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MS-based metabolite profiling reveals time-dependent skin biomarkers in UVB-irradiated mice

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Abstract

We observed clinical and histological changes, including increased transepidermal water loss, epidermal thickness, and inflammatory cells, and changes in collagen fibers in the skin of mice chronically exposed to ultraviolet (UV) B radiation for 12 weeks. By using ultra-performance liquid chromatography-quadrupole time-of-flight (TOF) mass spectrometry (MS), gas chromatography (TOF–MS), and NanoMate tandem-MS-based metabolite profiling, we identified amino acids, organic compounds, fatty acids, lipids, nucleosides, carbohydrates, lysophosphatidylcholines, lysophosphatidylethanolamines, urocanic acids, and ceramides (CERs) as candidate biomarkers of the histological changes in mouse skin following UVB irradiation for 6 and 12 weeks. cis-Urocanic acid and cholesterol showed the most dramatic increase and decrease at 6 and 12 weeks, respectively. In addition, the changes in skin primary metabolites and lysophospholipids induced by UVB exposure were generally greater at 12 weeks than at 6 weeks. The results from primary metabolite, lysophospholipid, and CER profiles suggest that prolonged chronic exposure to UVB light has a great effect on skin by altering numerous metabolites. A comprehensive MS-based metabolomic approach for determining regulatory metabolites in UV-induced skin will lead to a better understanding of the relationship between skin and UV.

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Acknowledgments

This study was supported by a grant from the Korea Healthcare Technology R&D Project (Grant No. A103017), Ministry of Health and Welfare, and by the Korea Basic Science Institute (Grant No. T33409).

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Authors and Affiliations

  1. Department of Bioscience and Biotechnology, Kon-Kuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 143-701, South Korea

    Hye Min Park & Choong Hwan Lee

  2. College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu, 702-701, South Korea

    Jung-Hoon Shin & Kwang-Hyeon Liu

  3. Food Research Institute, AmorePacific R&D Center, 1920 Yonggu-daero, Giheung-gu, Yongin, Gyeonggi-do, 446-729, South Korea

    Jeong Kee Kim & Sang Jun Lee

  4. Integrated Metabolomics Research Group of Seoul Center, Korea Basic Science Institute, Seoul, 136-701, South Korea

    Geum-Sook Hwang

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  1. Hye Min Park
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  2. Jung-Hoon Shin
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  3. Jeong Kee Kim
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Correspondence to Choong Hwan Lee.

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Park, H.M., Shin, JH., Kim, J.K. et al. MS-based metabolite profiling reveals time-dependent skin biomarkers in UVB-irradiated mice. Metabolomics 10, 663–676 (2014). https://doi.org/10.1007/s11306-013-0594-x

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