Abstract
The current study aimed to investigate exopolysaccharides (EPSs) produced by two Antarctic yeasts isolated from soil and penguin feathers samples collected on Livingston Island (Antarctica). The strains were identified as belonging to the species Leucosporidium yakuticum (LY) and Cystobasidium ongulense (CO) based on molecular genetic analysis. The EPS production was investigated using submerged cultivation. Different chemical, chromatographic, and spectral analyses were employed to characterize EPSs. LY accumulated 5.5 g/L biomass and 4.0 g/L EPS after 120 h of cultivation, while CO synthesized 2.1 g/L EPS at the end of cultivation, and the biomass amount reached 5.5 g/L. LY-EPS was characterized by a higher total carbohydrate content (80%) and a lower protein content (18%) by comparison with CO-EPS (62%, 30%). The LY-EPS mainly consisted of mannose (90 mol%), whereas CO-EPS had also glucose, galactose, and small amounts of uronic acids (8–5 mol%). Spectral analyses (FT-IR and 1D, 2D NMR) revealed that LY-EPS comprised a typical β-(1 → 4)-mannan. Branched (hetero)mannan, together with β/α-glucans constituted the majority of CO-EPS. Unlike LY-EPS, which had a high percentage of high molecular weight populations, CO-EPS displayed a large quantity of lower molecular weight fractions and a higher degree of heterogeneity. LY-EPS (100 ng/mL) elevated significantly interferon gamma (IFN-γ) production in splenic murine macrophages and natural killer (NK) cells. The results indicated that newly identified EPSs might affect IFN-γ signaling and in turn, might enhance anti-infectious responses. The data obtained also revealed the potential of EPSs and yeasts for practical application in biochemical engineering and biotechnology.
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References
Aimanianda V, Clavaud C, Simenel C, Fontaine T, Delepierre M, Latge J-P (2009) Cell wall β-(1,6)-glucan of Saccharomyces cerevisiae. Structural characterization and in situ synthesis. J Biol Chem 284:13401–13412. https://doi.org/10.1074/jbc.M807667200
Ali P, Shah A, Hasan F, Hertkorn N, Gonsior M, Sajjad W, Chen F (2020) A glacier bacterium produces high yield of cryoprotective exopolysaccharide. Front Microbiol 10:3096. https://doi.org/10.3389/fmicb.2019.03096
Belitz H-D, Grosch W, Schieberle P (2009) Polysaccharides. In: Belitz H-D, Grosch W, Schieberle P (eds) Food chemistry, 4th edn. Springer, Berlin, pp 296–302
Bicca S, Poncet-Legrand C, Williams P, Nguela J, Doco T, Vernhet A (2022) Structural characteristics of Saccharomyces cerevisiae mannoproteins: impact of their polysaccharide part. Carbohydr Polym 277:118758. https://doi.org/10.1016/j.carbpol.2021.118758
Breierová E, Hromádková Z, Stratilová E, Sasinková V, Ebringerová A (2005) Effect of salt stress on the production and properties of extracellular polysaccharides produced by Cryptococcus laurentii. Z Naturforsch C 60:444–450. https://doi.org/10.1515/znc-2005-5-613
Buzzini P, Margesin R (2014) Cold-Adapted Yeasts: A lesson from the cold and a challenge for the XXI century. In: Buzzini P, Margesin R (eds) Cold-adapted yeasts: biodiversity, adaptation strategies and biotechnological significance. Springer, Berlin, pp 3–22
