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Purification and biochemical characterization of a novel chitosanase cloned from the gene of Kitasatospora setae KM-6054 and its application in the production of chitooligosaccharides

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Abstract

Chitosanase could degrade chitosan efficiently under mild conditions to prepare chitosan oligosaccharides (COSs). COS possesses versatile physiological activities and has wide application prospects in food, pharmaceutical and cosmetic fields. Herein, a new glycoside hydrolase (GH) family 46 chitosanase (CscB) was cloned from Kitasatospora setae KM-6054 and heterologously expressed in Escherichia coli. The recombinant chitosanase CscB was purified by Ni-charged magnetic beads and showed a relative molecular weight of 29.19 kDa by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE). CscB showed the maximal activity (1094.21 U/mg) at pH 6.0 and 30 °C. It was revealed that CscB is a cold-adapted enzyme. CscB was determined to be an endo-type chitosanase with a polymerization degree of the final product mainly in the range of 2–4. This new cold-adapted chitosanase provides an efficient enzyme tool for clean production of COSs.

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Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The work was supported by the National Natural Science Foundation of China (Grant No. 31601410), Postgraduate Research & Practice Innovation Program of Jiangsu Province (KYCX20_1103). Dr. Zhu Benwei gratefully acknowledges the support of Jiangsu Overseas Visiting Scholar Program for University Prominent Young and Mid-aged Teachers and Presidents.

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  1. College of Food Science and Light Industry, Nanjing Tech University, Nanjing, 211816, People’s Republic of China

    Yinxiao Xu, Hui Wang, Benwei Zhu, Zhong Yao & Ling Jiang

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  1. Yinxiao Xu
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  2. Hui Wang
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  3. Benwei Zhu
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  4. Zhong Yao
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  5. Ling Jiang
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XYX and HW performed the experiments, analyzed the data and wrote the initial draft. BWZ supervised the project, acquire the funding and edited the final draft. ZY and LJ acquired the funding and analyzed some data. All authors read and approved the final manuscript.

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Correspondence to Benwei Zhu.

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Xu, Y., Wang, H., Zhu, B. et al. Purification and biochemical characterization of a novel chitosanase cloned from the gene of Kitasatospora setae KM-6054 and its application in the production of chitooligosaccharides. World J Microbiol Biotechnol 39, 111 (2023). https://doi.org/10.1007/s11274-023-03561-z

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