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Analysis of the molecular variation between and within cultivated and wild Pistacia species using AFLPs

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Abstract

Knowledge of pistachio genetic diversity is necessary for the formulation of appropriate management strategies for the conservation of these species. We analysed amplified fragment length polymorphisms in a total of 216 pistachio accessions, which included seven populations from three wild species (Pistacia vera, Pistacia khinjuk and Pistacia atlantica subsp. kurdica) and most of the important cultivars from Iran, together with some foreign cultivars. High levels of genetic diversity were detected within the Iranian cultivars, and they showed a clear separation from foreign cultivars, as revealed by unweighted pair group method with arithmetic averaging and supported by analysis of molecular variance. The lowest amount of polymorphism was observed in P. atlantica subsp. kurdica, which showed the lowest number of total bands as compared to the other species. This revealed strong genetic erosion of P. atlantica subsp. kurdica, which reflected a severe decline in habitat and over-exploitation. Based on these findings, strategies are proposed for the genetic conservation and management of pistachio species and cultivars.

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Acknowledgments

This study was supported by the Agricultural Biotechnology Research Institute of Iran. The authors would like to thank Dr. Hasan Maddah Arefy from the Research Institute of Forests and Rangelands of Iran for the photographs of pistachio. They would also like to thank Dr A. A. Javanshah (Director General of the Iranian Pistachio Research Institute), in particular, for his contribution to the collection of plant material.

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Authors and Affiliations

  1. Department of Genomics, Agricultural Biotechnology Research Institute of Iran, Mahdasht Road, Karaj, Iran

    Parvin Salehi Shanjani, Mohsen Mardi, Leila Pazouki, S. Mostafa Pirseyedi, Mohammad Reza Ghaffari & S. Mojtaba Khayam Nekoui

  2. Natural Resources Gene Bank, Research Institute of Forests and Rangelands, P.O. Box 13185-116, Tehran, Iran

    Parvin Salehi Shanjani

  3. Pomology Laboratory, Department of Crop Science, Agricultural University of Athens, Athens, Greece

    Marianna Hagidimitriou

  4. CRA–Istituto Sperimentale per la Frutticoltura di Roma, via di Fioranello 52, 00134, Rome, Italy

    Damiano Avanzato

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  1. Parvin Salehi Shanjani
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Correspondence to Mohsen Mardi.

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Shanjani, P.S., Mardi, M., Pazouki, L. et al. Analysis of the molecular variation between and within cultivated and wild Pistacia species using AFLPs. Tree Genetics & Genomes 5, 447–458 (2009). https://doi.org/10.1007/s11295-008-0198-1

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  • DOI: https://doi.org/10.1007/s11295-008-0198-1

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