Chatterjee S, Mukhopadhyay SK, Gauri SS, Dey S (2018) Sphingobactan, a new α-mannan exopolysaccharide from Arctic Sphingobacterium sp. IITKGP-BTPF3 capable of biological response modification. Int Immunopharmacol 60:84–95. https://doi.org/10.1016/j.intimp.2018.04.039
Chen G, Long Yu, Zhang Y, Chang Y, Liu Y, Shen J, Xue C (2021) Utilizing heterologously overexpressed endo-1,3-fucanase to investigate the structure of sulfated fucan from sea cucumber (Holothuria hilla). Carbohydr Polym 272:118480. https://doi.org/10.1016/j.carbpol.2021.118480
Corsaro MM, Lanzetta R, Parrilli E, Parrilli M, Tutino ML, Ummarino S (2004) Influence of growth temperature on lipid and phosphate contents of surface polysaccharides from the Antarctic bacterium Pseudoalteromonas haloplanktis TAC 125. J Bacteriol 186:29–34. https://doi.org/10.1128/JB.186.1.29-34.2004
Donot F, Fontana A, Baccou J, Schorr-Galindo S (2012) Microbial exopolysaccharides: main examples of synthesis, excretion, genetics and extraction. Carbohydr Polym 87:951–962. https://doi.org/10.1016/j.carbpol.2011.08.083
DuBois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356. https://doi.org/10.1021/ac60111a017
Ellefsen CF, Wold CW, Wilkins AL, Rise F, Samuelsen ABC (2021) Water-soluble polysaccharides from Pleurotus eryngii fruiting bodies, their activity and affinity for toll-like receptor 2 and dectin-1. Carbohydr Polym 264:117991. https://doi.org/10.1016/j.carbpol.2021.117991
Freitas F, Alves V, Reis M (2011) Advances in bacterial exopolysaccharides: from production to biotechnological applications. Trends Biotechnol 29:388–398. https://doi.org/10.1016/j.tibtech.2011.03.008
Galinari E, Sabry DA, Sassaki GL, Macedo GR, Passos FML, Mantovani HC, Rocha HAO (2017) Chemical structure, antiproliferative and antioxidant activities of a cell wall α-d-mannan from yeast Kluyveromyces marxianus. Carbohydr Polym 157:1298–1305. https://doi.org/10.1016/j.carbpol.2016.11.015
Ghada SI, Manal GH, Mohsen MS, Eman AG (2012) Production and biological evaluation of exopolysaccharide from isolated Rhodotorula glutinins. Aust J Basic Appl Sci 6:401–408
Gómez-Miranda B, Prieto A, Leal JA, Ahrazem O, Jiménez-Barbero J, Bernabé M (2004) Differences among the cell wall galactomannans from Aspergillus wentii and Chaetosartorya chrysella and that of aspergillus fumigates. Glycoconj J 20:239–246. https://doi.org/10.1023/B:GLYC.0000025818.83019.e4
Gorin PAJ (1973) Rationalization of Carbon-13 magnetic resonance spectra of yeast mannans and structurally related oligosaccharides. Can J Chem 51:2375–2383. https://doi.org/10.1139/v73-356
Gorin PAJ, Spencer JFT, Bhattachar SS (1969) Structures of yeast mannans containing both α- and β-linked D-mannopyranose units. Can J Chem 47:1499–1505. https://doi.org/10.1139/v69-248
Hamidi M, Gholipour AR, Delattre C, Sesdighi F, Seveiri RM, Pasdaran A, Kheirandish S, Pierre G, Kozani PS, Kozani PS, Karimitabar F (2020) Production, characterization and biological activities of exopolysaccharides from a new cold-adapted yeast: Rhodotorula mucilaginosa sp. GUMS16. Int J Biol Macromol 151:268–277. https://doi.org/10.1016/j.ijbiomac.2020.02.206
Han JM, Xu Z, Liu ZM, Qian H, Zhang WG (2018) Co-production of microbial oil and exopolysaccharide by the oleaginous yeast Sporidiobolus pararoseus grown in fed-batch culture. RSC Adv 8:3348–3356. https://doi.org/10.1039/C7RA12813D
