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Gut microbiota regulation and prebiotic properties of polysaccharides from Oudemansiella raphanipes mushroom

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A Correction to this article was published on 29 July 2023

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Abstract

Oudemansiella raphanipes is a type of fungus used as both medicine and food. Fungal polysaccharides have demonstrated various bioactivities, involving the adjust and control of gut microbiota, but no such studies on O. raphanipes polysaccharides (OrPs) have been reported. It is by extracting and purifying that OrPs was obtained from O. raphanipes crude polysaccharide and study their effects in mice. The sample contents of total sugar was 97.26%, and the monosaccharide content comprised mannose, rhamnose, glucose, and xylose in a molar ratio of 35.2:2.8:21.2:40.8. The effects of OrPs on body weight (BW), gut microbiota, fecal short-chain fatty acids (SCFAs), and the correlation between fecal SCFAs and gut microbes, in mice were investigated. The results of the experiment found that OrPs significantly (P < 0.01) inhibited the increase in BW, altered the constitution of the gut microbiota, and significantly (P < 0.05) enhanced the content of fecal SCFAs in mice. Moreover, among the top ten bacteria in terms of relative abundance, the Lachnospiraceae and Lachnospiraceae NK4A136 groups were positively associated with the increased production of SCFAs. Other bacteria, such as Atopobiaceae and Bifidobacterium of Actinobacteriota, and Faecalibaculum, Dubosiella, and Clostridium sensu stricto 5 of Firmicutes, were also positively associated with higher content of fecal SCFAs. The results of the experiment suggest that OrPs have a potential prebiotic effect on gut microbiota and may prevent BW gain. Furthermore, the major producers of SCFAs were Firmicutes and Actinobacteriota.

Key Points

  1. 1.

    Oudemansiella raphanipes polysaccharides (OrPs) can significantly regulate the body weight of mice.

  2. 2.

    OrPs plays a prebiotic role in changing the composition and structure of gut microbiota in healthy mice.

  3. 3.

    OrPs significantly increased the content of short-chain fatty acids in feces of mice.

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Availability of data and material

All data were uploaded to the National Center for Biotechnology Information database (Accession Number: PRJNA769810).

Change history

Abbreviations

ASV:

Amplicon sequence variant

BGA:

Brain-gut axis

BW:

Body weight

COGs:

Clusters of Orthologous Groups

ConT:

Control

GC-MS:

Gas chromatography-mass spectrometry

LDA:

Linear discriminant analysis

OrP:

Oudemansiella raphanipes polysaccharide

PCA:

Principal coordinates analysis

PMP:

1-phenyl-3-methyl-5-pyrazolone

RDA:

Redundancy analysis

SCFA:

Short-chain fatty acid

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Acknowledgements

This work supported by the Scientific Research Foundation for Talent Introduction of Southwest Medical University (0903-00040031), the Applied Basic Research Cooperation Project of Luzhou Government-Southwest Medical University (2020LZXNYDJ28). We thank Mr. Yu Luo purchased the mushroom.

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Author notes

  1. Qi Fang and Yong Lai contributed equally to this work.

Authors and Affiliations

  1. School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China

    Qi Fang, Yong Lai, Dan Zhang, Hui Lei, Fang Wang, Xiurong Guo & Can Song

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  1. Qi Fang
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Contributions

C.S. conceived and designed the experiments. Q.F. and Y.L. performed the experiments. C.S., D.Z., H.L., F.W. and X.G. contributed reagents/materials/analysis tools. C.S. analyzed the data and created figures. Q.F. and C.S. wrote the paper. All authors reviewed the manuscript.

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Correspondence to Can Song.

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All experiments were approved by the Ethics Committee of SWMU under permit number 2021060902. Study design and animal specimen collection were carried out and reported following the ARRIVE guidelines (https://arriveguidelines.org/arrive-guidelines).

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Fang, Q., Lai, Y., Zhang, D. et al. Gut microbiota regulation and prebiotic properties of polysaccharides from Oudemansiella raphanipes mushroom. World J Microbiol Biotechnol 39, 167 (2023). https://doi.org/10.1007/s11274-023-03616-1

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