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Intraspecific discrimination study of wild cherry populations from North-Western Turkey by DNA barcoding approach

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Abstract

Wild cherry (Prunus avium L.) is a plant widely distributed around the world that possesses a great economic value. Indeed, it represents both a fruit source and one of the most important European hardwood species. For this reason, in order to obtain information about Turkish authoctonous P. avium germplasm and favor wood management policies and preservation strategies, we investigated the phylogenetic relationships existing among seven wild cherry populations (a total of 139 individuals) located in Northern Turkey using DNA barcoding. For each specimen, in detail, we sequenced a nuclear one (ITS) and two plastidial (trnH-psbA and matK) genes, to identify nucleotide polymorphisms, hypervariable genetic regions, and mutation events. Applying neighbor-joining method and genetic structure analysis, a high rate of crossbreeding among stands was revealed, except for one population (Gölcük) whose molecular profile was less similar to the others. In general, we observed that ITS was the most informative marker, suggesting it as a good candidate for P. avium intraspecific study. We conclude that DNA barcode technique, usually applied for species identification, may be also used as a scientific tool for the detection of plant biodiversity at population level.

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Acknowledgments

The authors want to thank Prof. Ahu Altınkut Uncuoğlu and Dr. Ezgi Çabuk Şahin for their great contribution in data analysis and Miss Sophie Gart who revised the English form of this manuscript.

Data archiving statement

Genetic data were registered in GenBank database. ID number of each deposited sequence, with relative details, was reported in Supplemental Material - Table S1 and copied below.

Population

trnH-psbA

matK

ITS

Abant Population (A)

MF431607

MF431608

MF431606

Molla Fenari Population (B)

MF590194

MF590188

MF590200

Yedigöller Population (C)

MF590195

MF590189

MF590201

Düzce Population (D)

MF590197

MF590190

MF590202

Melen Population (E)

MF590196

MF590191

MF590203

Kefken Population (F)

MF590198

MF590192

MF590204

Gölcük Population (G)

MF590199

MF590193

MF590205

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Authors and Affiliations

  1. Department of Molecular Biology and Genetics, Faculty of Basic Sciences, Gebze Technical University, 41400, Kocaeli, Turkey

    Selin Gül Ünsal, Yelda Özden Çiftçi & Burcu Uzan Eken

  2. Department of Biology, University of Rome “Tor Vergata”, via della Ricerca Scientifica 1, 00133, Rome, Italy

    Burcu Uzan Eken & Ercan Velioğlu

  3. Republic of Turkey General Directorate of Forestry, Poplar and Rapidly Developing Forest Trees Research Institute, 41140, Kocaeli, Turkey

    Selin Gül Ünsal, Gabriele Di Marco, Angelo Gismondi & Antonella Canini

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  1. Selin Gül Ünsal
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Contributions

AG, AC YOC, and EV designed research; SGU performed research; BUK carried out the sampling; EV authorized the sampling; SGU, AG, and GDM analyzed data; SGU and AG wrote the paper; AC provided financial support; all authors edited, revised, and provided comments to the manuscript.

Corresponding author

Correspondence to Angelo Gismondi.

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The authors declare that they have no conflict of interest.

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Communicated by E. Dirlewanger

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Key message

Application of DNA barcoding at intraspecific level, to investigate phylogenetic relationships existing among Turkish wild cherry populations, increase genetic knowledge of this species, and promote its conservation and breeding programs

Angelo Gismondi and Antonella Canini are co-last authors of this manuscript.

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Ünsal, S.G., Çiftçi, Y.Ö., Eken, B.U. et al. Intraspecific discrimination study of wild cherry populations from North-Western Turkey by DNA barcoding approach. Tree Genetics & Genomes 15, 16 (2019). https://doi.org/10.1007/s11295-019-1323-z

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  • DOI: https://doi.org/10.1007/s11295-019-1323-z

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