Hannuksela T, du Penhoat CH (2004) NMR structural determination of dissolved O-acetylated galactoglucomannan isolated from spruce thermomechanical pulp. Carbohydr Res 339:301–312. https://doi.org/10.1016/j.carres.2003.10.025
Hao Y, Huang Y, Chen J, Li J, Yuan Y, Wang M, Han L, Xin X, Wang H, Lin D, Peng F, Yu F, Zheng C, Shen C (2020) Exopolysaccharide from Cryptococcus heimaeyensis S20 induces autophagic cell death in non-small cell lung cancer cells via ROS/p38 and ROS/ERK signaling. Cell Prolif 53:e12869. https://doi.org/10.1111/cpr.12869
Hristova D, Rusinova-Videva S, Konstantinov S (2021) Biologically active substances and extracts of fungal original. Pharmacong Rev 15:12–19. https://doi.org/10.5530/phrev.2021.15.2
Huo J, Wu J, Sun B, Zhao M, Sun W, Sun J, Huang M (2020) Isolation, purification, structure characterization of a novel glucan from Huangshui, a byproduct of chinese Baijiu, and its immunomodulatory activity in LPS-stimulated THP-1 cells. Int J Biol Macromol 161:406–416. https://doi.org/10.1016/j.ijbiomac.2020.06.028
Kanevskiy LM, Telford WG, Sapozhnikov AM, Kovalenko EI (2013) Lipopolysaccharide induces IFN-γ production in human NK cells. Front Immunol 4:11. https://doi.org/10.3389/fimmu.2013.00011
Karkhanis YD, Zeltner JY, Jackson JJ, Carlo DJ (1978) A new and improved microassay to determine 2-keto-3-deoxyoctonate in lipopolysaccharide of gram-negative bacteria. Anal Biochem 85:595–601. https://doi.org/10.1016/0003-2697(78)90260-9
Kato K, Nitta M, Mizuno T (1973) Infrared spectroscopy of some mannans. Agric Biol Chem 37:433–435. https://doi.org/10.1080/00021369.1973.10860687
Kim HJ, Lee JH, Do H, Jung W (2014) Production of antifreeze proteins by cold-adapted yeasts. In: Buzzini P, Margesin R (eds) Cold-adapted yeasts. Springer, Berlin, pp 259–280
Kobayashi H, Kawakami S, Ogawa Y, Shibata N, Suzuki S (2013) Structural investigation of cell wall mannan antigen obtained from pathogenic yeast Candida zeylanoides. Open J Med Microbiol 3:139–143. https://doi.org/10.4236/ojmm.2013.32021
Komura DL, Carbonero ER, Gracher AHP, Baggio CH, Freitas CS, Marcon R, Santos ARS, Gorin PAJ, Iacomini M (2010) Structure of Agaricus spp. fucogalactans and their anti-inflammatory and antinociceptive properties. Bioresour Technol 101:6192–6199. https://doi.org/10.1016/j.biortech.2010.01.142
Lackovic V, Borecky L, Sikl D, Masler L, Bauer S (1970) Stimulation of interferon production by mannans. Proc Soc Exp Biol Med 134:874–879. https://doi.org/10.3181/00379727-134-34902
Leung M, Liu C, Koon J, Fung K (2006) Polysaccharide biological response modifiers. Immun Lett 105:101–114. https://doi.org/10.1016/j.imlet.2006.01.009
Li Q-Z, Wu D, Zhou S, Liu Y-F, Li Z-P, Feng J, Yang Y (2016) Structure elucidation of a bioactive polysaccharide from fruiting bodies of Hericium erinaceus in different maturation stages. Carbohydr Polym 144:196–204. https://doi.org/10.1016/j.carbpol.2016.02.051
Li A, Yuan F, Groenewald M, Bensch K, Yurkov A, Li K, Han P, Guo L, Aime M, Sampaio J, Jindamorakot S, Turchetti B, Inacio J, Fungsin B, Wang Q, Bai F (2020) Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: proposal of two new orders, three new families, eight new genera and one hundred and seven new species. Stud Micol 96:17–140. https://doi.org/10.1016/j.simyco.2020.01.002
