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SSR-based genetic mapping and QTL analysis for timing of spring bud flush, young shoot color, and mature leaf size in tea plant (Camellia sinensis)

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Abstract

Tea plant (Camellia sinensis) is a major beverage crop across the world. To uncover the genetic controls of agronomic traits and facilitate marker-assisted breeding (MAB) in tea plant, we constructed a saturated SSR-based linkage map using an F1 population derived from the crossing of ‘Longjin43’ × ‘Baihaozao’. A total of 483 SSR markers, consisting of 117 novel loci, 129 transferred from other tea plant maps, and 237 previously mapped, were successfully integrated into a new consensus map. The map has 15 linkage groups, covering 1226.2 cM in total with an average marker distance of 2.5 cM. The 126 markers in common enabled us to align this map to the reference genetic maps of tea plant. Phenotype data were collected in 2014 and 2015 for five traits: timing of spring bud flush (TBF), young shoot color (YSC), mature leaf length (MLL), mature leaf width (MLW), and leaf shape index (LSI, i.e., MLL/MLW). QTL analyses were performed for the five traits using the new consensus map and 15 QTLs were identified. The SSR markers, linkage map, and QTLs reported here are useful resources for future QTL mining, identification of causal genes, and MAB in tea plant.

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Acknowledgments

We would like to give special thanks to Prof. Shunyuan Xiao from the University of Maryland and the anonymous reviewers for their constructive comments on the manuscript. This work was supported by The Earmarked Fund for Modern Agro-industry Technology Research System (nycytx-23) and the Twelfth Five-Year Project of Sichuan Province for Tea Plant Breeding, Science and Technology Department Project of Sichuan Province (2012-12CGZHZX0579).

Data archiving statement

We followed standard Tree Genetics and Genomes policy. The primer sequences and marker positions on the consensus map are provided in Online Resource 1. The genotype and phenotype data are provided in Online Resources 2 and 6, respectively.

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    Authors and Affiliations

    1. National Center for Tea Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences, 9 Meiling South Road, Hangzhou, Zhejiang, 310008, China

      Li-Qiang Tan, Li-Yuan Wang, Li-Yi Xu, Li-Yun Wu, Cheng-Cai Zhang, Kang Wei, Pei-Xian Bai, Hai-Lin Li & Hao Cheng

    2. College of Horticulture, Sichuan Agricultural University, 211 Huimin Road, Chengdu, Sichuan, 611130, China

      Li-Qiang Tan, Li-Yi Xu, Min Peng & Gui-Nian Qi

    3. Key Laboratory of Tea Plant Biology and Resources Utilization, Ministry of Agriculture, 9 Meiling South Road, Hangzhou, Zhejiang, 310008, China

      Li-Yuan Wang, Li-Yun Wu, Cheng-Cai Zhang, Kang Wei, Pei-Xian Bai, Hai-Lin Li & Hao Cheng

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    1. Li-Qiang Tan
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    2. Li-Yuan Wang
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    3. Li-Yi Xu
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    Correspondence to Hao Cheng or Gui-Nian Qi.

    Additional information

    Communicated by Z. Kaya

    Li-Qiang Tan and Li-Yuan Wang contributed equally to this work.

    Electronic supplementary material

    Below is the link to the electronic supplementary material.

    Online Resource 1

    Positions and primer sequences of the SSR markers on the consensus map. (PDF 60.3 kb)

    Online Resource 2

    (XLSX 365 kb)

    Online Resource 3

    (JPG 714 kb)

    High Resolution Image (TIF 6.39 kb)

    Online Resource 4

    (JPG 381 kb)

    High Resolution Image (TIF 6 kb)

    Online Resource 5

    Allelic and zygotic segregation distortion test results in the ‘L43’ × ‘BHZ’ population. (XLSX 13.6 kb)

    Online Resource 6

    (XLSX 34.5 kb)

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    Tan, LQ., Wang, LY., Xu, LY. et al. SSR-based genetic mapping and QTL analysis for timing of spring bud flush, young shoot color, and mature leaf size in tea plant (Camellia sinensis). Tree Genetics & Genomes 12, 52 (2016). https://doi.org/10.1007/s11295-016-1008-9

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