade, Serbia

    Stefan Stamenković & Pavle Andjus

  6. Department of Applied Physics, Università Politecnica delle Marche - Di.S.C.O., Via Brecce Bianche, 60131, Ancona, Italy

    Fabrizio Fiori

  7. Laboratory for Stem Cells, Croatian Institute for Brain Research, School of Medicine, University of Zagreb, Zagreb, Croatia

    Dinko Mitrecic

  8. Institute of Biophysics, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, Ljubljana, Slovenia

    Mojca Pavlin

  9. Department of Medicine and Surgery, University of Parma, Parma, Italy

    Stefano Cavalli, Caterina Frati & Federico Quaini

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Glover, J.C., Aswendt, M., Boulland, JL. et al. In vivo Cell Tracking Using Non-invasive Imaging of Iron Oxide-Based Particles with Particular Relevance for Stem Cell-Based Treatments of Neurological and Cardiac Disease. Mol Imaging Biol 22, 1469–1488 (2020). https://doi.org/10.1007/s11307-019-01440-4

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