Liu X, Renard CMGC, Bureau S, Le Bourvellec C (2021) Revisiting the contribution of ATR-FTIR spectroscopy to characterize plant cell wall polysaccharides. Carbohydr Polym 262:117935. https://doi.org/10.1016/j.carbpol.2021.117935
Ma W, Chen X, Wang B, Lou W, Chen X, Hua J, Sun Yj, Zhao Y, Peng T (2018) Characterization, antioxidativity, and anticarcinoma activity of exopolysaccharide extract from Rhodotorula mucilaginosa CICC 33013. Carbohydr Polym 181:768–777. https://doi.org/10.1016/j.carbpol.2017.11.080
Makarova EN, Patova OA, Shakhmatov EG, Kuznetsov SP, Ovodov YS (2013) Structural studies of the pectic polysaccharide from siberian fir (Abies sibirica Ledeb.). Carbohydr Polym 92:1817–1826. https://doi.org/10.1016/j.carbpol.2012.11.038
Makarova EN, Shakhmatov EG, Belyy VA (2018) Structural studies of water-extractable pectic polysaccharides and arabinogalactan proteins from Picea abies greenery. Carbohydr Polym 195:207–217. https://doi.org/10.1016/j.carbpol.2018.04.074
Martorell M, Ruberto L, Figueroa L, Cormack W (2019) Antarctic yeasts as a source of enzymes for biotechnological applications. In: Rosa L (ed) Fungi of Antarctica. Springer, Switzerland, pp 285–304
Matsuo K, Isogai E, Araki Y (2000) Occurrence of [→3)-β-D-Manp-(1→4)-β-D-Manp-(1→]n units in the antigenic polysaccharides from Leptospira biflexa serovar patoc strain patoc I. Carbohydr Res 328:517–524. https://doi.org/10.1016/S0008-6215(00)00143-9
McComb EA, McCready RM (1957) Determination of acetyl in pectin and in acetylated carbohydrate polymers. Anal Chem 29:819–821. https://doi.org/10.1021/ac60125a025
Murphy EA, Davis JM, Brown AS, Carmichael MD, Ghaffar A, Mayer EP (2007) Oat beta-glucan effects on neutrophil respiratory burst activity following exercise. Med Sci Sport Exer 39:639–644. https://doi.org/10.1249/mss.0b013e3180306309
Nandi AK, Samanta S, Sen IK, Devi KSP, Maiti TK, Acharya K, Islam SS (2013) Structural elucidation of an immunoenhancing heteroglycan isolated from Russula albonigra. (Krombh) Fr Carbohydr Polym 94:918–926. https://doi.org/10.1016/j.carbpol.2013.02.019
Naumenko OI, Zheng H, Wang J, Senchenkova SN, Wang H, Shashkov AS, Chizhov AO, Li Q, Knirel YA, Xiong Y (2018) Structure elucidation of the O-specific polysaccharide by NMR spectroscopy and selective cleavage and genetic characterization of the O-antigen of Escherichia albertii O5. Carbohydr Res 457:25–31. https://doi.org/10.1016/j.carres.2017.12.010
NFSS (2010) Determination of Protein in Foods. National Food Safety Standard of the People’s Republic of China. China National Center for Food Safety Risk Assessment, Beijing. GB 5009.5–2010
Nicolaus B, Kambourova M, Oner E (2010) Exopolysaccharides from extremophiles: from fundamentals to biotechnology. Environ Technol 31:1145–1158. https://doi.org/10.1080/09593330903552094
Ognyanov M, Georgiev Y, Petkova N, Ivanov I, Vasileva I, Kratchanova M (2018) Isolation and characterization of pectic polysaccharide fraction from in vitro suspension culture of Fumaria officinalis L. Int J Polym Sci 2018:5705036. https://doi.org/10.1155/2018/5705036
Panikov NS (2014) Subzero activity of cold-adapted yeasts. In: Buzzini P, Margesin R (eds) Cold-adapted yeasts. Springer, Berlin, pp 295–323
Pavlova K (2014) Production of polymers and other compounds of industrial importance by cold-adapted yeasts. In: Buzzini P, Margesin R (eds) Cold-adapted yeasts. Springer, Berlin, pp 397–415
Pavlova K, Koleva L, Kratchanova M, Panchev I (2004) Production and characterization of an exopolysaccharide by yeast. World J Microbiol Biotechnol 20:435–439. https://doi.org/10.1023/B:WIBI.0000033068.45655.2a
Pavlova K, Panchev I, Kratchanova M, Gocheva M (2009) Production of an exopolysaccharide by Antarctic yeast. Folia Microbiol (Praha) 54:343–348. https://doi.org/10.1007/s12223-009-0049-y
Pavlova K, Rusinova-Videva S, Kuncheva M, Kratchanova M, Gocheva M, Dimitrova S (2011) Synthesis and characterization of an exopolysaccharide from Antarctic yeast strain Cryptococcus laurentii AL100. Appl Biochem Biotechnol 163:1038–1052. https://doi.org/10.1007/s12010-010-9107-9
Poli A, Anzelmo G, Tommonaro G, Pavlova K, Casaburi A, Nicolaus B (2010) Production and chemical characterization of an exopolysaccharide synthesized by psychrophilic yeast strain Sporobolomyces salmonicolor AL1 isolated from Livingston Island, Antarctica. Folia Microbiol 55:576–581. https://doi.org/10.1007/s12223-010-0092-8
Previato JO, Vinogradov E, Silva MAE, Oliveira PAV, Fonseca LM, Maes E, Mendonça-Previato L (2019) Characterization of the 6-O-acetylated lipoglucuronomannogalactan a novel Cryptococcus neoformans cell wall polysaccharide. Carbohydr Res 475:1–10. https://doi.org/10.1016/j.carres.2019.01.012
Ragavan ML, Das N (2019) Optimization of exopolysaccharide production by probiotic yeast Lipomyces starkeyi VIT-MN03 using response surface methodology and its applications. Annal Microbiol 69:515–530. https://doi.org/10.1007/s13213-019-1440-9
Rusinova-Videva S, Pavlova K, Georgieva K (2011) Effect of different carbon sources on biosynthesis of exopolysaccharide from Antarctic strain Cryptococcus laurentii AL62. Biotechn Biotechnol Eq 25: 80–84. https://doi.org/10.5504/BBEQ.2011.0121
Reyes-Becerril M, Angulo M, Sanchez V, Machuca C, Mendez-Martinez Y, Angulo C (2021) β–glucan bioactivities from Cystobasidium benthicum in Totoaba macdonaldi thymus cells. Fish Shellfish Immunol 119: 542–553. https://doi.org/10.1016/j.fsi.2021.10.042
Rusinova-Videva S, Kambourova M, Alipieva K, Nachkova S, Simova S (2019) Metabolic profiling of Antarctic yeasts by proton nuclear magnetic resonance-based spectroscopy. Biotechnol Biotechnol Eq. 33:12–19. https://doi.org/10.1080/13102818.2018.1490201
Rusinova-Videva S, Nachkova S, Adamov A, Dimitrova-Dyulgerova I (2020) Antarctic yeast Cryptococcus laurentii (AL65): biomass and exopolysaccharide production and biosorption of metals. J Chem Technol Biotech 95:1372–1379. https://doi.org/10.1002/jctb.6321
Rusinova-Videva S, Ognyanov M, Georgiev Y, Kambourova M, Adamov A, Krasteva V (2022) Production and chemical characterization of exopolysaccharides by Antarctic yeast Vishniacozyma victoriae and Tremellomycetes sp. Appl Sci 12:1805. https://doi.org/10.3390/app12041805
Saeed A, Paściak M, Górska S, Ceremuga I, Gamian E, Ziółkowski P, Drab M, Gamian A (2018) Structural elucidation of Tsukamurella pulmonis neutral polysaccharide and its visualization in infected mouse tissues by specific monoclonal antibodies. Sci Rep 8:11564. https://doi.org/10.1038/s41598-018-29864-y
Samuelsen ABC, Rise F, Wilkins AL, Teveleva L, Nyman AAT, Aachmann FL (2019) The edible mushroom Albatrellus ovinus contains a α-L-fuco-α-D-galactan, α-D-glucan, a branched (1→6)-β-D-glucan and a branched (1→3)-β-D-glucan. Carbohydr Res 471:28–38. https://doi.org/10.1016/j.carres.2018.10.012
Sanchez V, Rosales-Mendosa S, Monreal-Escalante E, Murillo-Alvarez J, Angulo C (2021) Conjugation of β-glucans on heat-stable enterotoxins (ST) to enhance the immunogenic response in mouse leucocytes. Mater Sci Eng C 118:111464. https://doi.org/10.1016/j.msec.2020.111464
Seveiri MR, Hamidi M, Delattre C, Rahmani B, Darzi S, Pierre G, Sedighian H (2019) Characterization of the exopolysaccharides from Rhodotorula minuta IBRC-M 30135 and evaluation of their emulsifying, antioxidant and antiproliferative activities. Med Sci 23:381–389
Shakhmatov EG, Makarova EN (2022) Structure of KOH-extractable polysaccharides of tree greenery from siberian fir Abies sibirica Ledeb. Carbohydr Polym 276:118794. https://doi.org/10.1016/j.carbpol.2021.118794
Shibata N, Ikuta K, Imai T, Satoh Y, Satoh R, Suzuki A, Kojima C, Kobayashi H, Hisamichi K, Suzuki S (1995) Existence of branched side chains in the cell wall mannan of pathogenic yeast, Candida albicans. Structure-antigenicity relationship between the cell wall mannans of Candida albicans and Candida parapsilosis. J Biol Chem 270:1113–1122. https://doi.org/10.1074/jbc.270.3.1113
Synytsya A, Cŏpíková J, Matějka P, Machovič V (2003) Fourier transform Raman and infrared spectroscopy of pectins. Carbohydr Polym 54:97–106. https://doi.org/10.1016/S0144-8617(03)00158-9
Tang N, Wang X, Yang R, Liu Z, Liu Y, Tian J, Xiao L, Li W (2022) Extraction, isolation, structural characterization and prebiotic activity of cell wall polysaccharide from Kluyveromyces marxianus. Carbohydr Polym 289:119457. https://doi.org/10.1016/j.carbpol.2022.119457
Tsuji М, Tsujimoto M, Imura S (2017) Cystobasidium tubakii and cystobasidium ongulense, new basidiomycetous yeast species isolated from East Ongul Island, East Antarctica. Micoscience 58:103–110. https://doi.org/10.1016/j.myc.2016.11.002
Turkiewicz M, Pazgier M, Kalinowska H, Bielecki S (2003) A cold-adapted extracellular serine proteinase of the yeast Leucosporidium antarcticum. Extremophiles 7:435–442. https://doi.org/10.1007/s00792-003-0340-9
Turkiewicz M, Pazgier M, Donachie SP, Kalinowska H (2005) Invertase and α-glucosidase production by the endemic antarctic marine yeast Leucosporidium antarcticum. Pol Polar Res 26:125–136
Ustyuzhanina NE, Kulakovskaya EV, Kulakovskaya TV, Menshov VM, Dmitrenok AS, Shashkov AS, Nifantiev NE (2018) Mannan and phosphomannan from Kuraishia capsulata yeast. Carbohydr Polym 181:624–632. https://doi.org/10.1016/j.carbpol.2017.11.103
Valente P, Gouveia F, de Lemos G, Pimentel D, van Elsas J, Mendonca-Hagler L, Hagler A (1996) PCR amplification of the rDNA internal transcribed spacer region for differentiation of Saccharomyces cultures. FEMS Microbiol Lett 137:253–256. https://doi.org/10.1111/j.1574-6968.1996.tb08114.x
Van Bogaert INA, De Maeseneire SL, Vandamme EJ (2009) Extracellular polysaccharides produced by yeasts and yeast-like fungi. In: Satyanarayana T, Kunze G (eds) Yeast biotechnology: diversity and applications. Springer, Berlin, pp 651–671
Vishniac HS (2006) Yeast biodiversity in the Antarctic. In: Rosa CA, Peter G (eds) Biodiversity and ecophysiology of yeasts. Springer, Berlin, pp 420–440
Wiley SR, Schooley K, Smolak PJ, Din WS, Huang CP, Nicholl JK, Sutherland GR, Smith TD, Rauch C, Smith CA, Goodwin RG (1995) Identification and characterization of a new member of the TNF family that induces apoptosis. Immunity 3:673–682. https://doi.org/10.1016/1074-7613(95)90057-8
Yamada H, Ohshima Y, Miyazaki T (1982) Characterisation of fucomannopeptide and mannoprotein from Absidia cylindrospora. Carbohydr Res 110:113–126. https://doi.org/10.1016/0008-6215(82)8
Yang L, Zhang L-M (2009) Chemical structural and chain conformational characterization of some bioactive polysaccharides isolated from natural sources. Carbohydr Polym 76:349–361. https://doi.org/10.1016/j.carbpol.2008.12.015
Zhang Z, Zhou Z, Li Y, Zhou L, Ding Q, Xu L (2016) Isolated exopolysaccharides from Lactobacillus rhamnosus GG alleviated adipogenesis mediated by TLR2 in mice. Sci Rep 6:36083. https://doi.org/10.1038/srep36083
Zhang S, Qiao Z, Zhao Z, Guo J, Lu K, Mayo KH, Zhou Y (2021) Comparative study on the structures of intra- and extra-cellular polysaccharides from Penicillium oxalicum and their inhibitory effects on galectins. Int J Biol Macromol 181:793–800. https://doi.org/10.1016/j.ijbiomac.2021.04.042
Zhang M, Qin H, An R, Zhang W, Liu J, Yu Q, Liu W, Huang X (2022) Isolation, purification, structural characterization and antitumor activities of a polysaccharide from Lilium davidii var. Unicolor cotton. J Mol Struct 1261:132941. https://doi.org/10.1016/j.molstruc.2022.132941
Zhou X, Hong T, Yu Q, Nie S, Gong D, Xiong T, Xie M (2017) Exopolysaccharides from Lactobacillus plantarum NCU116 induce c-Jun dependent Fas/Fasl-mediated apoptosis via TLR2 in mouse intestinal epithelial cancer cells. Sci Rep 7:14247. https://doi.org/10.1038/s41598-017-14178-2
Zhou Y, Cui Y, Qu X (2019) Exopolysaccharides of lactic acid bacteria: structure, bioactivity and associations: a review. Carbohydr Polym 207:317–332. https://doi.org/10.1016/j.carbpol.2018.11.093
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We would like to thank the staff of the Bulgarian Antarctic Expedition for their logistic support. The study was supported by Project 70.25–173/ 22.11.2019 of Sofia University “St. Kliment Ohridski” and the National Centre for Polar Studies in the frames of the National Program for Polar Studies 2017–2021, funded by the Bulgarian Ministry of Education and Science.
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Rusinova-Videva, S., Ognyanov, M., Georgiev, Y. et al. Chemical characterization and biological effect of exopolysaccharides synthesized by Antarctic yeasts Cystobasidium ongulense AL101 and Leucosporidium yakuticum AL102 on murine innate immune cells. World J Microbiol Biotechnol 39, 39 (2023). https://doi.org/10.1007/s11274-022-03477-0
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DOI: https://doi.org/10.1007/s11274-022-03477-0